Standard
High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach. / Mollerup, Sarah; Asplund, Maria; Friis-Nielsen, Jens; Kjartansdóttir, Kristín Rós; Fridholm, Helena; Hansen, Thomas Arn; Herrera, José Alejandro Romero; Barnes, Christopher James; Jensen, Randi Holm; Richter, Stine Raith; Nielsen, Ida Broman; Pietroni, Carlotta; Alquezar-Planas, David E; Rey-Iglesia, Alba; Olsen, Pernille V S; Rajpert-De Meyts, Ewa; Groth-Pedersen, Line; von Buchwald, Christian; Jensen, David H; Gniadecki, Robert; Høgdall, Estrid; Langhoff, Jill Levin; Pete, Imre; Vereczkey, Ildikó; Baranyai, Zsolt; Dybkaer, Karen; Johnsen, Hans Erik; Steiniche, Torben; Hokland, Peter; Rosenberg, Jacob; Baandrup, Ulrik; Sicheritz-Pontén, Thomas; Willerslev, Eske; Brunak, Søren; Lund, Ole; Mourier, Tobias; Vinner, Lasse; Izarzugaza, Jose M G; Nielsen, Lars Peter; Hansen, Anders Johannes.
In:
The Journal of Infectious Diseases, Vol. 220, No. 8, 15.10.2019, p. 1312-1324.
Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
Mollerup, S, Asplund, M, Friis-Nielsen, J, Kjartansdóttir, KR, Fridholm, H, Hansen, TA, Herrera, JAR
, Barnes, CJ, Jensen, RH, Richter, SR
, Nielsen, IB, Pietroni, C, Alquezar-Planas, DE
, Rey-Iglesia, A, Olsen, PVS, Rajpert-De Meyts, E, Groth-Pedersen, L
, von Buchwald, C, Jensen, DH, Gniadecki, R
, Høgdall, E, Langhoff, JL, Pete, I, Vereczkey, I, Baranyai, Z, Dybkaer, K, Johnsen, HE, Steiniche, T, Hokland, P
, Rosenberg, J, Baandrup, U
, Sicheritz-Pontén, T, Willerslev, E, Brunak, S, Lund, O, Mourier, T
, Vinner, L, Izarzugaza, JMG, Nielsen, LP
& Hansen, AJ 2019, '
High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach',
The Journal of Infectious Diseases, vol. 220, no. 8, pp. 1312-1324.
https://doi.org/10.1093/infdis/jiz318APA
Mollerup, S., Asplund, M., Friis-Nielsen, J., Kjartansdóttir, K. R., Fridholm, H., Hansen, T. A., Herrera, J. A. R.
, Barnes, C. J., Jensen, R. H., Richter, S. R.
, Nielsen, I. B., Pietroni, C., Alquezar-Planas, D. E.
, Rey-Iglesia, A., Olsen, P. V. S., Rajpert-De Meyts, E., Groth-Pedersen, L.
, von Buchwald, C., Jensen, D. H.
, ... Hansen, A. J. (2019).
High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach.
The Journal of Infectious Diseases,
220(8), 1312-1324.
https://doi.org/10.1093/infdis/jiz318Vancouver
Mollerup S, Asplund M, Friis-Nielsen J, Kjartansdóttir KR, Fridholm H, Hansen TA et al.
High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach.
The Journal of Infectious Diseases. 2019 Oct 15;220(8):1312-1324.
https://doi.org/10.1093/infdis/jiz318Author
Mollerup, Sarah ; Asplund, Maria ; Friis-Nielsen, Jens ; Kjartansdóttir, Kristín Rós ; Fridholm, Helena ; Hansen, Thomas Arn ; Herrera, José Alejandro Romero ; Barnes, Christopher James ; Jensen, Randi Holm ; Richter, Stine Raith ; Nielsen, Ida Broman ; Pietroni, Carlotta ; Alquezar-Planas, David E ; Rey-Iglesia, Alba ; Olsen, Pernille V S ; Rajpert-De Meyts, Ewa ; Groth-Pedersen, Line ; von Buchwald, Christian ; Jensen, David H ; Gniadecki, Robert ; Høgdall, Estrid ; Langhoff, Jill Levin ; Pete, Imre ; Vereczkey, Ildikó ; Baranyai, Zsolt ; Dybkaer, Karen ; Johnsen, Hans Erik ; Steiniche, Torben ; Hokland, Peter ; Rosenberg, Jacob ; Baandrup, Ulrik ; Sicheritz-Pontén, Thomas ; Willerslev, Eske ; Brunak, Søren ; Lund, Ole ; Mourier, Tobias ; Vinner, Lasse ; Izarzugaza, Jose M G ; Nielsen, Lars Peter ; Hansen, Anders Johannes. / High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach. In: The Journal of Infectious Diseases. 2019 ; Vol. 220, No. 8. pp. 1312-1324.
Bibtex
@article{c48f709ff48a4edd80bee3e4e62ff20d,
title = "High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach",
abstract = "BACKGROUND: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.",
author = "Sarah Mollerup and Maria Asplund and Jens Friis-Nielsen and Kjartansd{\'o}ttir, {Krist{\'i}n R{\'o}s} and Helena Fridholm and Hansen, {Thomas Arn} and Herrera, {Jos{\'e} Alejandro Romero} and Barnes, {Christopher James} and Jensen, {Randi Holm} and Richter, {Stine Raith} and Nielsen, {Ida Broman} and Carlotta Pietroni and Alquezar-Planas, {David E} and Alba Rey-Iglesia and Olsen, {Pernille V S} and {Rajpert-De Meyts}, Ewa and Line Groth-Pedersen and {von Buchwald}, Christian and Jensen, {David H} and Robert Gniadecki and Estrid H{\o}gdall and Langhoff, {Jill Levin} and Imre Pete and Ildik{\'o} Vereczkey and Zsolt Baranyai and Karen Dybkaer and Johnsen, {Hans Erik} and Torben Steiniche and Peter Hokland and Jacob Rosenberg and Ulrik Baandrup and Thomas Sicheritz-Pont{\'e}n and Eske Willerslev and S{\o}ren Brunak and Ole Lund and Tobias Mourier and Lasse Vinner and Izarzugaza, {Jose M G} and Nielsen, {Lars Peter} and Hansen, {Anders Johannes}",
note = "{\textcopyright} The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.",
year = "2019",
month = oct,
day = "15",
doi = "10.1093/infdis/jiz318",
language = "English",
volume = "220",
pages = "1312--1324",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "8",
}
RIS
TY - JOUR
T1 - High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach
AU - Mollerup, Sarah
AU - Asplund, Maria
AU - Friis-Nielsen, Jens
AU - Kjartansdóttir, Kristín Rós
AU - Fridholm, Helena
AU - Hansen, Thomas Arn
AU - Herrera, José Alejandro Romero
AU - Barnes, Christopher James
AU - Jensen, Randi Holm
AU - Richter, Stine Raith
AU - Nielsen, Ida Broman
AU - Pietroni, Carlotta
AU - Alquezar-Planas, David E
AU - Rey-Iglesia, Alba
AU - Olsen, Pernille V S
AU - Rajpert-De Meyts, Ewa
AU - Groth-Pedersen, Line
AU - von Buchwald, Christian
AU - Jensen, David H
AU - Gniadecki, Robert
AU - Høgdall, Estrid
AU - Langhoff, Jill Levin
AU - Pete, Imre
AU - Vereczkey, Ildikó
AU - Baranyai, Zsolt
AU - Dybkaer, Karen
AU - Johnsen, Hans Erik
AU - Steiniche, Torben
AU - Hokland, Peter
AU - Rosenberg, Jacob
AU - Baandrup, Ulrik
AU - Sicheritz-Pontén, Thomas
AU - Willerslev, Eske
AU - Brunak, Søren
AU - Lund, Ole
AU - Mourier, Tobias
AU - Vinner, Lasse
AU - Izarzugaza, Jose M G
AU - Nielsen, Lars Peter
AU - Hansen, Anders Johannes
N1 - © The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.
PY - 2019/10/15
Y1 - 2019/10/15
N2 - BACKGROUND: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.
AB - BACKGROUND: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.
U2 - 10.1093/infdis/jiz318
DO - 10.1093/infdis/jiz318
M3 - Journal article
C2 - 31253993
VL - 220
SP - 1312
EP - 1324
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 8
ER -
