High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage

Research output: Contribution to journal › Journal article › Research › peer-review

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High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage. / Slaymaker, Ian M; Mesa, Pablo; Kellner, Max J; Kannan, Soumya; Brignole, Edward; Koob, Jeremy; Feliciano, Patricia R; Stella, Stefano; Abudayyeh, Omar O; Gootenberg, Jonathan S; Strecker, Jonathan; Montoya, Guillermo; Zhang, Feng.

In: Cell Reports, Vol. 26, No. 13, 2019, p. 3741-3751.e5.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Slaymaker, IM, Mesa, P, Kellner, MJ, Kannan, S, Brignole, E, Koob, J, Feliciano, PR, Stella, S, Abudayyeh, OO, Gootenberg, JS, Strecker, J, Montoya, G & Zhang, F 2019, 'High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage', Cell Reports, vol. 26, no. 13, pp. 3741-3751.e5. https://doi.org/10.1016/j.celrep.2019.02.094

APA

Slaymaker, I. M., Mesa, P., Kellner, M. J., Kannan, S., Brignole, E., Koob, J., Feliciano, P. R., Stella, S., Abudayyeh, O. O., Gootenberg, J. S., Strecker, J., Montoya, G., & Zhang, F. (2019). High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage. Cell Reports, 26(13), 3741-3751.e5. https://doi.org/10.1016/j.celrep.2019.02.094

Vancouver

Slaymaker IM, Mesa P, Kellner MJ, Kannan S, Brignole E, Koob J et al. High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage. Cell Reports. 2019;26(13):3741-3751.e5. https://doi.org/10.1016/j.celrep.2019.02.094

Author

Slaymaker, Ian M ; Mesa, Pablo ; Kellner, Max J ; Kannan, Soumya ; Brignole, Edward ; Koob, Jeremy ; Feliciano, Patricia R ; Stella, Stefano ; Abudayyeh, Omar O ; Gootenberg, Jonathan S ; Strecker, Jonathan ; Montoya, Guillermo ; Zhang, Feng. / High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage. In: Cell Reports. 2019 ; Vol. 26, No. 13. pp. 3741-3751.e5.

Bibtex

@article{57e81fd06e5c4a1c82cacd7b8455a74e,
title = "High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage",
abstract = "Type VI CRISPR-Cas systems contain programmable single-effector RNA-guided RNases, including Cas13b, one of the four known family members. Cas13b, which has been used for both RNA editing and nucleic acid detection, is unique among type VI CRISPR effectors in its linear domain architecture and CRISPR RNA (crRNA) structure. Here, we report the crystal structure of Prevotella buccae Cas13b (PbuCas13b) bound to crRNA at 1.65 {\AA} resolution. This structure, combined with biochemical experiments assaying the stability, kinetics, and function of Cas13b, provides a mechanistic model for Cas13b target RNA recognition and identifies features responsible for target and cleavage specificity. Based on these observations, we generated Cas13b variants with altered cleavage preferences, which may expand the utility of nuclease-based RNA detection assays and other applications of Cas13b in mammalian cells.",
author = "Slaymaker, {Ian M} and Pablo Mesa and Kellner, {Max J} and Soumya Kannan and Edward Brignole and Jeremy Koob and Feliciano, {Patricia R} and Stefano Stella and Abudayyeh, {Omar O} and Gootenberg, {Jonathan S} and Jonathan Strecker and Guillermo Montoya and Feng Zhang",
year = "2019",
doi = "10.1016/j.celrep.2019.02.094",
language = "English",
volume = "26",
pages = "3741--3751.e5",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "13",

}

RIS

TY - JOUR

T1 - High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage

AU - Slaymaker, Ian M

AU - Mesa, Pablo

AU - Kellner, Max J

AU - Kannan, Soumya

AU - Brignole, Edward

AU - Koob, Jeremy

AU - Feliciano, Patricia R

AU - Stella, Stefano

AU - Abudayyeh, Omar O

AU - Gootenberg, Jonathan S

AU - Strecker, Jonathan

AU - Montoya, Guillermo

AU - Zhang, Feng

PY - 2019

Y1 - 2019

N2 - Type VI CRISPR-Cas systems contain programmable single-effector RNA-guided RNases, including Cas13b, one of the four known family members. Cas13b, which has been used for both RNA editing and nucleic acid detection, is unique among type VI CRISPR effectors in its linear domain architecture and CRISPR RNA (crRNA) structure. Here, we report the crystal structure of Prevotella buccae Cas13b (PbuCas13b) bound to crRNA at 1.65 Å resolution. This structure, combined with biochemical experiments assaying the stability, kinetics, and function of Cas13b, provides a mechanistic model for Cas13b target RNA recognition and identifies features responsible for target and cleavage specificity. Based on these observations, we generated Cas13b variants with altered cleavage preferences, which may expand the utility of nuclease-based RNA detection assays and other applications of Cas13b in mammalian cells.

AB - Type VI CRISPR-Cas systems contain programmable single-effector RNA-guided RNases, including Cas13b, one of the four known family members. Cas13b, which has been used for both RNA editing and nucleic acid detection, is unique among type VI CRISPR effectors in its linear domain architecture and CRISPR RNA (crRNA) structure. Here, we report the crystal structure of Prevotella buccae Cas13b (PbuCas13b) bound to crRNA at 1.65 Å resolution. This structure, combined with biochemical experiments assaying the stability, kinetics, and function of Cas13b, provides a mechanistic model for Cas13b target RNA recognition and identifies features responsible for target and cleavage specificity. Based on these observations, we generated Cas13b variants with altered cleavage preferences, which may expand the utility of nuclease-based RNA detection assays and other applications of Cas13b in mammalian cells.

U2 - 10.1016/j.celrep.2019.02.094

DO - 10.1016/j.celrep.2019.02.094

M3 - Journal article

C2 - 30917325

VL - 26

SP - 3741-3751.e5

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 13

ER -

ID: 216017212