Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish

Research output: Contribution to journalJournal articlepeer-review

Standard

Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish. / Kheawfu, Kantaporn; Pikulkaew, Surachai; Wellendorph, Petrine; Jørgensen, Louise von Gersdorff; Rades, Thomas; Mullertz, Anette ; Okonogi, Siriporn.

In: Pharmaceutics, Vol. 14, No. 5, 919, 2022.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kheawfu, K, Pikulkaew, S, Wellendorph, P, Jørgensen, LVG, Rades, T, Mullertz, A & Okonogi, S 2022, 'Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish', Pharmaceutics, vol. 14, no. 5, 919. https://doi.org/10.3390/pharmaceutics14050919

APA

Kheawfu, K., Pikulkaew, S., Wellendorph, P., Jørgensen, L. V. G., Rades, T., Mullertz, A., & Okonogi, S. (2022). Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish. Pharmaceutics, 14(5), [919]. https://doi.org/10.3390/pharmaceutics14050919

Vancouver

Kheawfu K, Pikulkaew S, Wellendorph P, Jørgensen LVG, Rades T, Mullertz A et al. Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish. Pharmaceutics. 2022;14(5). 919. https://doi.org/10.3390/pharmaceutics14050919

Author

Kheawfu, Kantaporn ; Pikulkaew, Surachai ; Wellendorph, Petrine ; Jørgensen, Louise von Gersdorff ; Rades, Thomas ; Mullertz, Anette ; Okonogi, Siriporn. / Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish. In: Pharmaceutics. 2022 ; Vol. 14, No. 5.

Bibtex

@article{1606ec43e4144cf3abc0d56288c8d1ba,
title = "Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish",
abstract = "Clove oil (CO), an essential oil of Syzygium aromaticum, has been reported as an anesthetic for many fish species. However, its insoluble properties require a suitable delivery system for its application. In the present study, nanoformulations of CO as a nanoemulsion (CO-NE), a self-microemulsifying drug-delivery system (CO-SMEDDS), and a self-nanoemulsifying drug-delivery system (CO-SNEDDS) were prepared for delivering CO. Zebrafish were used as a fish model to investigate oil pathways. The result shows fluorescence spots of fluorescence-labeled CO accumulate on the gills, skin, and brain. All CO nanoformulations significantly increased penetration flux compared to CO ethanolic solution. Investigation of the anesthetic mechanism of action using a rat brain γ-aminobutyric acid subtype A (GABAA) receptor-binding test demonstrates that CO and its major compound, eugenol, modulate [3H]muscimol binding. CO-NE exhibited a concentration-dependent binding activity with an EC50 value of 175 µg/mL, significantly higher than CO solution in dimethyl sulfoxide. In conclusion, CO enters the fish through the skin and gills. The anesthetic mechanism of action of CO is based on modulation of [3H] muscimol binding to GABAA receptors. Among three nanoformulations tested, CO-NE is the most effective at increasing permeability and enhancing the receptor-binding activity of the oil. ",
author = "Kantaporn Kheawfu and Surachai Pikulkaew and Petrine Wellendorph and J{\o}rgensen, {Louise von Gersdorff} and Thomas Rades and Anette Mullertz and Siriporn Okonogi",
note = "This article belongs to the Special Issue Essential Oils in Pharmaceutical Products.",
year = "2022",
doi = "10.3390/pharmaceutics14050919",
language = "English",
volume = "14",
journal = "Pharmaceutics",
issn = "1999-4923",
publisher = "MDPI AG",
number = "5",

}

RIS

TY - JOUR

T1 - Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish

AU - Kheawfu, Kantaporn

AU - Pikulkaew, Surachai

AU - Wellendorph, Petrine

AU - Jørgensen, Louise von Gersdorff

AU - Rades, Thomas

AU - Mullertz, Anette

AU - Okonogi, Siriporn

N1 - This article belongs to the Special Issue Essential Oils in Pharmaceutical Products.

PY - 2022

Y1 - 2022

N2 - Clove oil (CO), an essential oil of Syzygium aromaticum, has been reported as an anesthetic for many fish species. However, its insoluble properties require a suitable delivery system for its application. In the present study, nanoformulations of CO as a nanoemulsion (CO-NE), a self-microemulsifying drug-delivery system (CO-SMEDDS), and a self-nanoemulsifying drug-delivery system (CO-SNEDDS) were prepared for delivering CO. Zebrafish were used as a fish model to investigate oil pathways. The result shows fluorescence spots of fluorescence-labeled CO accumulate on the gills, skin, and brain. All CO nanoformulations significantly increased penetration flux compared to CO ethanolic solution. Investigation of the anesthetic mechanism of action using a rat brain γ-aminobutyric acid subtype A (GABAA) receptor-binding test demonstrates that CO and its major compound, eugenol, modulate [3H]muscimol binding. CO-NE exhibited a concentration-dependent binding activity with an EC50 value of 175 µg/mL, significantly higher than CO solution in dimethyl sulfoxide. In conclusion, CO enters the fish through the skin and gills. The anesthetic mechanism of action of CO is based on modulation of [3H] muscimol binding to GABAA receptors. Among three nanoformulations tested, CO-NE is the most effective at increasing permeability and enhancing the receptor-binding activity of the oil.

AB - Clove oil (CO), an essential oil of Syzygium aromaticum, has been reported as an anesthetic for many fish species. However, its insoluble properties require a suitable delivery system for its application. In the present study, nanoformulations of CO as a nanoemulsion (CO-NE), a self-microemulsifying drug-delivery system (CO-SMEDDS), and a self-nanoemulsifying drug-delivery system (CO-SNEDDS) were prepared for delivering CO. Zebrafish were used as a fish model to investigate oil pathways. The result shows fluorescence spots of fluorescence-labeled CO accumulate on the gills, skin, and brain. All CO nanoformulations significantly increased penetration flux compared to CO ethanolic solution. Investigation of the anesthetic mechanism of action using a rat brain γ-aminobutyric acid subtype A (GABAA) receptor-binding test demonstrates that CO and its major compound, eugenol, modulate [3H]muscimol binding. CO-NE exhibited a concentration-dependent binding activity with an EC50 value of 175 µg/mL, significantly higher than CO solution in dimethyl sulfoxide. In conclusion, CO enters the fish through the skin and gills. The anesthetic mechanism of action of CO is based on modulation of [3H] muscimol binding to GABAA receptors. Among three nanoformulations tested, CO-NE is the most effective at increasing permeability and enhancing the receptor-binding activity of the oil.

U2 - 10.3390/pharmaceutics14050919

DO - 10.3390/pharmaceutics14050919

M3 - Journal article

C2 - 35631505

VL - 14

JO - Pharmaceutics

JF - Pharmaceutics

SN - 1999-4923

IS - 5

M1 - 919

ER -

ID: 305008982