High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems
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High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems. / Mosgaard, Mette D; Sassene, Philip J; Mu, Huiling; Rades, Thomas; Müllertz, Anette.
In: Journal of Pharmaceutical Sciences, Vol. 106, No. 4, 04.2017, p. 1183-1186.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems
AU - Mosgaard, Mette D
AU - Sassene, Philip J
AU - Mu, Huiling
AU - Rades, Thomas
AU - Müllertz, Anette
N1 - Copyright © 2017 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.
PY - 2017/4
Y1 - 2017/4
N2 - The high-throughput in vitro intestinal lipolysis model (HTP) applicable for rapid and low-scale screening of lipid-based drug delivery systems (LbDDSs) was optimized and adjusted as to be conducted in 96-well plates (HTP-96). Three different LbDDSs (I-III) loaded with danazol or cinnarizine were used as model systems. The distributions of cinnarizine and danazol in the aqueous and precipitated digestion phases generated during lipolysis in HTP-96 were compared with previously published data obtained from HTP. The final HTP-96 setup resulted in the same rank order as the original HTP model with regard to solubilization in the aqueous phase during digestion: LbDDS III > LbDDS II > LbDDS I for danazol and LbDDS III ≈ LbDDS II ≈ LbDDS I for cinnarizine. HTP-96 is a useful model for fast performance assessment of LbDDS in a small scale.
AB - The high-throughput in vitro intestinal lipolysis model (HTP) applicable for rapid and low-scale screening of lipid-based drug delivery systems (LbDDSs) was optimized and adjusted as to be conducted in 96-well plates (HTP-96). Three different LbDDSs (I-III) loaded with danazol or cinnarizine were used as model systems. The distributions of cinnarizine and danazol in the aqueous and precipitated digestion phases generated during lipolysis in HTP-96 were compared with previously published data obtained from HTP. The final HTP-96 setup resulted in the same rank order as the original HTP model with regard to solubilization in the aqueous phase during digestion: LbDDS III > LbDDS II > LbDDS I for danazol and LbDDS III ≈ LbDDS II ≈ LbDDS I for cinnarizine. HTP-96 is a useful model for fast performance assessment of LbDDS in a small scale.
KW - Journal Article
U2 - 10.1016/j.xphs.2016.12.026
DO - 10.1016/j.xphs.2016.12.026
M3 - Journal article
C2 - 28057543
VL - 106
SP - 1183
EP - 1186
JO - Journal of Pharmaceutical Sciences
JF - Journal of Pharmaceutical Sciences
SN - 0022-3549
IS - 4
ER -
ID: 185745485