Expression of Na+/HCO3- co-transporter proteins (NBCs) in rat and human skeletal muscle

Research output: Contribution to journalJournal articleResearchpeer-review

  • Jonas Møller Kristensen
  • Michael Kristensen
  • Carsten Juel
AIM: Sodium/bicarbonate co-transport (NBC) has been suggested to have a role in muscle pH regulation. We investigated the presence of NBC proteins in rat and human muscle samples and the fibre type distribution of the identified NBCs. METHODS AND RESULTS: Western blotting of muscle homogenates and sarcolemmal membranes (sarcolemmal giant vesicles) were used to screen for the presence of NBCs. Immunohistochemistry was used for the subcellular localization. The functional test revealed that approximately half of the pH recovery in sarcolemmal vesicles produced from rat muscle is mediated by bicarbonate-dependent transport. This indicates that the NBCs are preserved in the vesicles. The western blotting experiments demonstrated the existence of at least two NBC proteins in skeletal muscle. One NBC protein (approximately 150 kDa) seems to be related to the kidney/pancreas/heart isoform NBC1, whereas the other protein (approximately 200 kDa) is related to the NBC4 isoform. The two NBC proteins represent the electrogenic isoforms named NBCe1 and NBCe2. Membrane fractionation and immunofluorescence techniques confirmed that the two NBCs are located in the sarcolemmal membrane as well as in some internal membranes, probably the T-tubules. The two NBCs localized in muscle have distinct fibre type distributions. CONCLUSIONS: Skeletal muscle possesses two variants of the sodium/bicarbonate co-transporter (NBC) isoforms, which have been called NBCe1 and NBCe2.
Original languageEnglish
JournalActa Physiologica (Print Edition)
Issue number1
Pages (from-to)69-76
Number of pages8
Publication statusPublished - 2004

Bibliographical note

Keywords: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Animals; Bicarbonates; Blotting, Western; Humans; Immunohistochemistry; Microscopy, Fluorescence; Muscle Fibers; Muscle, Skeletal; Protein Isoforms; Rats; Sarcolemma; Sodium-Bicarbonate Symporters

ID: 8853829