Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. / Seuring, Carolin; Ayyer, Kartik; Filippaki, Eleftheria; Barthelmess, Miriam; Longchamp, Jean-Nicolas; Ringler, Philippe; Pardini, Tommaso; Wojtas, David H; Coleman, Matthew A; Dörner, Katerina; Fuglerud, Silje; Hammarin, Greger; Habenstein, Birgit; Langkilde, Annette E; Loquet, Antoine; Meents, Alke; Riek, Roland; Stahlberg, Henning; Boutet, Sébastien; Hunter, Mark S; Koglin, Jason; Liang, Mengning; Ginn, Helen M; Millane, Rick P; Frank, Matthias; Barty, Anton; Chapman, Henry N.

In: Nature Communications, Vol. 9, No. 1, 1836, 09.05.2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Seuring, C, Ayyer, K, Filippaki, E, Barthelmess, M, Longchamp, J-N, Ringler, P, Pardini, T, Wojtas, DH, Coleman, MA, Dörner, K, Fuglerud, S, Hammarin, G, Habenstein, B, Langkilde, AE, Loquet, A, Meents, A, Riek, R, Stahlberg, H, Boutet, S, Hunter, MS, Koglin, J, Liang, M, Ginn, HM, Millane, RP, Frank, M, Barty, A & Chapman, HN 2018, 'Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene', Nature Communications, vol. 9, no. 1, 1836. https://doi.org/10.1038/s41467-018-04116-9

APA

Seuring, C., Ayyer, K., Filippaki, E., Barthelmess, M., Longchamp, J-N., Ringler, P., Pardini, T., Wojtas, D. H., Coleman, M. A., Dörner, K., Fuglerud, S., Hammarin, G., Habenstein, B., Langkilde, A. E., Loquet, A., Meents, A., Riek, R., Stahlberg, H., Boutet, S., ... Chapman, H. N. (2018). Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. Nature Communications, 9(1), [1836]. https://doi.org/10.1038/s41467-018-04116-9

Vancouver

Seuring C, Ayyer K, Filippaki E, Barthelmess M, Longchamp J-N, Ringler P et al. Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. Nature Communications. 2018 May 9;9(1). 1836. https://doi.org/10.1038/s41467-018-04116-9

Author

Seuring, Carolin ; Ayyer, Kartik ; Filippaki, Eleftheria ; Barthelmess, Miriam ; Longchamp, Jean-Nicolas ; Ringler, Philippe ; Pardini, Tommaso ; Wojtas, David H ; Coleman, Matthew A ; Dörner, Katerina ; Fuglerud, Silje ; Hammarin, Greger ; Habenstein, Birgit ; Langkilde, Annette E ; Loquet, Antoine ; Meents, Alke ; Riek, Roland ; Stahlberg, Henning ; Boutet, Sébastien ; Hunter, Mark S ; Koglin, Jason ; Liang, Mengning ; Ginn, Helen M ; Millane, Rick P ; Frank, Matthias ; Barty, Anton ; Chapman, Henry N. / Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. In: Nature Communications. 2018 ; Vol. 9, No. 1.

Bibtex

@article{da37067109a04d77960acc704a459e4e,
title = "Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene",
abstract = "Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 {\AA} and 2.4 {\AA} resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.",
author = "Carolin Seuring and Kartik Ayyer and Eleftheria Filippaki and Miriam Barthelmess and Jean-Nicolas Longchamp and Philippe Ringler and Tommaso Pardini and Wojtas, {David H} and Coleman, {Matthew A} and Katerina D{\"o}rner and Silje Fuglerud and Greger Hammarin and Birgit Habenstein and Langkilde, {Annette E} and Antoine Loquet and Alke Meents and Roland Riek and Henning Stahlberg and S{\'e}bastien Boutet and Hunter, {Mark S} and Jason Koglin and Mengning Liang and Ginn, {Helen M} and Millane, {Rick P} and Matthias Frank and Anton Barty and Chapman, {Henry N}",
year = "2018",
month = may,
day = "9",
doi = "10.1038/s41467-018-04116-9",
language = "English",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

AU - Seuring, Carolin

AU - Ayyer, Kartik

AU - Filippaki, Eleftheria

AU - Barthelmess, Miriam

AU - Longchamp, Jean-Nicolas

AU - Ringler, Philippe

AU - Pardini, Tommaso

AU - Wojtas, David H

AU - Coleman, Matthew A

AU - Dörner, Katerina

AU - Fuglerud, Silje

AU - Hammarin, Greger

AU - Habenstein, Birgit

AU - Langkilde, Annette E

AU - Loquet, Antoine

AU - Meents, Alke

AU - Riek, Roland

AU - Stahlberg, Henning

AU - Boutet, Sébastien

AU - Hunter, Mark S

AU - Koglin, Jason

AU - Liang, Mengning

AU - Ginn, Helen M

AU - Millane, Rick P

AU - Frank, Matthias

AU - Barty, Anton

AU - Chapman, Henry N

PY - 2018/5/9

Y1 - 2018/5/9

N2 - Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

AB - Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

U2 - 10.1038/s41467-018-04116-9

DO - 10.1038/s41467-018-04116-9

M3 - Journal article

C2 - 29743480

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1836

ER -

ID: 196343097