Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction

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Standard

Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction. / Peña-Espinoza, Miguel; Thamsborg, Stig Milan; Denwood, Matt; Drag, Markus; Hansen, Tina Vicky Alstrup; Jensen, Vibeke F.; Enemark, Heidi L.

In: International Journal for Parasitology: Drugs and Drug Resistance, Vol. 6, No. 3, 12.2016, p. 241-250.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Peña-Espinoza, M, Thamsborg, SM, Denwood, M, Drag, M, Hansen, TVA, Jensen, VF & Enemark, HL 2016, 'Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction', International Journal for Parasitology: Drugs and Drug Resistance, vol. 6, no. 3, pp. 241-250. https://doi.org/10.1016/j.ijpddr.2016.10.004

APA

Peña-Espinoza, M., Thamsborg, S. M., Denwood, M., Drag, M., Hansen, T. V. A., Jensen, V. F., & Enemark, H. L. (2016). Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction. International Journal for Parasitology: Drugs and Drug Resistance, 6(3), 241-250. https://doi.org/10.1016/j.ijpddr.2016.10.004

Vancouver

Peña-Espinoza M, Thamsborg SM, Denwood M, Drag M, Hansen TVA, Jensen VF et al. Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction. International Journal for Parasitology: Drugs and Drug Resistance. 2016 Dec;6(3):241-250. https://doi.org/10.1016/j.ijpddr.2016.10.004

Author

Peña-Espinoza, Miguel ; Thamsborg, Stig Milan ; Denwood, Matt ; Drag, Markus ; Hansen, Tina Vicky Alstrup ; Jensen, Vibeke F. ; Enemark, Heidi L. / Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction. In: International Journal for Parasitology: Drugs and Drug Resistance. 2016 ; Vol. 6, No. 3. pp. 241-250.

Bibtex

@article{57697955652245ad8aa6bc4f60b3f59b,

title = "Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction",

abstract = "The efficacy of ivermectin (IVM) against gastrointestinal nematodes in Danish cattle was assessed by faecal egg count reduction test (FECRT). Six cattle farms with history of clinical parasitism and avermectin use were included. On the day of treatment (Day 0), 20 naturally infected calves per farm (total n = 120) were stratified by initial faecal egg counts (FEC) and randomly allocated to a treatment group dosed with 0.2 mg IVM kg(-1) body weight s.c. (IVM; n = 10) or an untreated control group (CTL; n = 10). Individual FEC were obtained at Day 0 and Day 14 post-treatment and pooled faeces by group were cultured to isolate L3 for detection of Ostertagia ostertagi and Cooperia oncophora by qPCR. Treatment efficacies were analysed using the recommended WAAVP method and two open-source statistical procedures based on Bayesian modelling: 'eggCounts' and 'Bayescount'. A simulation study evaluated the performance of the different procedures to correctly identify FEC reduction percentages of simulated bovine FEC data representing the observed real data. In the FECRT, reduced IVM efficacy was detected in three farms by all procedures using data from treated animals only, and in one farm according to the procedures including data from treated and untreated cattle. Post-treatment, O. ostertagi and C. oncophora L3 were detected by qPCR in faeces of treated animals from one and three herds with declared reduced IVM efficacy, respectively. Based on the simulation study, all methods showed a reduced performance when FEC aggregation increased post-treatment and suggested that a treatment group of 10 animals is insufficient for the FECRT in cattle. This is the first report of reduced anthelmintic efficacy in Danish cattle and warrants the implementation of larger surveys. Advantages and caveats regarding the use of Bayesian modelling and the relevance of including untreated cattle in the FECRT are discussed.",

author = "Miguel Pe{\~n}a-Espinoza and Thamsborg, {Stig Milan} and Matt Denwood and Markus Drag and Hansen, {Tina Vicky Alstrup} and Jensen, {Vibeke F.} and Enemark, {Heidi L.}",

year = "2016",

month = dec,

doi = "10.1016/j.ijpddr.2016.10.004",

language = "English",

volume = "6",

pages = "241--250",

journal = "International Journal for Parasitology",

issn = "0020-7519",

publisher = "Elsevier",

number = "3",

}

RIS

TY - JOUR

T1 - Efficacy of ivermectin against gastrointestinal nematodes of cattle in Denmark evaluated by different methods for analysis of faecal egg count reduction

AU - Peña-Espinoza, Miguel

AU - Thamsborg, Stig Milan

AU - Denwood, Matt

AU - Drag, Markus

AU - Hansen, Tina Vicky Alstrup

AU - Jensen, Vibeke F.

AU - Enemark, Heidi L.

PY - 2016/12

Y1 - 2016/12

N2 - The efficacy of ivermectin (IVM) against gastrointestinal nematodes in Danish cattle was assessed by faecal egg count reduction test (FECRT). Six cattle farms with history of clinical parasitism and avermectin use were included. On the day of treatment (Day 0), 20 naturally infected calves per farm (total n = 120) were stratified by initial faecal egg counts (FEC) and randomly allocated to a treatment group dosed with 0.2 mg IVM kg(-1) body weight s.c. (IVM; n = 10) or an untreated control group (CTL; n = 10). Individual FEC were obtained at Day 0 and Day 14 post-treatment and pooled faeces by group were cultured to isolate L3 for detection of Ostertagia ostertagi and Cooperia oncophora by qPCR. Treatment efficacies were analysed using the recommended WAAVP method and two open-source statistical procedures based on Bayesian modelling: 'eggCounts' and 'Bayescount'. A simulation study evaluated the performance of the different procedures to correctly identify FEC reduction percentages of simulated bovine FEC data representing the observed real data. In the FECRT, reduced IVM efficacy was detected in three farms by all procedures using data from treated animals only, and in one farm according to the procedures including data from treated and untreated cattle. Post-treatment, O. ostertagi and C. oncophora L3 were detected by qPCR in faeces of treated animals from one and three herds with declared reduced IVM efficacy, respectively. Based on the simulation study, all methods showed a reduced performance when FEC aggregation increased post-treatment and suggested that a treatment group of 10 animals is insufficient for the FECRT in cattle. This is the first report of reduced anthelmintic efficacy in Danish cattle and warrants the implementation of larger surveys. Advantages and caveats regarding the use of Bayesian modelling and the relevance of including untreated cattle in the FECRT are discussed.

AB - The efficacy of ivermectin (IVM) against gastrointestinal nematodes in Danish cattle was assessed by faecal egg count reduction test (FECRT). Six cattle farms with history of clinical parasitism and avermectin use were included. On the day of treatment (Day 0), 20 naturally infected calves per farm (total n = 120) were stratified by initial faecal egg counts (FEC) and randomly allocated to a treatment group dosed with 0.2 mg IVM kg(-1) body weight s.c. (IVM; n = 10) or an untreated control group (CTL; n = 10). Individual FEC were obtained at Day 0 and Day 14 post-treatment and pooled faeces by group were cultured to isolate L3 for detection of Ostertagia ostertagi and Cooperia oncophora by qPCR. Treatment efficacies were analysed using the recommended WAAVP method and two open-source statistical procedures based on Bayesian modelling: 'eggCounts' and 'Bayescount'. A simulation study evaluated the performance of the different procedures to correctly identify FEC reduction percentages of simulated bovine FEC data representing the observed real data. In the FECRT, reduced IVM efficacy was detected in three farms by all procedures using data from treated animals only, and in one farm according to the procedures including data from treated and untreated cattle. Post-treatment, O. ostertagi and C. oncophora L3 were detected by qPCR in faeces of treated animals from one and three herds with declared reduced IVM efficacy, respectively. Based on the simulation study, all methods showed a reduced performance when FEC aggregation increased post-treatment and suggested that a treatment group of 10 animals is insufficient for the FECRT in cattle. This is the first report of reduced anthelmintic efficacy in Danish cattle and warrants the implementation of larger surveys. Advantages and caveats regarding the use of Bayesian modelling and the relevance of including untreated cattle in the FECRT are discussed.

U2 - 10.1016/j.ijpddr.2016.10.004

DO - 10.1016/j.ijpddr.2016.10.004

M3 - Journal article

C2 - 27835769

VL - 6

SP - 241

EP - 250

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 3

ER -

ID: 168904802