Gastric mucosal electrical potential difference, pH, blood flow, and morphology during hypoxia and selective gastric ischaemia with and without allopurinol pretreatment in anaesthetized dogs
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Gastric mucosal electrical potential difference, pH, blood flow, and morphology during hypoxia and selective gastric ischaemia with and without allopurinol pretreatment in anaesthetized dogs. / Højgaard, L; Bülow, J B; Madsen, J.
In: Acta Physiologica Scandinavica, Vol. 139, No. 1, 05.1990, p. 185-92.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Gastric mucosal electrical potential difference, pH, blood flow, and morphology during hypoxia and selective gastric ischaemia with and without allopurinol pretreatment in anaesthetized dogs
AU - Højgaard, L
AU - Bülow, J B
AU - Madsen, J
PY - 1990/5
Y1 - 1990/5
N2 - Ischaemia has been implicated in the pathogenesis of gastric mucosal disorders. The aim of this investigation was to study the gastric mucosal electrical potential difference (PD), pH, blood flow and morphology during hypoxia, gastric ischaemia, and gastric ischaemia following inhibition of free radical formation with allopurinol. PD and pH were measured simultaneously with an intragastric microelectrode, and the PD values were corrected for the liquid junction potentials created by the intragastric pH variation. Blood flow was measured by the radiolabelled microsphere technique in 18 anaesthetized dogs. Short general hypoxia and short ischaemia caused reversible declines in PD, increases in pH, and no morphological damage. Ischaemia for 1 h caused a significant decline in PD persistent after reperfusion, an increase in pH, and morphological PD, but after reperfusion PD was normalized. Gross morphology was normal, and histology showed only minor damage. The results therefore support the hypothesis that oxygen-derived free radicals are involved in gastric mucosal injury occurring during ischaemia and reperfusion.
AB - Ischaemia has been implicated in the pathogenesis of gastric mucosal disorders. The aim of this investigation was to study the gastric mucosal electrical potential difference (PD), pH, blood flow and morphology during hypoxia, gastric ischaemia, and gastric ischaemia following inhibition of free radical formation with allopurinol. PD and pH were measured simultaneously with an intragastric microelectrode, and the PD values were corrected for the liquid junction potentials created by the intragastric pH variation. Blood flow was measured by the radiolabelled microsphere technique in 18 anaesthetized dogs. Short general hypoxia and short ischaemia caused reversible declines in PD, increases in pH, and no morphological damage. Ischaemia for 1 h caused a significant decline in PD persistent after reperfusion, an increase in pH, and morphological PD, but after reperfusion PD was normalized. Gross morphology was normal, and histology showed only minor damage. The results therefore support the hypothesis that oxygen-derived free radicals are involved in gastric mucosal injury occurring during ischaemia and reperfusion.
KW - Allopurinol
KW - Animals
KW - Anoxia
KW - Dogs
KW - Electrophysiology
KW - Female
KW - Gastric Mucosa
KW - Hydrogen
KW - Hydrogen-Ion Concentration
KW - Ischemia
KW - Male
KW - Regional Blood Flow
KW - Stomach
KW - Time Factors
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1111/j.1748-1716.1990.tb08911.x
DO - 10.1111/j.1748-1716.1990.tb08911.x
M3 - Journal article
C2 - 2356748
VL - 139
SP - 185
EP - 192
JO - Acta Physiologica Scandinavica
JF - Acta Physiologica Scandinavica
SN - 0001-6772
IS - 1
ER -
ID: 165890979