Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors. / Ameri, Jacqueline; Borup, Rehannah ; Prawiro, Christy; Ramond, Cyrille; Schachter, Karen; Scharfmann, Raphael ; Semb, Tor Henrik.

In: Cell Reports, Vol. 19, No. 1, 04.04.2017, p. 36-49.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ameri, J, Borup, R, Prawiro, C, Ramond, C, Schachter, K, Scharfmann, R & Semb, TH 2017, 'Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors', Cell Reports, vol. 19, no. 1, pp. 36-49. https://doi.org/10.1016/j.celrep.2017.03.032

APA

Ameri, J., Borup, R., Prawiro, C., Ramond, C., Schachter, K., Scharfmann, R., & Semb, T. H. (2017). Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors. Cell Reports, 19(1), 36-49. https://doi.org/10.1016/j.celrep.2017.03.032

Vancouver

Ameri J, Borup R, Prawiro C, Ramond C, Schachter K, Scharfmann R et al. Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors. Cell Reports. 2017 Apr 4;19(1):36-49. https://doi.org/10.1016/j.celrep.2017.03.032

Author

Ameri, Jacqueline ; Borup, Rehannah ; Prawiro, Christy ; Ramond, Cyrille ; Schachter, Karen ; Scharfmann, Raphael ; Semb, Tor Henrik. / Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors. In: Cell Reports. 2017 ; Vol. 19, No. 1. pp. 36-49.

Bibtex

@article{5a9186e5f9da45d29b2b22b92b602948,
title = "Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors",
abstract = "Stem cell-based therapy for type 1 diabetes would benefit from implementation of a cell purification step at the pancreatic endoderm stage. This would increase the safety of the final cell product, allow the establishment of an intermediate-stage stem cell bank, and provide a means for upscaling β cell manufacturing. Comparative gene expression analysis revealed glycoprotein 2 (GP2) as a specific cell surface marker for isolating pancreatic endoderm cells (PECs) from differentiated hESCs and human fetal pancreas. Isolated GP2+ PECs efficiently differentiated into glucose responsive insulin-producing cells in vitro. We found that in vitro PEC proliferation declines due to enhanced expression of the cyclin-dependent kinase (CDK) inhibitors CDKN1A and CDKN2A. However, we identified a time window when reducing CDKN1A or CDKN2A expression increased proliferation and yield of GP2+ PECs. Altogether, our results contribute tools and concepts toward the isolation and use of PECs as a source for the safe production of hPSC-derived β cells.",
author = "Jacqueline Ameri and Rehannah Borup and Christy Prawiro and Cyrille Ramond and Karen Schachter and Raphael Scharfmann and Semb, {Tor Henrik}",
year = "2017",
month = apr,
day = "4",
doi = "10.1016/j.celrep.2017.03.032",
language = "English",
volume = "19",
pages = "36--49",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors

AU - Ameri, Jacqueline

AU - Borup, Rehannah

AU - Prawiro, Christy

AU - Ramond, Cyrille

AU - Schachter, Karen

AU - Scharfmann, Raphael

AU - Semb, Tor Henrik

PY - 2017/4/4

Y1 - 2017/4/4

N2 - Stem cell-based therapy for type 1 diabetes would benefit from implementation of a cell purification step at the pancreatic endoderm stage. This would increase the safety of the final cell product, allow the establishment of an intermediate-stage stem cell bank, and provide a means for upscaling β cell manufacturing. Comparative gene expression analysis revealed glycoprotein 2 (GP2) as a specific cell surface marker for isolating pancreatic endoderm cells (PECs) from differentiated hESCs and human fetal pancreas. Isolated GP2+ PECs efficiently differentiated into glucose responsive insulin-producing cells in vitro. We found that in vitro PEC proliferation declines due to enhanced expression of the cyclin-dependent kinase (CDK) inhibitors CDKN1A and CDKN2A. However, we identified a time window when reducing CDKN1A or CDKN2A expression increased proliferation and yield of GP2+ PECs. Altogether, our results contribute tools and concepts toward the isolation and use of PECs as a source for the safe production of hPSC-derived β cells.

AB - Stem cell-based therapy for type 1 diabetes would benefit from implementation of a cell purification step at the pancreatic endoderm stage. This would increase the safety of the final cell product, allow the establishment of an intermediate-stage stem cell bank, and provide a means for upscaling β cell manufacturing. Comparative gene expression analysis revealed glycoprotein 2 (GP2) as a specific cell surface marker for isolating pancreatic endoderm cells (PECs) from differentiated hESCs and human fetal pancreas. Isolated GP2+ PECs efficiently differentiated into glucose responsive insulin-producing cells in vitro. We found that in vitro PEC proliferation declines due to enhanced expression of the cyclin-dependent kinase (CDK) inhibitors CDKN1A and CDKN2A. However, we identified a time window when reducing CDKN1A or CDKN2A expression increased proliferation and yield of GP2+ PECs. Altogether, our results contribute tools and concepts toward the isolation and use of PECs as a source for the safe production of hPSC-derived β cells.

U2 - 10.1016/j.celrep.2017.03.032

DO - 10.1016/j.celrep.2017.03.032

M3 - Journal article

C2 - 28380361

VL - 19

SP - 36

EP - 49

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 1

ER -

ID: 174896633