High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato. / Johansen, Ida Elisabeth; Liu, Ying; Jørgensen, Bodil; Bennett, Eric Paul; Andreasson, Erik; Nielsen, Kare L.; Blennow, Andreas; Petersen, Bent Larsen.

In: Scientific Reports, Vol. 9, 17715, 2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Johansen, IE, Liu, Y, Jørgensen, B, Bennett, EP, Andreasson, E, Nielsen, KL, Blennow, A & Petersen, BL 2019, 'High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato', Scientific Reports, vol. 9, 17715. https://doi.org/10.1038/s41598-019-54126-w

APA

Johansen, I. E., Liu, Y., Jørgensen, B., Bennett, E. P., Andreasson, E., Nielsen, K. L., Blennow, A., & Petersen, B. L. (2019). High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato. Scientific Reports, 9, [17715]. https://doi.org/10.1038/s41598-019-54126-w

Vancouver

Johansen IE, Liu Y, Jørgensen B, Bennett EP, Andreasson E, Nielsen KL et al. High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato. Scientific Reports. 2019;9. 17715. https://doi.org/10.1038/s41598-019-54126-w

Author

Johansen, Ida Elisabeth ; Liu, Ying ; Jørgensen, Bodil ; Bennett, Eric Paul ; Andreasson, Erik ; Nielsen, Kare L. ; Blennow, Andreas ; Petersen, Bent Larsen. / High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato. In: Scientific Reports. 2019 ; Vol. 9.

Bibtex

@article{ea8f55aa88394eceb953a7840d8a75c3,
title = "High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato",
abstract = "CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.",
author = "Johansen, {Ida Elisabeth} and Ying Liu and Bodil J{\o}rgensen and Bennett, {Eric Paul} and Erik Andreasson and Nielsen, {Kare L.} and Andreas Blennow and Petersen, {Bent Larsen}",
year = "2019",
doi = "10.1038/s41598-019-54126-w",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

AU - Johansen, Ida Elisabeth

AU - Liu, Ying

AU - Jørgensen, Bodil

AU - Bennett, Eric Paul

AU - Andreasson, Erik

AU - Nielsen, Kare L.

AU - Blennow, Andreas

AU - Petersen, Bent Larsen

PY - 2019

Y1 - 2019

N2 - CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.

AB - CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.

U2 - 10.1038/s41598-019-54126-w

DO - 10.1038/s41598-019-54126-w

M3 - Journal article

C2 - 31776399

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 17715

ER -

ID: 231894257