Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex

Research output: Contribution to journalJournal articlepeer-review

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Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex. / Zhang, Gang; Kruse, Thomas; Guasch Boldú, Claudia; Garvanska, Dimitriya H; Coscia, Fabian; Mann, Matthias; Barisic, Marin; Nilsson, Jakob.

In: E M B O Journal, Vol. 38, No. 7, e100977, 2019.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Zhang, G, Kruse, T, Guasch Boldú, C, Garvanska, DH, Coscia, F, Mann, M, Barisic, M & Nilsson, J 2019, 'Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex', E M B O Journal, vol. 38, no. 7, e100977. https://doi.org/10.15252/embj.2018100977

APA

Zhang, G., Kruse, T., Guasch Boldú, C., Garvanska, D. H., Coscia, F., Mann, M., Barisic, M., & Nilsson, J. (2019). Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex. E M B O Journal, 38(7), [e100977]. https://doi.org/10.15252/embj.2018100977

Vancouver

Zhang G, Kruse T, Guasch Boldú C, Garvanska DH, Coscia F, Mann M et al. Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex. E M B O Journal. 2019;38(7). e100977. https://doi.org/10.15252/embj.2018100977

Author

Zhang, Gang ; Kruse, Thomas ; Guasch Boldú, Claudia ; Garvanska, Dimitriya H ; Coscia, Fabian ; Mann, Matthias ; Barisic, Marin ; Nilsson, Jakob. / Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex. In: E M B O Journal. 2019 ; Vol. 38, No. 7.

Bibtex

@article{0764a5a5758e4c6cb90e2949a1bddeae,
title = "Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex",
abstract = "Kinetochore localized Mad1 is essential for generating a {"}wait anaphase{"} signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist. By combining genome editing and RNAi, we achieve penetrant removal of Bub1 and Rod in human cells, which reveals that efficient checkpoint signaling depends on the integrated activities of these proteins. Rod removal reduces the proximity of Bub1 and Mad1, and we can bypass the requirement for Rod by tethering Mad1 to kinetochores or increasing the strength of the Bub1-Mad1 interaction. We find that Bub1 has checkpoint functions independent of Mad1 localization that are supported by low levels of Bub1 suggesting a catalytic function. In conclusion, our results support an integrated model for the Mad1 receptors in which the primary role of RZZ is to localize Mad1 at kinetochores to generate the Mad1-Bub1 complex.",
author = "Gang Zhang and Thomas Kruse and {Guasch Bold{\'u}}, Claudia and Garvanska, {Dimitriya H} and Fabian Coscia and Matthias Mann and Marin Barisic and Jakob Nilsson",
note = "{\textcopyright} 2019 The Authors.",
year = "2019",
doi = "10.15252/embj.2018100977",
language = "English",
volume = "38",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "7",

}

RIS

TY - JOUR

T1 - Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex

AU - Zhang, Gang

AU - Kruse, Thomas

AU - Guasch Boldú, Claudia

AU - Garvanska, Dimitriya H

AU - Coscia, Fabian

AU - Mann, Matthias

AU - Barisic, Marin

AU - Nilsson, Jakob

N1 - © 2019 The Authors.

PY - 2019

Y1 - 2019

N2 - Kinetochore localized Mad1 is essential for generating a "wait anaphase" signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist. By combining genome editing and RNAi, we achieve penetrant removal of Bub1 and Rod in human cells, which reveals that efficient checkpoint signaling depends on the integrated activities of these proteins. Rod removal reduces the proximity of Bub1 and Mad1, and we can bypass the requirement for Rod by tethering Mad1 to kinetochores or increasing the strength of the Bub1-Mad1 interaction. We find that Bub1 has checkpoint functions independent of Mad1 localization that are supported by low levels of Bub1 suggesting a catalytic function. In conclusion, our results support an integrated model for the Mad1 receptors in which the primary role of RZZ is to localize Mad1 at kinetochores to generate the Mad1-Bub1 complex.

AB - Kinetochore localized Mad1 is essential for generating a "wait anaphase" signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist. By combining genome editing and RNAi, we achieve penetrant removal of Bub1 and Rod in human cells, which reveals that efficient checkpoint signaling depends on the integrated activities of these proteins. Rod removal reduces the proximity of Bub1 and Mad1, and we can bypass the requirement for Rod by tethering Mad1 to kinetochores or increasing the strength of the Bub1-Mad1 interaction. We find that Bub1 has checkpoint functions independent of Mad1 localization that are supported by low levels of Bub1 suggesting a catalytic function. In conclusion, our results support an integrated model for the Mad1 receptors in which the primary role of RZZ is to localize Mad1 at kinetochores to generate the Mad1-Bub1 complex.

U2 - 10.15252/embj.2018100977

DO - 10.15252/embj.2018100977

M3 - Journal article

C2 - 30782962

VL - 38

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 7

M1 - e100977

ER -

ID: 214021997