Genetic glycoengineering in mammalian cells

Research output: Contribution to journal › Review › Research › peer-review

Standard

Genetic glycoengineering in mammalian cells. / Narimatsu, Yoshiki; Büll, Christian; Chen, Yen Hsi; Wandall, Hans H.; Yang, Zhang; Clausen, Henrik.

In: Journal of Biological Chemistry, Vol. 296, 100448, 2021.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Narimatsu, Y, Büll, C, Chen, YH, Wandall, HH, Yang, Z & Clausen, H 2021, 'Genetic glycoengineering in mammalian cells', Journal of Biological Chemistry, vol. 296, 100448. https://doi.org/10.1016/j.jbc.2021.100448

APA

Narimatsu, Y., Büll, C., Chen, Y. H., Wandall, H. H., Yang, Z., & Clausen, H. (2021). Genetic glycoengineering in mammalian cells. Journal of Biological Chemistry, 296, [100448]. https://doi.org/10.1016/j.jbc.2021.100448

Vancouver

Narimatsu Y, Büll C, Chen YH, Wandall HH, Yang Z, Clausen H. Genetic glycoengineering in mammalian cells. Journal of Biological Chemistry. 2021;296. 100448. https://doi.org/10.1016/j.jbc.2021.100448

Author

Narimatsu, Yoshiki ; Büll, Christian ; Chen, Yen Hsi ; Wandall, Hans H. ; Yang, Zhang ; Clausen, Henrik. / Genetic glycoengineering in mammalian cells. In: Journal of Biological Chemistry. 2021 ; Vol. 296.

Bibtex

@article{04a10f81620d47d58f9c786da6d343a9,
title = "Genetic glycoengineering in mammalian cells",
abstract = "Advances in nuclease-based gene-editing technologies have enabled precise, stable, and systematic genetic engineering of glycosylation capacities in mammalian cells, opening up a plethora of opportunities for studying the glycome and exploiting glycans in biomedicine. Glycoengineering using chemical, enzymatic, and genetic approaches has a long history, and precise gene editing provides a nearly unlimited playground for stable engineering of glycosylation in mammalian cells to explore and dissect the glycome and its many biological functions. Genetic engineering of glycosylation in cells also brings studies of the glycome to the single cell level and opens up wider use and integration of data in traditional omics workflows in cell biology. The last few years have seen new applications of glycoengineering in mammalian cells with perspectives for wider use in basic and applied glycosciences, and these have already led to discoveries of functions of glycans and improved designs of glycoprotein therapeutics. Here, we review the current state of the art of genetic glycoengineering in mammalian cells and highlight emerging opportunities.",
author = "Yoshiki Narimatsu and Christian B{\"u}ll and Chen, {Yen Hsi} and Wandall, {Hans H.} and Zhang Yang and Henrik Clausen",
year = "2021",
doi = "10.1016/j.jbc.2021.100448",
language = "English",
volume = "296",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",

}

RIS

TY - JOUR

T1 - Genetic glycoengineering in mammalian cells

AU - Narimatsu, Yoshiki

AU - Büll, Christian

AU - Chen, Yen Hsi

AU - Wandall, Hans H.

AU - Yang, Zhang

AU - Clausen, Henrik

PY - 2021

Y1 - 2021

N2 - Advances in nuclease-based gene-editing technologies have enabled precise, stable, and systematic genetic engineering of glycosylation capacities in mammalian cells, opening up a plethora of opportunities for studying the glycome and exploiting glycans in biomedicine. Glycoengineering using chemical, enzymatic, and genetic approaches has a long history, and precise gene editing provides a nearly unlimited playground for stable engineering of glycosylation in mammalian cells to explore and dissect the glycome and its many biological functions. Genetic engineering of glycosylation in cells also brings studies of the glycome to the single cell level and opens up wider use and integration of data in traditional omics workflows in cell biology. The last few years have seen new applications of glycoengineering in mammalian cells with perspectives for wider use in basic and applied glycosciences, and these have already led to discoveries of functions of glycans and improved designs of glycoprotein therapeutics. Here, we review the current state of the art of genetic glycoengineering in mammalian cells and highlight emerging opportunities.

AB - Advances in nuclease-based gene-editing technologies have enabled precise, stable, and systematic genetic engineering of glycosylation capacities in mammalian cells, opening up a plethora of opportunities for studying the glycome and exploiting glycans in biomedicine. Glycoengineering using chemical, enzymatic, and genetic approaches has a long history, and precise gene editing provides a nearly unlimited playground for stable engineering of glycosylation in mammalian cells to explore and dissect the glycome and its many biological functions. Genetic engineering of glycosylation in cells also brings studies of the glycome to the single cell level and opens up wider use and integration of data in traditional omics workflows in cell biology. The last few years have seen new applications of glycoengineering in mammalian cells with perspectives for wider use in basic and applied glycosciences, and these have already led to discoveries of functions of glycans and improved designs of glycoprotein therapeutics. Here, we review the current state of the art of genetic glycoengineering in mammalian cells and highlight emerging opportunities.

U2 - 10.1016/j.jbc.2021.100448

DO - 10.1016/j.jbc.2021.100448

M3 - Review

C2 - 33617880

AN - SCOPUS:85103669738

VL - 296

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

M1 - 100448

ER -

ID: 259879499