Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro

Research output: Contribution to journal › Journal article › Research › peer-review

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Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro. / Armer, Hannah; Moffat, Katy; Wileman, Thomas; Belsham, Graham J; Jackson, Terry; Duprex, W Paul; Ryan, Martin; Monaghan, Paul.

In: Journal of Virology, Vol. 82, No. 21, 11.2008, p. 10556-66.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Armer, H, Moffat, K, Wileman, T, Belsham, GJ, Jackson, T, Duprex, WP, Ryan, M & Monaghan, P 2008, 'Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro', Journal of Virology, vol. 82, no. 21, pp. 10556-66. https://doi.org/10.1128/JVI.00907-08

APA

Armer, H., Moffat, K., Wileman, T., Belsham, G. J., Jackson, T., Duprex, W. P., Ryan, M., & Monaghan, P. (2008). Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro. Journal of Virology, 82(21), 10556-66. https://doi.org/10.1128/JVI.00907-08

Vancouver

Armer H, Moffat K, Wileman T, Belsham GJ, Jackson T, Duprex WP et al. Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro. Journal of Virology. 2008 Nov;82(21):10556-66. https://doi.org/10.1128/JVI.00907-08

Author

Armer, Hannah ; Moffat, Katy ; Wileman, Thomas ; Belsham, Graham J ; Jackson, Terry ; Duprex, W Paul ; Ryan, Martin ; Monaghan, Paul. / Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro. In: Journal of Virology. 2008 ; Vol. 82, No. 21. pp. 10556-66.

Bibtex

@article{3c75e036e09a46d1a6c94dd80cfa2ba6,
title = "Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro",
abstract = "Foot-and-mouth disease virus (FMDV), a member of the Picornaviridae, is a pathogen of cloven-hoofed animals and causes a disease of major economic importance. Picornavirus-infected cells show changes in cell morphology and rearrangement of cytoplasmic membranes, which are a consequence of virus replication. We show here, by confocal immunofluorescence and electron microscopy, that the changes in morphology of FMDV-infected cells involve changes in the distribution of microtubule and intermediate filament components during infection. Despite the continued presence of centrosomes in infected cells, there is a loss of tethering of microtubules to the microtubule organizing center (MTOC) region. Loss of labeling for gamma-tubulin, but not pericentrin, from the MTOC suggests a targeting of gamma-tubulin (or associated proteins) rather than a total breakdown in MTOC structure. The identity of the FMDV protein(s) responsible was determined by the expression of individual viral nonstructural proteins and their precursors in uninfected cells. We report that the only viral nonstructural protein able to reproduce the loss of gamma-tubulin from the MTOC and the loss of integrity of the microtubule system is FMDV 3C(pro). In contrast, infection of cells with another picornavirus, bovine enterovirus, did not affect gamma-tubulin distribution, and the microtubule network remained relatively unaffected.",
keywords = "Animals, Cysteine Endopeptidases/metabolism, Cytoskeleton/metabolism, Enterovirus, Bovine/physiology, Foot-and-Mouth Disease Virus/physiology, Microscopy, Confocal, Microscopy, Electron, Microscopy, Fluorescence, Tubulin/metabolism, Viral Proteins/metabolism",
author = "Hannah Armer and Katy Moffat and Thomas Wileman and Belsham, {Graham J} and Terry Jackson and Duprex, {W Paul} and Martin Ryan and Paul Monaghan",
year = "2008",
month = nov,
doi = "10.1128/JVI.00907-08",
language = "English",
volume = "82",
pages = "10556--66",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "21",

}

RIS

TY - JOUR

T1 - Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro

AU - Armer, Hannah

AU - Moffat, Katy

AU - Wileman, Thomas

AU - Belsham, Graham J

AU - Jackson, Terry

AU - Duprex, W Paul

AU - Ryan, Martin

AU - Monaghan, Paul

PY - 2008/11

Y1 - 2008/11

N2 - Foot-and-mouth disease virus (FMDV), a member of the Picornaviridae, is a pathogen of cloven-hoofed animals and causes a disease of major economic importance. Picornavirus-infected cells show changes in cell morphology and rearrangement of cytoplasmic membranes, which are a consequence of virus replication. We show here, by confocal immunofluorescence and electron microscopy, that the changes in morphology of FMDV-infected cells involve changes in the distribution of microtubule and intermediate filament components during infection. Despite the continued presence of centrosomes in infected cells, there is a loss of tethering of microtubules to the microtubule organizing center (MTOC) region. Loss of labeling for gamma-tubulin, but not pericentrin, from the MTOC suggests a targeting of gamma-tubulin (or associated proteins) rather than a total breakdown in MTOC structure. The identity of the FMDV protein(s) responsible was determined by the expression of individual viral nonstructural proteins and their precursors in uninfected cells. We report that the only viral nonstructural protein able to reproduce the loss of gamma-tubulin from the MTOC and the loss of integrity of the microtubule system is FMDV 3C(pro). In contrast, infection of cells with another picornavirus, bovine enterovirus, did not affect gamma-tubulin distribution, and the microtubule network remained relatively unaffected.

AB - Foot-and-mouth disease virus (FMDV), a member of the Picornaviridae, is a pathogen of cloven-hoofed animals and causes a disease of major economic importance. Picornavirus-infected cells show changes in cell morphology and rearrangement of cytoplasmic membranes, which are a consequence of virus replication. We show here, by confocal immunofluorescence and electron microscopy, that the changes in morphology of FMDV-infected cells involve changes in the distribution of microtubule and intermediate filament components during infection. Despite the continued presence of centrosomes in infected cells, there is a loss of tethering of microtubules to the microtubule organizing center (MTOC) region. Loss of labeling for gamma-tubulin, but not pericentrin, from the MTOC suggests a targeting of gamma-tubulin (or associated proteins) rather than a total breakdown in MTOC structure. The identity of the FMDV protein(s) responsible was determined by the expression of individual viral nonstructural proteins and their precursors in uninfected cells. We report that the only viral nonstructural protein able to reproduce the loss of gamma-tubulin from the MTOC and the loss of integrity of the microtubule system is FMDV 3C(pro). In contrast, infection of cells with another picornavirus, bovine enterovirus, did not affect gamma-tubulin distribution, and the microtubule network remained relatively unaffected.

KW - Animals

KW - Cysteine Endopeptidases/metabolism

KW - Cytoskeleton/metabolism

KW - Enterovirus, Bovine/physiology

KW - Foot-and-Mouth Disease Virus/physiology

KW - Microscopy, Confocal

KW - Microscopy, Electron

KW - Microscopy, Fluorescence

KW - Tubulin/metabolism

KW - Viral Proteins/metabolism

U2 - 10.1128/JVI.00907-08

DO - 10.1128/JVI.00907-08

M3 - Journal article

C2 - 18753210

VL - 82

SP - 10556

EP - 10566

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 21

ER -

ID: 257918308