Genome-wide differential gene expression in children exposed to air pollution in the Czech Republic

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Standard

Genome-wide differential gene expression in children exposed to air pollution in the Czech Republic. / van Leeuwen, D M; van Herwijnen, M H M; Pedersen, Marie; Knudsen, Lisbeth E.; Kirsch-Volders, M; Sram, R J; Staal, Y C M; Bajak, E; van Delft, J H M; Kleinjans, J C S.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 600, No. 1-2, 2006, p. 12-22.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

van Leeuwen, DM, van Herwijnen, MHM, Pedersen, M, Knudsen, LE, Kirsch-Volders, M, Sram, RJ, Staal, YCM, Bajak, E, van Delft, JHM & Kleinjans, JCS 2006, 'Genome-wide differential gene expression in children exposed to air pollution in the Czech Republic', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 600, no. 1-2, pp. 12-22. https://doi.org/10.1016/j.mrfmmm.2006.05.032

APA

van Leeuwen, D. M., van Herwijnen, M. H. M., Pedersen, M., Knudsen, L. E., Kirsch-Volders, M., Sram, R. J., Staal, Y. C. M., Bajak, E., van Delft, J. H. M., & Kleinjans, J. C. S. (2006). Genome-wide differential gene expression in children exposed to air pollution in the Czech Republic. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 600(1-2), 12-22. https://doi.org/10.1016/j.mrfmmm.2006.05.032

Vancouver

van Leeuwen DM, van Herwijnen MHM, Pedersen M, Knudsen LE, Kirsch-Volders M, Sram RJ et al. Genome-wide differential gene expression in children exposed to air pollution in the Czech Republic. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2006;600(1-2):12-22. https://doi.org/10.1016/j.mrfmmm.2006.05.032

Author

van Leeuwen, D M ; van Herwijnen, M H M ; Pedersen, Marie ; Knudsen, Lisbeth E. ; Kirsch-Volders, M ; Sram, R J ; Staal, Y C M ; Bajak, E ; van Delft, J H M ; Kleinjans, J C S. / Genome-wide differential gene expression in children exposed to air pollution in the Czech Republic. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2006 ; Vol. 600, No. 1-2. pp. 12-22.

Bibtex

@article{4d4319400cd311df825d000ea68e967b,

title = "Genome-wide differential gene expression in children exposed to air pollution in the Czech Republic",

abstract = "The Teplice area in the Czech Republic is a mining district where elevated levels of air pollution including airborne carcinogens, have been demonstrated, especially during winter time. This environmental exposure can impact human health; in particular children may be more vulnerable. To study the impact of air pollution in children at the transcriptional level, peripheral blood cells were subjected to whole genome response analysis, in order to identify significantly modulated biological pathways and processes as a result of exposure. Using genome-wide oligonucleotide microarrays, we investigated differential gene expression in children from the Teplice area (n=23) and compared them with children from the rural control area of Prachatice (n=24). In an additional approach, individual gene expressions were correlated with individual peripheral blood lymphocyte micronuclei frequencies, in order to evaluate the linkage of individual gene expressions with an established biomarker of effect that is representative for increased genotoxic risk. Children from the Teplice area showed a significantly higher average micronuclei frequency than Prachatice children (p=0.023). For considerable numbers of genes, the expression differed significantly between the children from the two areas. Amongst these genes, considerable numbers of genes were observed to correlate significantly with the frequencies of micronuclei. The main biological process that appeared significantly affected overall was nucleosome assembly. This suggests an effect of air pollution on the primary structural unit of the condensed DNA. In addition, several other pathways were modulated. Based on the results of this study, we suggest that transcriptomic analysis represents a promising biomarker for environmental carcinogenesis.",

author = "{van Leeuwen}, {D M} and {van Herwijnen}, {M H M} and Marie Pedersen and Knudsen, {Lisbeth E.} and M Kirsch-Volders and Sram, {R J} and Staal, {Y C M} and E Bajak and {van Delft}, {J H M} and Kleinjans, {J C S}",

note = "Keywords: Air Pollutants; Air Pollution; Child; Czech Republic; Environmental Exposure; Female; Gene Expression Regulation; Genomics; Humans; Male; Micronuclei, Chromosome-Defective; Oligonucleotide Array Sequence Analysis",

year = "2006",

doi = "10.1016/j.mrfmmm.2006.05.032",

language = "English",

volume = "600",

pages = "12--22",

journal = "Mutation Research Letters",

issn = "0027-5107",

publisher = "Elsevier",

number = "1-2",

}

RIS

TY - JOUR

T1 - Genome-wide differential gene expression in children exposed to air pollution in the Czech Republic

AU - van Leeuwen, D M

AU - van Herwijnen, M H M

AU - Pedersen, Marie

AU - Knudsen, Lisbeth E.

AU - Kirsch-Volders, M

AU - Sram, R J

AU - Staal, Y C M

AU - Bajak, E

AU - van Delft, J H M

AU - Kleinjans, J C S

N1 - Keywords: Air Pollutants; Air Pollution; Child; Czech Republic; Environmental Exposure; Female; Gene Expression Regulation; Genomics; Humans; Male; Micronuclei, Chromosome-Defective; Oligonucleotide Array Sequence Analysis

PY - 2006

Y1 - 2006

N2 - The Teplice area in the Czech Republic is a mining district where elevated levels of air pollution including airborne carcinogens, have been demonstrated, especially during winter time. This environmental exposure can impact human health; in particular children may be more vulnerable. To study the impact of air pollution in children at the transcriptional level, peripheral blood cells were subjected to whole genome response analysis, in order to identify significantly modulated biological pathways and processes as a result of exposure. Using genome-wide oligonucleotide microarrays, we investigated differential gene expression in children from the Teplice area (n=23) and compared them with children from the rural control area of Prachatice (n=24). In an additional approach, individual gene expressions were correlated with individual peripheral blood lymphocyte micronuclei frequencies, in order to evaluate the linkage of individual gene expressions with an established biomarker of effect that is representative for increased genotoxic risk. Children from the Teplice area showed a significantly higher average micronuclei frequency than Prachatice children (p=0.023). For considerable numbers of genes, the expression differed significantly between the children from the two areas. Amongst these genes, considerable numbers of genes were observed to correlate significantly with the frequencies of micronuclei. The main biological process that appeared significantly affected overall was nucleosome assembly. This suggests an effect of air pollution on the primary structural unit of the condensed DNA. In addition, several other pathways were modulated. Based on the results of this study, we suggest that transcriptomic analysis represents a promising biomarker for environmental carcinogenesis.

AB - The Teplice area in the Czech Republic is a mining district where elevated levels of air pollution including airborne carcinogens, have been demonstrated, especially during winter time. This environmental exposure can impact human health; in particular children may be more vulnerable. To study the impact of air pollution in children at the transcriptional level, peripheral blood cells were subjected to whole genome response analysis, in order to identify significantly modulated biological pathways and processes as a result of exposure. Using genome-wide oligonucleotide microarrays, we investigated differential gene expression in children from the Teplice area (n=23) and compared them with children from the rural control area of Prachatice (n=24). In an additional approach, individual gene expressions were correlated with individual peripheral blood lymphocyte micronuclei frequencies, in order to evaluate the linkage of individual gene expressions with an established biomarker of effect that is representative for increased genotoxic risk. Children from the Teplice area showed a significantly higher average micronuclei frequency than Prachatice children (p=0.023). For considerable numbers of genes, the expression differed significantly between the children from the two areas. Amongst these genes, considerable numbers of genes were observed to correlate significantly with the frequencies of micronuclei. The main biological process that appeared significantly affected overall was nucleosome assembly. This suggests an effect of air pollution on the primary structural unit of the condensed DNA. In addition, several other pathways were modulated. Based on the results of this study, we suggest that transcriptomic analysis represents a promising biomarker for environmental carcinogenesis.

U2 - 10.1016/j.mrfmmm.2006.05.032

DO - 10.1016/j.mrfmmm.2006.05.032

M3 - Journal article

C2 - 16814814

VL - 600

SP - 12

EP - 22

JO - Mutation Research Letters

JF - Mutation Research Letters

SN - 0027-5107

IS - 1-2

ER -

ID: 17273494