Effect of hypoxic exercise on glucose tolerance in healthy and pre-diabetic adults

Research output: Contribution to journalJournal articlepeer-review

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Effect of hypoxic exercise on glucose tolerance in healthy and pre-diabetic adults. / De Groote, Estelle; Britto, Florian A; Balan, Estelle; Warnier, Geoffrey; Thissen, Jean-Paul; Nielens, Henri; Sylow, Lykke; Deldicque, Louise.

In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 320, No. 1, 2021, p. E43-E54.

Research output: Contribution to journalJournal articlepeer-review

Harvard

De Groote, E, Britto, FA, Balan, E, Warnier, G, Thissen, J-P, Nielens, H, Sylow, L & Deldicque, L 2021, 'Effect of hypoxic exercise on glucose tolerance in healthy and pre-diabetic adults', American Journal of Physiology: Endocrinology and Metabolism, vol. 320, no. 1, pp. E43-E54. https://doi.org/10.1152/ajpendo.00263.2020

APA

De Groote, E., Britto, F. A., Balan, E., Warnier, G., Thissen, J-P., Nielens, H., Sylow, L., & Deldicque, L. (2021). Effect of hypoxic exercise on glucose tolerance in healthy and pre-diabetic adults. American Journal of Physiology: Endocrinology and Metabolism, 320(1), E43-E54. https://doi.org/10.1152/ajpendo.00263.2020

Vancouver

De Groote E, Britto FA, Balan E, Warnier G, Thissen J-P, Nielens H et al. Effect of hypoxic exercise on glucose tolerance in healthy and pre-diabetic adults. American Journal of Physiology: Endocrinology and Metabolism. 2021;320(1):E43-E54. https://doi.org/10.1152/ajpendo.00263.2020

Author

De Groote, Estelle ; Britto, Florian A ; Balan, Estelle ; Warnier, Geoffrey ; Thissen, Jean-Paul ; Nielens, Henri ; Sylow, Lykke ; Deldicque, Louise. / Effect of hypoxic exercise on glucose tolerance in healthy and pre-diabetic adults. In: American Journal of Physiology: Endocrinology and Metabolism. 2021 ; Vol. 320, No. 1. pp. E43-E54.

Bibtex

@article{18b76aa3b9cb484180b2a3f9f2d980f3,
title = "Effect of hypoxic exercise on glucose tolerance in healthy and pre-diabetic adults",
abstract = "Purpose: This study aimed to investigate the mechanisms known to regulate glucose homeostasis in human skeletal muscle of healthy and pre-diabetic subjects exercising in normobaric hypoxia.Methods: Seventeen healthy (H; 28.8±2.4y; VO2max: 45.1±1.8mL·kg-1·min-1) and fifteen pre-diabetic (P; 44.6±3.9y; VO2max: 30.8±2.5mL·kg-1·min-1) men were randomly assigned to two groups performing an acute exercise bout (heart rate corresponding to 55% VO2max) either in normoxic (NE) or in hypoxic (HE; FiO2 14.0%) conditions. An oral glucose tolerance test (OGTT) was performed in a basal state and after an acute exercise bout. Muscle biopsies from m. vastus lateralis were taken before and after exercise. Venous blood samples were taken at regular intervals before, during and after exercise.Results: The two groups exercising in hypoxia had a larger area under the curve of blood glucose levels during the OGTT after exercise compared to baseline (H: +4%; P: +11%). Compared to pre-exercise, an increase in p-TBC1D1 Ser237 and in p-AMPK Thr172 was observed post-exercise in P NE (+95%; +55%, respectively) and H HE (+91%; +43%, respectively). An increase in p-ACC Ser212 was measured after exercise in all groups (H NE: +228%; P NE: +252%; H HE: +252%; P HE: +208%).Conclusions: Our results show that an acute bout of exercise in hypoxia reduces glucose tolerance in healthy and pre-diabetic subjects. At a molecular level, some adaptations regulating glucose transport in muscle were found in all groups without associations with glucose tolerance after exercise. The results suggest that hypoxia negatively affects glucose tolerance post-exercise through unidentified mechanisms.",
keywords = "Faculty of Science, Diabetes, Cycling, OGTT, GLUT4, AMPK",
author = "{De Groote}, Estelle and Britto, {Florian A} and Estelle Balan and Geoffrey Warnier and Jean-Paul Thissen and Henri Nielens and Lykke Sylow and Louise Deldicque",
note = "CURIS 2021 NEXS 015",
year = "2021",
doi = "10.1152/ajpendo.00263.2020",
language = "English",
volume = "320",
pages = "E43--E54",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "1",

}

RIS

TY - JOUR

T1 - Effect of hypoxic exercise on glucose tolerance in healthy and pre-diabetic adults

AU - De Groote, Estelle

AU - Britto, Florian A

AU - Balan, Estelle

AU - Warnier, Geoffrey

AU - Thissen, Jean-Paul

AU - Nielens, Henri

AU - Sylow, Lykke

AU - Deldicque, Louise

N1 - CURIS 2021 NEXS 015

PY - 2021

Y1 - 2021

N2 - Purpose: This study aimed to investigate the mechanisms known to regulate glucose homeostasis in human skeletal muscle of healthy and pre-diabetic subjects exercising in normobaric hypoxia.Methods: Seventeen healthy (H; 28.8±2.4y; VO2max: 45.1±1.8mL·kg-1·min-1) and fifteen pre-diabetic (P; 44.6±3.9y; VO2max: 30.8±2.5mL·kg-1·min-1) men were randomly assigned to two groups performing an acute exercise bout (heart rate corresponding to 55% VO2max) either in normoxic (NE) or in hypoxic (HE; FiO2 14.0%) conditions. An oral glucose tolerance test (OGTT) was performed in a basal state and after an acute exercise bout. Muscle biopsies from m. vastus lateralis were taken before and after exercise. Venous blood samples were taken at regular intervals before, during and after exercise.Results: The two groups exercising in hypoxia had a larger area under the curve of blood glucose levels during the OGTT after exercise compared to baseline (H: +4%; P: +11%). Compared to pre-exercise, an increase in p-TBC1D1 Ser237 and in p-AMPK Thr172 was observed post-exercise in P NE (+95%; +55%, respectively) and H HE (+91%; +43%, respectively). An increase in p-ACC Ser212 was measured after exercise in all groups (H NE: +228%; P NE: +252%; H HE: +252%; P HE: +208%).Conclusions: Our results show that an acute bout of exercise in hypoxia reduces glucose tolerance in healthy and pre-diabetic subjects. At a molecular level, some adaptations regulating glucose transport in muscle were found in all groups without associations with glucose tolerance after exercise. The results suggest that hypoxia negatively affects glucose tolerance post-exercise through unidentified mechanisms.

AB - Purpose: This study aimed to investigate the mechanisms known to regulate glucose homeostasis in human skeletal muscle of healthy and pre-diabetic subjects exercising in normobaric hypoxia.Methods: Seventeen healthy (H; 28.8±2.4y; VO2max: 45.1±1.8mL·kg-1·min-1) and fifteen pre-diabetic (P; 44.6±3.9y; VO2max: 30.8±2.5mL·kg-1·min-1) men were randomly assigned to two groups performing an acute exercise bout (heart rate corresponding to 55% VO2max) either in normoxic (NE) or in hypoxic (HE; FiO2 14.0%) conditions. An oral glucose tolerance test (OGTT) was performed in a basal state and after an acute exercise bout. Muscle biopsies from m. vastus lateralis were taken before and after exercise. Venous blood samples were taken at regular intervals before, during and after exercise.Results: The two groups exercising in hypoxia had a larger area under the curve of blood glucose levels during the OGTT after exercise compared to baseline (H: +4%; P: +11%). Compared to pre-exercise, an increase in p-TBC1D1 Ser237 and in p-AMPK Thr172 was observed post-exercise in P NE (+95%; +55%, respectively) and H HE (+91%; +43%, respectively). An increase in p-ACC Ser212 was measured after exercise in all groups (H NE: +228%; P NE: +252%; H HE: +252%; P HE: +208%).Conclusions: Our results show that an acute bout of exercise in hypoxia reduces glucose tolerance in healthy and pre-diabetic subjects. At a molecular level, some adaptations regulating glucose transport in muscle were found in all groups without associations with glucose tolerance after exercise. The results suggest that hypoxia negatively affects glucose tolerance post-exercise through unidentified mechanisms.

KW - Faculty of Science

KW - Diabetes

KW - Cycling

KW - OGTT

KW - GLUT4

KW - AMPK

U2 - 10.1152/ajpendo.00263.2020

DO - 10.1152/ajpendo.00263.2020

M3 - Journal article

C2 - 33103453

VL - 320

SP - E43-E54

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 1

ER -

ID: 250483268