Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis

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Standard

Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis. / Elmgreen, J; Nielsen, O H; Ahnfelt-Rønne, I.

In: Annals of the Rheumatic Diseases, Vol. 46, No. 7, 07.1987, p. 501-5.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Elmgreen, J, Nielsen, OH & Ahnfelt-Rønne, I 1987, 'Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis', Annals of the Rheumatic Diseases, vol. 46, no. 7, pp. 501-5. https://doi.org/10.1136/ard.46.7.501

APA

Elmgreen, J., Nielsen, O. H., & Ahnfelt-Rønne, I. (1987). Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis. Annals of the Rheumatic Diseases, 46(7), 501-5. https://doi.org/10.1136/ard.46.7.501

Vancouver

Elmgreen J, Nielsen OH, Ahnfelt-Rønne I. Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis. Annals of the Rheumatic Diseases. 1987 Jul;46(7):501-5. https://doi.org/10.1136/ard.46.7.501

Author

Elmgreen, J ; Nielsen, O H ; Ahnfelt-Rønne, I. / Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis. In: Annals of the Rheumatic Diseases. 1987 ; Vol. 46, No. 7. pp. 501-5.

Bibtex

@article{5da7ff0eab2b42d78b5edd741db7ed87,
title = "Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis",
abstract = "The calcium dependent metabolism of endogenous arachidonic acid (AA) was investigated in 17 patients with rheumatoid arthritis during treatment with dextropropoxyphene alone and in 25 healthy volunteers. Incorporation of [1-14C]AA into intracellular phospholipids of purified neutrophils was achieved by incubation until steady state before activation with ionophore A23187. Analysis of extracellular metabolites was performed by extraction, thin layer chromatography, autoradiography, and laser densitometry. The patients showed a twofold increase in the total capacity for oxidation of AA. Release of leucotriene B4 (LTB4) and its omega oxidation products, 20-OH LTB4 and 20-COOH LTB4, was 29{\%}, range 11-48{\%}, in patients compared with 8{\%}, range 4-12{\%}, in healthy volunteers. Total amounts of radioactivity released and the specific activity of LTB4, as assessed by high pressure liquid chromatography, were equal in experimental and control groups. The demonstrated increased capacity for metabolism of AA to the major proinflammatory metabolite, LTB4, via the 5-lipoxygenase pathway may contribute to perpetuation of inflammation and to tissue destruction in rheumatoid arthritis.",
keywords = "Adult, Aged, Arachidonic Acid, Arachidonic Acids/blood, Arthritis, Rheumatoid/blood, Calcimycin/pharmacology, Chromatography, Thin Layer, Female, Humans, Leukotriene B4/blood, Male, Middle Aged, Neutrophils/analysis",
author = "J Elmgreen and Nielsen, {O H} and I Ahnfelt-R{\o}nne",
year = "1987",
month = "7",
doi = "10.1136/ard.46.7.501",
language = "English",
volume = "46",
pages = "501--5",
journal = "Annals of the Rheumatic Diseases",
issn = "0003-4967",
publisher = "B M J Group",
number = "7",

}

RIS

TY - JOUR

T1 - Enhanced capacity for release of leucotriene B4 by neutrophils in rheumatoid arthritis

AU - Elmgreen, J

AU - Nielsen, O H

AU - Ahnfelt-Rønne, I

PY - 1987/7

Y1 - 1987/7

N2 - The calcium dependent metabolism of endogenous arachidonic acid (AA) was investigated in 17 patients with rheumatoid arthritis during treatment with dextropropoxyphene alone and in 25 healthy volunteers. Incorporation of [1-14C]AA into intracellular phospholipids of purified neutrophils was achieved by incubation until steady state before activation with ionophore A23187. Analysis of extracellular metabolites was performed by extraction, thin layer chromatography, autoradiography, and laser densitometry. The patients showed a twofold increase in the total capacity for oxidation of AA. Release of leucotriene B4 (LTB4) and its omega oxidation products, 20-OH LTB4 and 20-COOH LTB4, was 29%, range 11-48%, in patients compared with 8%, range 4-12%, in healthy volunteers. Total amounts of radioactivity released and the specific activity of LTB4, as assessed by high pressure liquid chromatography, were equal in experimental and control groups. The demonstrated increased capacity for metabolism of AA to the major proinflammatory metabolite, LTB4, via the 5-lipoxygenase pathway may contribute to perpetuation of inflammation and to tissue destruction in rheumatoid arthritis.

AB - The calcium dependent metabolism of endogenous arachidonic acid (AA) was investigated in 17 patients with rheumatoid arthritis during treatment with dextropropoxyphene alone and in 25 healthy volunteers. Incorporation of [1-14C]AA into intracellular phospholipids of purified neutrophils was achieved by incubation until steady state before activation with ionophore A23187. Analysis of extracellular metabolites was performed by extraction, thin layer chromatography, autoradiography, and laser densitometry. The patients showed a twofold increase in the total capacity for oxidation of AA. Release of leucotriene B4 (LTB4) and its omega oxidation products, 20-OH LTB4 and 20-COOH LTB4, was 29%, range 11-48%, in patients compared with 8%, range 4-12%, in healthy volunteers. Total amounts of radioactivity released and the specific activity of LTB4, as assessed by high pressure liquid chromatography, were equal in experimental and control groups. The demonstrated increased capacity for metabolism of AA to the major proinflammatory metabolite, LTB4, via the 5-lipoxygenase pathway may contribute to perpetuation of inflammation and to tissue destruction in rheumatoid arthritis.

KW - Adult

KW - Aged

KW - Arachidonic Acid

KW - Arachidonic Acids/blood

KW - Arthritis, Rheumatoid/blood

KW - Calcimycin/pharmacology

KW - Chromatography, Thin Layer

KW - Female

KW - Humans

KW - Leukotriene B4/blood

KW - Male

KW - Middle Aged

KW - Neutrophils/analysis

U2 - 10.1136/ard.46.7.501

DO - 10.1136/ard.46.7.501

M3 - Journal article

C2 - 2821934

VL - 46

SP - 501

EP - 505

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

SN - 0003-4967

IS - 7

ER -

ID: 218729983