Flow cytometric detection of wild yeast in lager breweries
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Flow cytometric detection of wild yeast in lager breweries. / Jespersen, Lene; Lassen, S-; Jakobsen, Mogens.
In: International Journal of Food Microbiology, Vol. 17, No. 4, 1993, p. 321-328.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Flow cytometric detection of wild yeast in lager breweries
AU - Jespersen, Lene
AU - Lassen, S-
AU - Jakobsen, Mogens
PY - 1993
Y1 - 1993
N2 - A flow cytometric method for detection of wild yeast infections in breweries is reported. It is based on selective enrichment in Malt extract Yeast extract Glucose Peptone broth (MYGP) at 37 degrees C and in MYGP with 200 ppm CuSO4 at 25 degrees C, staining with a fluorochrome precursor and flow cytometry. In experiments with several types of wild yeast isolated from breweries and two different strains of lager yeast it has been possible to detect one wild yeast per 10(6) culture yeast after 48-72 h of incubation and, in some cases, after 24 h.
AB - A flow cytometric method for detection of wild yeast infections in breweries is reported. It is based on selective enrichment in Malt extract Yeast extract Glucose Peptone broth (MYGP) at 37 degrees C and in MYGP with 200 ppm CuSO4 at 25 degrees C, staining with a fluorochrome precursor and flow cytometry. In experiments with several types of wild yeast isolated from breweries and two different strains of lager yeast it has been possible to detect one wild yeast per 10(6) culture yeast after 48-72 h of incubation and, in some cases, after 24 h.
KW - Beer
KW - Evaluation Studies as Topic
KW - Flow Cytometry
KW - Food Microbiology
KW - Mycology
KW - Pichia
KW - Saccharomyces cerevisiae
U2 - 10.1016/0168-1605(93)90202-R
DO - 10.1016/0168-1605(93)90202-R
M3 - Journal article
C2 - 8466805
VL - 17
SP - 321
EP - 328
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
SN - 0168-1605
IS - 4
ER -
ID: 131532094