Gastric mucosal interleukin-8 and IL-8 antibody concentrations related to prevailing Helicobacter pylori infections: A Danish-Albanian study

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  • Nielsen, Ole Haagen
  • Thomas Horn
  • Skerdi Prifti
  • Peter Peichl
  • Jens Henrik Scheibel
  • Ivan James Dalton Lindley

Objectives: Helicobacter pylori (H. pylori) appears to initiate an inflammatory cascade. Thus, phagocytes are accumulated in the gastric mucosa in inflammatory conditions. Further, a potent chemotactic mediator, interleukin 8 (IL-8) is synthesized at such sites. The recently described IL- 8 autoantibodies may, however, counteract the pro-inflammatory actions of IL- 8. The aim was to study the correlation between H. pylori infection and IL- 8, together with IL-8 autoantibodies in two different populations from a developed and a developing country. Methods: Two different endoscopically characterized populations (65 Danes and 89 Albanians) were examined. IL-8 and IL-8 autoantibodies were detected by ELISA techniques, and H. pylori was identified by histological examinations. Results: Significantly more Albanian controls and dyspeptic patients (80 out of 89 persons) were H. pylori positive as compared to 24 of 65 Danes (p<0.001). The median IL-8 level among Albanian controls 349 pg/mg protein was significantly higher than among Danes <61 pg/mg protein (p<0.001), (p and as at danish found in level patients peptic same the ulcer was>0.05). Further, H. pylori positive patients from both countries had significantly higher levels of IL-8 as compared to H. pylori negative patients (p<0.001). However, significantly higher levels of IL-8 autoantibodies were found in the Albanian sub-population (median 138 O.D. units versus 52 O.D. units among Danes) (p<0.001). Conclusions: In H. pylori related disorders, a high mucosal IL-8 production has been found. However, this investigation further demonstrates higher levels of IL-8 autoantibodies among dyspeptic patients from a developing country, which might possibly counteract the pro-inflammatory actions of IL-8 by binding the molecule. The physiological significance of an altered immune response as described here needs to be elucidated in future studies.

Original languageEnglish
JournalDanish Medical Bulletin
Volume46
Issue number3
Pages (from-to)249-252
Number of pages4
ISSN0907-8916
Publication statusPublished - 1 Jun 1999

ID: 218717509