Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM)

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM). / Xiong, Kai; Zhou, Yan; Hyttel, Poul; Bolund, Lars; Freude, Kristine; Luo, Yonglun.

In: Stem Cell Research, Vol. 17, No. 3, 11.2016, p. 665-669.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Xiong, K, Zhou, Y, Hyttel, P, Bolund, L, Freude, K & Luo, Y 2016, 'Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM)', Stem Cell Research, vol. 17, no. 3, pp. 665-669. https://doi.org/10.1016/j.scr.2016.10.011

APA

Xiong, K., Zhou, Y., Hyttel, P., Bolund, L., Freude, K., & Luo, Y. (2016). Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM). Stem Cell Research, 17(3), 665-669. https://doi.org/10.1016/j.scr.2016.10.011

Vancouver

Xiong K, Zhou Y, Hyttel P, Bolund L, Freude K, Luo Y. Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM). Stem Cell Research. 2016 Nov;17(3):665-669. https://doi.org/10.1016/j.scr.2016.10.011

Author

Xiong, Kai ; Zhou, Yan ; Hyttel, Poul ; Bolund, Lars ; Freude, Kristine ; Luo, Yonglun. / Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM). In: Stem Cell Research. 2016 ; Vol. 17, No. 3. pp. 665-669.

Bibtex

@article{519138d0655c4fd08a44dd6e3ea6610f,
title = "Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM)",
abstract = "Human fibroblasts were engineered to express the CRISPR-based synergistic activation mediator (SAM) complex: dCas9-VP64 and MS2-P65-HSF1. Two induced pluripotent stem cells (iPSCs) clones expressing SAM were established by transducing these fibroblasts with lentivirus expressing OCT4, SOX2, KLF4 and C-MYC. We have validated that the reprogramming cassette is silenced in the SAM iPSC clones. Expression of pluripotency genes (OCT4, SOX2, LIN28A, NANOG, GDF3, SSEA4, and TRA-1-60), differentiation potential to all three germ layers, and normal karyotypes are validated. These SAM-iPSCs provide a novel, useful tool to investigate genetic regulation of stem cell proliferation and differentiation through CRISPR-mediated activation of endogenous genes.",
author = "Kai Xiong and Yan Zhou and Poul Hyttel and Lars Bolund and Kristine Freude and Yonglun Luo",
note = "Copyright {\textcopyright} 2016 The Authors. Published by Elsevier B.V. All rights reserved.",
year = "2016",
month = nov,
doi = "10.1016/j.scr.2016.10.011",
language = "English",
volume = "17",
pages = "665--669",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM)

AU - Xiong, Kai

AU - Zhou, Yan

AU - Hyttel, Poul

AU - Bolund, Lars

AU - Freude, Kristine

AU - Luo, Yonglun

N1 - Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

PY - 2016/11

Y1 - 2016/11

N2 - Human fibroblasts were engineered to express the CRISPR-based synergistic activation mediator (SAM) complex: dCas9-VP64 and MS2-P65-HSF1. Two induced pluripotent stem cells (iPSCs) clones expressing SAM were established by transducing these fibroblasts with lentivirus expressing OCT4, SOX2, KLF4 and C-MYC. We have validated that the reprogramming cassette is silenced in the SAM iPSC clones. Expression of pluripotency genes (OCT4, SOX2, LIN28A, NANOG, GDF3, SSEA4, and TRA-1-60), differentiation potential to all three germ layers, and normal karyotypes are validated. These SAM-iPSCs provide a novel, useful tool to investigate genetic regulation of stem cell proliferation and differentiation through CRISPR-mediated activation of endogenous genes.

AB - Human fibroblasts were engineered to express the CRISPR-based synergistic activation mediator (SAM) complex: dCas9-VP64 and MS2-P65-HSF1. Two induced pluripotent stem cells (iPSCs) clones expressing SAM were established by transducing these fibroblasts with lentivirus expressing OCT4, SOX2, KLF4 and C-MYC. We have validated that the reprogramming cassette is silenced in the SAM iPSC clones. Expression of pluripotency genes (OCT4, SOX2, LIN28A, NANOG, GDF3, SSEA4, and TRA-1-60), differentiation potential to all three germ layers, and normal karyotypes are validated. These SAM-iPSCs provide a novel, useful tool to investigate genetic regulation of stem cell proliferation and differentiation through CRISPR-mediated activation of endogenous genes.

U2 - 10.1016/j.scr.2016.10.011

DO - 10.1016/j.scr.2016.10.011

M3 - Journal article

C2 - 27934604

VL - 17

SP - 665

EP - 669

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 3

ER -

ID: 172429688