Evaluation of the effects of spray drying parameters for producing cubosome powder precursors

Research output: Contribution to journalJournal articlepeer-review

Standard

Evaluation of the effects of spray drying parameters for producing cubosome powder precursors. / von Halling Laier, Christoffer; Sonne Alstrøm, Tommy; Travers Bargholz, Mia; Bjerg Sjøltov, Pernille; Rades, Thomas; Boisen, Anja; Nielsen, Line Hagner.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 135, 2019, p. 44-48.

Research output: Contribution to journalJournal articlepeer-review

Harvard

von Halling Laier, C, Sonne Alstrøm, T, Travers Bargholz, M, Bjerg Sjøltov, P, Rades, T, Boisen, A & Nielsen, LH 2019, 'Evaluation of the effects of spray drying parameters for producing cubosome powder precursors', European Journal of Pharmaceutics and Biopharmaceutics, vol. 135, pp. 44-48. https://doi.org/10.1016/j.ejpb.2018.12.008

APA

von Halling Laier, C., Sonne Alstrøm, T., Travers Bargholz, M., Bjerg Sjøltov, P., Rades, T., Boisen, A., & Nielsen, L. H. (2019). Evaluation of the effects of spray drying parameters for producing cubosome powder precursors. European Journal of Pharmaceutics and Biopharmaceutics, 135, 44-48. https://doi.org/10.1016/j.ejpb.2018.12.008

Vancouver

von Halling Laier C, Sonne Alstrøm T, Travers Bargholz M, Bjerg Sjøltov P, Rades T, Boisen A et al. Evaluation of the effects of spray drying parameters for producing cubosome powder precursors. European Journal of Pharmaceutics and Biopharmaceutics. 2019;135:44-48. https://doi.org/10.1016/j.ejpb.2018.12.008

Author

von Halling Laier, Christoffer ; Sonne Alstrøm, Tommy ; Travers Bargholz, Mia ; Bjerg Sjøltov, Pernille ; Rades, Thomas ; Boisen, Anja ; Nielsen, Line Hagner. / Evaluation of the effects of spray drying parameters for producing cubosome powder precursors. In: European Journal of Pharmaceutics and Biopharmaceutics. 2019 ; Vol. 135. pp. 44-48.

Bibtex

@article{568653fc13fa4ff2a692233b234765f8,
title = "Evaluation of the effects of spray drying parameters for producing cubosome powder precursors",
abstract = "Vaccines provide great benefit for global health, but are insufficiently distributed in developing countries due to high costs of manufacturing and limited storage stability. Spray drying formulations of peptide-based vaccines offer a promising strategy to reduce production costs and improve unrefrigerated storage stability. This design of experiments investigated how adjusting spray drying parameters (inlet temperature, atomization pressure, feed rate and aspiration rate) affects residual moisture and reconstitution properties of the powder product, and morphology and size of the rehydrated particles. An emulsion capable of forming cubosomes with the protein ovalbumin as model antigen was used as vaccine formulation and spray dried. The produced powders had low residual moisture content, independent of the spray drying parameters within the range investigated. Cryogenic transmission electron microscopy revealed that cubosomes were produced after rehydration regardless of the spray drying parameters. Furthermore, dynamic light scattering showed that the average diameter of the rehydrated particles consistently was close to 230 nm at any combination of spray drying parameters as long as the outlet temperature was kept below a threshold of 115 °C. The process thus appears to be robust allowing optimization to be focused on yield, dry particle engineering or outlet temperature considerations.",
keywords = "Colloids, Design of experiments, Monoolein, Ovalbumin, Particles, Vaccine",
author = "{von Halling Laier}, Christoffer and {Sonne Alstr{\o}m}, Tommy and {Travers Bargholz}, Mia and {Bjerg Sj{\o}ltov}, Pernille and Thomas Rades and Anja Boisen and Nielsen, {Line Hagner}",
year = "2019",
doi = "10.1016/j.ejpb.2018.12.008",
language = "English",
volume = "135",
pages = "44--48",
journal = "European Journal of Pharmaceutics and Biopharmaceutics",
issn = "0939-6411",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Evaluation of the effects of spray drying parameters for producing cubosome powder precursors

AU - von Halling Laier, Christoffer

AU - Sonne Alstrøm, Tommy

AU - Travers Bargholz, Mia

AU - Bjerg Sjøltov, Pernille

AU - Rades, Thomas

AU - Boisen, Anja

AU - Nielsen, Line Hagner

PY - 2019

Y1 - 2019

N2 - Vaccines provide great benefit for global health, but are insufficiently distributed in developing countries due to high costs of manufacturing and limited storage stability. Spray drying formulations of peptide-based vaccines offer a promising strategy to reduce production costs and improve unrefrigerated storage stability. This design of experiments investigated how adjusting spray drying parameters (inlet temperature, atomization pressure, feed rate and aspiration rate) affects residual moisture and reconstitution properties of the powder product, and morphology and size of the rehydrated particles. An emulsion capable of forming cubosomes with the protein ovalbumin as model antigen was used as vaccine formulation and spray dried. The produced powders had low residual moisture content, independent of the spray drying parameters within the range investigated. Cryogenic transmission electron microscopy revealed that cubosomes were produced after rehydration regardless of the spray drying parameters. Furthermore, dynamic light scattering showed that the average diameter of the rehydrated particles consistently was close to 230 nm at any combination of spray drying parameters as long as the outlet temperature was kept below a threshold of 115 °C. The process thus appears to be robust allowing optimization to be focused on yield, dry particle engineering or outlet temperature considerations.

AB - Vaccines provide great benefit for global health, but are insufficiently distributed in developing countries due to high costs of manufacturing and limited storage stability. Spray drying formulations of peptide-based vaccines offer a promising strategy to reduce production costs and improve unrefrigerated storage stability. This design of experiments investigated how adjusting spray drying parameters (inlet temperature, atomization pressure, feed rate and aspiration rate) affects residual moisture and reconstitution properties of the powder product, and morphology and size of the rehydrated particles. An emulsion capable of forming cubosomes with the protein ovalbumin as model antigen was used as vaccine formulation and spray dried. The produced powders had low residual moisture content, independent of the spray drying parameters within the range investigated. Cryogenic transmission electron microscopy revealed that cubosomes were produced after rehydration regardless of the spray drying parameters. Furthermore, dynamic light scattering showed that the average diameter of the rehydrated particles consistently was close to 230 nm at any combination of spray drying parameters as long as the outlet temperature was kept below a threshold of 115 °C. The process thus appears to be robust allowing optimization to be focused on yield, dry particle engineering or outlet temperature considerations.

KW - Colloids

KW - Design of experiments

KW - Monoolein

KW - Ovalbumin

KW - Particles

KW - Vaccine

U2 - 10.1016/j.ejpb.2018.12.008

DO - 10.1016/j.ejpb.2018.12.008

M3 - Journal article

C2 - 30576708

AN - SCOPUS:85058678528

VL - 135

SP - 44

EP - 48

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

ER -

ID: 241098942