Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation

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Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation. / Simonsen, Stine; Thyssen, Jacob P.; Heegaard, Steffen; Kezic, Sanja; Skov, Lone.

In: Acta Dermato-Venereologica, Vol. 97, No. 7, 2017, p. 797-801.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Simonsen, S, Thyssen, JP, Heegaard, S, Kezic, S & Skov, L 2017, 'Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation', Acta Dermato-Venereologica, vol. 97, no. 7, pp. 797-801. https://doi.org/10.2340/00015555-2662

APA

Simonsen, S., Thyssen, J. P., Heegaard, S., Kezic, S., & Skov, L. (2017). Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation. Acta Dermato-Venereologica, 97(7), 797-801. https://doi.org/10.2340/00015555-2662

Vancouver

Simonsen S, Thyssen JP, Heegaard S, Kezic S, Skov L. Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation. Acta Dermato-Venereologica. 2017;97(7):797-801. https://doi.org/10.2340/00015555-2662

Author

Simonsen, Stine ; Thyssen, Jacob P. ; Heegaard, Steffen ; Kezic, Sanja ; Skov, Lone. / Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation. In: Acta Dermato-Venereologica. 2017 ; Vol. 97, No. 7. pp. 797-801.

Bibtex

@article{1dc1a12c9a884c17ab72053f3b21a196,
title = "Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation",
abstract = "Epidermal filaggrin level is affected by ultraviolet B irradiation in animal and experimental models. This study examined the effect of ultraviolet B irradiation on epidermal filaggrin and natural moisturizing factors in vivo in healthy adults (n = 22). Participants were irradiated with 2 minimal erythema doses of ultraviolet B on the skin. Biopsies and tape strips were collected from skin irradiated 24 and 72 h earlier and from nonirradiated skin (control). Real-time quantitative PCR on skin biopsies showed significantly reduced profilaggrin mRNA expression 24 h after irradiation (mean relative mRNA expression ± standard deviation: control, 3.86 ± 2.06 vs. 24 h, 1.52 ± 0.640; p = 0.02; n = 8). Immunohistochemistry showed aberrant spatial distribution of filaggrin protein 72 h after irradiation (n = 3). High-pressure liquid chromatography of tape extracts showed no change in mean total natural moisturizing factor levels after irradiation, but mean trans-urocanic acid was significantly reduced, as expected (n = 8). In conclusion, erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo.",
keywords = "Acute inflammation, Filaggrin, Human, Ultraviolet B",
author = "Stine Simonsen and Thyssen, {Jacob P.} and Steffen Heegaard and Sanja Kezic and Lone Skov",
year = "2017",
doi = "10.2340/00015555-2662",
language = "English",
volume = "97",
pages = "797--801",
journal = "Acta Dermatovenereologica",
issn = "0001-5555",
publisher = "Society for the Publication of Acta Dermato - Venereologica",
number = "7",

}

RIS

TY - JOUR

T1 - Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation

AU - Simonsen, Stine

AU - Thyssen, Jacob P.

AU - Heegaard, Steffen

AU - Kezic, Sanja

AU - Skov, Lone

PY - 2017

Y1 - 2017

N2 - Epidermal filaggrin level is affected by ultraviolet B irradiation in animal and experimental models. This study examined the effect of ultraviolet B irradiation on epidermal filaggrin and natural moisturizing factors in vivo in healthy adults (n = 22). Participants were irradiated with 2 minimal erythema doses of ultraviolet B on the skin. Biopsies and tape strips were collected from skin irradiated 24 and 72 h earlier and from nonirradiated skin (control). Real-time quantitative PCR on skin biopsies showed significantly reduced profilaggrin mRNA expression 24 h after irradiation (mean relative mRNA expression ± standard deviation: control, 3.86 ± 2.06 vs. 24 h, 1.52 ± 0.640; p = 0.02; n = 8). Immunohistochemistry showed aberrant spatial distribution of filaggrin protein 72 h after irradiation (n = 3). High-pressure liquid chromatography of tape extracts showed no change in mean total natural moisturizing factor levels after irradiation, but mean trans-urocanic acid was significantly reduced, as expected (n = 8). In conclusion, erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo.

AB - Epidermal filaggrin level is affected by ultraviolet B irradiation in animal and experimental models. This study examined the effect of ultraviolet B irradiation on epidermal filaggrin and natural moisturizing factors in vivo in healthy adults (n = 22). Participants were irradiated with 2 minimal erythema doses of ultraviolet B on the skin. Biopsies and tape strips were collected from skin irradiated 24 and 72 h earlier and from nonirradiated skin (control). Real-time quantitative PCR on skin biopsies showed significantly reduced profilaggrin mRNA expression 24 h after irradiation (mean relative mRNA expression ± standard deviation: control, 3.86 ± 2.06 vs. 24 h, 1.52 ± 0.640; p = 0.02; n = 8). Immunohistochemistry showed aberrant spatial distribution of filaggrin protein 72 h after irradiation (n = 3). High-pressure liquid chromatography of tape extracts showed no change in mean total natural moisturizing factor levels after irradiation, but mean trans-urocanic acid was significantly reduced, as expected (n = 8). In conclusion, erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo.

KW - Acute inflammation

KW - Filaggrin

KW - Human

KW - Ultraviolet B

U2 - 10.2340/00015555-2662

DO - 10.2340/00015555-2662

M3 - Journal article

C2 - 28358172

AN - SCOPUS:85021887138

VL - 97

SP - 797

EP - 801

JO - Acta Dermatovenereologica

JF - Acta Dermatovenereologica

SN - 0001-5555

IS - 7

ER -

ID: 182093142