Effective representation and storage of mass spectrometry-based proteomic data sets for the scientific community
Research output: Contribution to journal › Journal article › Research › peer-review
Mass spectrometry-based proteomics has emerged as a technology of choice for global analysis of cell signaling networks. However, reporting and sharing of MS data are often haphazard, limiting the usefulness of proteomics to the signaling community. We argue that raw data should always be provided with proteomics studies together with detailed peptide and protein identification and quantification information. Statistical criteria for peptide identification and their posttranslational modifications have largely been established for individual projects. However, the current practice of indiscriminately incorporating these individual results into databases such as UniProt is problematic. Because of the vast differences in underlying data quality, we advocate a differentiated annotation of data by level of reliability. Requirements for the reporting of quantitative data are being developed, but there are few mechanisms for community-wide sharing of these data.
Original language | English |
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Journal | Science Signaling |
Volume | 4 |
Issue number | 160 |
Pages (from-to) | pe7 |
ISSN | 1945-0877 |
DOIs | |
Publication status | Published - 1 Jan 2011 |
- Database Management Systems, Databases, Protein, Humans, Mass Spectrometry, Proteomics, Signal Transduction
Research areas
ID: 34339699