High‐Performance Reversed‐Phase Flash Chromatography Purification of Peptides and Chemically Modified Insulins

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

High‐Performance Reversed‐Phase Flash Chromatography Purification of Peptides and Chemically Modified Insulins. / Sørensen, Kasper K.; Mishra, Narendra K.; Paprocki, Maciej P.; Mehrotra, Amit; Jensen, Knud J.

In: ChemBioChem, Vol. 22, No. 10, 2021, p. 1818–1822.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sørensen, KK, Mishra, NK, Paprocki, MP, Mehrotra, A & Jensen, KJ 2021, 'High‐Performance Reversed‐Phase Flash Chromatography Purification of Peptides and Chemically Modified Insulins', ChemBioChem, vol. 22, no. 10, pp. 1818–1822. https://doi.org/10.1002/cbic.202000826

APA

Sørensen, K. K., Mishra, N. K., Paprocki, M. P., Mehrotra, A., & Jensen, K. J. (2021). High‐Performance Reversed‐Phase Flash Chromatography Purification of Peptides and Chemically Modified Insulins. ChemBioChem, 22(10), 1818–1822. https://doi.org/10.1002/cbic.202000826

Vancouver

Sørensen KK, Mishra NK, Paprocki MP, Mehrotra A, Jensen KJ. High‐Performance Reversed‐Phase Flash Chromatography Purification of Peptides and Chemically Modified Insulins. ChemBioChem. 2021;22(10):1818–1822. https://doi.org/10.1002/cbic.202000826

Author

Sørensen, Kasper K. ; Mishra, Narendra K. ; Paprocki, Maciej P. ; Mehrotra, Amit ; Jensen, Knud J. / High‐Performance Reversed‐Phase Flash Chromatography Purification of Peptides and Chemically Modified Insulins. In: ChemBioChem. 2021 ; Vol. 22, No. 10. pp. 1818–1822.

Bibtex

@article{4d5b704d4bda4d7db25d5bdd4004d673,
title = "High‐Performance Reversed‐Phase Flash Chromatography Purification of Peptides and Chemically Modified Insulins",
abstract = "Preparative reversed-phase HPLC is the established method for the purification of peptides, but has significant limitations. We systematically investigated the use of high-performance reversed-phase flash chromatography (HPFC) to rapidly purify laboratory-scale quantities of crude, synthetic peptides and chemically modified insulins. We demonstrated these methods for a diverse set of peptides, including short, medium, and long peptides. Depending on the purity profile of the peptide, HPFC can be used either as the sole purification method, or as a pre-purification method prior to final HPLC purification. Furthermore, HPFC is suitable for the purification of peptides that are not fully in solution. We provide guidelines for the HPFC of synthetic peptides and small proteins, including the choice of columns, eluents, and gradients. We believe that HPFC is a valuable alternative to HPLC purification of peptides and small proteins.",
author = "S{\o}rensen, {Kasper K.} and Mishra, {Narendra K.} and Paprocki, {Maciej P.} and Amit Mehrotra and Jensen, {Knud J.}",
year = "2021",
doi = "10.1002/cbic.202000826",
language = "English",
volume = "22",
pages = "1818–1822",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "10",

}

RIS

TY - JOUR

T1 - High‐Performance Reversed‐Phase Flash Chromatography Purification of Peptides and Chemically Modified Insulins

AU - Sørensen, Kasper K.

AU - Mishra, Narendra K.

AU - Paprocki, Maciej P.

AU - Mehrotra, Amit

AU - Jensen, Knud J.

PY - 2021

Y1 - 2021

N2 - Preparative reversed-phase HPLC is the established method for the purification of peptides, but has significant limitations. We systematically investigated the use of high-performance reversed-phase flash chromatography (HPFC) to rapidly purify laboratory-scale quantities of crude, synthetic peptides and chemically modified insulins. We demonstrated these methods for a diverse set of peptides, including short, medium, and long peptides. Depending on the purity profile of the peptide, HPFC can be used either as the sole purification method, or as a pre-purification method prior to final HPLC purification. Furthermore, HPFC is suitable for the purification of peptides that are not fully in solution. We provide guidelines for the HPFC of synthetic peptides and small proteins, including the choice of columns, eluents, and gradients. We believe that HPFC is a valuable alternative to HPLC purification of peptides and small proteins.

AB - Preparative reversed-phase HPLC is the established method for the purification of peptides, but has significant limitations. We systematically investigated the use of high-performance reversed-phase flash chromatography (HPFC) to rapidly purify laboratory-scale quantities of crude, synthetic peptides and chemically modified insulins. We demonstrated these methods for a diverse set of peptides, including short, medium, and long peptides. Depending on the purity profile of the peptide, HPFC can be used either as the sole purification method, or as a pre-purification method prior to final HPLC purification. Furthermore, HPFC is suitable for the purification of peptides that are not fully in solution. We provide guidelines for the HPFC of synthetic peptides and small proteins, including the choice of columns, eluents, and gradients. We believe that HPFC is a valuable alternative to HPLC purification of peptides and small proteins.

U2 - 10.1002/cbic.202000826

DO - 10.1002/cbic.202000826

M3 - Journal article

C2 - 33443297

VL - 22

SP - 1818

EP - 1822

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 10

ER -

ID: 261049681