Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing. / Karlsen, Anders; Yeung, Ching-Yan Chloé; Schjerling, Peter; Denz, Linda; Hoegsbjerg, Christian; Jakobsen, Jens Rithamer; Krogsgaard, Michael; Koch, Manuel; Schiaffino, Stefano; Kjaer, Michael; Mackey, Abigail.

In: Journal of Cell Science, Vol. 136, No. 8, jcs260913, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Karlsen, A, Yeung, C-YC, Schjerling, P, Denz, L, Hoegsbjerg, C, Jakobsen, JR, Krogsgaard, M, Koch, M, Schiaffino, S, Kjaer, M & Mackey, A 2023, 'Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing', Journal of Cell Science, vol. 136, no. 8, jcs260913. https://doi.org/10.1242/jcs.260913

APA

Karlsen, A., Yeung, C-Y. C., Schjerling, P., Denz, L., Hoegsbjerg, C., Jakobsen, J. R., Krogsgaard, M., Koch, M., Schiaffino, S., Kjaer, M., & Mackey, A. (2023). Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing. Journal of Cell Science, 136(8), [jcs260913]. https://doi.org/10.1242/jcs.260913

Vancouver

Karlsen A, Yeung C-YC, Schjerling P, Denz L, Hoegsbjerg C, Jakobsen JR et al. Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing. Journal of Cell Science. 2023;136(8). jcs260913. https://doi.org/10.1242/jcs.260913

Author

Karlsen, Anders ; Yeung, Ching-Yan Chloé ; Schjerling, Peter ; Denz, Linda ; Hoegsbjerg, Christian ; Jakobsen, Jens Rithamer ; Krogsgaard, Michael ; Koch, Manuel ; Schiaffino, Stefano ; Kjaer, Michael ; Mackey, Abigail. / Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing. In: Journal of Cell Science. 2023 ; Vol. 136, No. 8.

Bibtex

@article{c1f43d574d084b679f476f3b5f595d39,
title = "Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing",
abstract = "The myotendinous junction (MTJ) is a specialized domain of the multinucleated myofibre, faced with the challenge of maintaining robust cell-matrix contact with the tendon under high mechanical stress and strain. Here, we profiled 24,161 nuclei in semitendinosus muscle-tendon samples from 3 healthy males by single nucleus RNA-sequencing (snRNA-seq), alongside spatial transcriptomics, to gain insight into the genes characterizing this specialization in humans. We identified a cluster of MTJ myonuclei, represented by 47 enriched transcripts, of which the presence of ABI3BP, ABLIM1, ADAMTSL1, BICD1, CPM, FHOD3, FRAS1 and FREM2 was confirmed at the MTJ at the protein level by immunofluorescence. Four distinct subclusters of MTJ myonuclei were apparent and segregated into two COL22A1-expressing subclusters and two lacking COL22A1 but with a clear fibre type profile expressing MYH7 or MYH1/2. Our findings reveal distinct myonuclei profiles of the human MTJ, a weak link in the musculoskeletal system, which is selectively affected in pathological conditions, from muscle strains to muscular dystrophies.",
author = "Anders Karlsen and Yeung, {Ching-Yan Chlo{\'e}} and Peter Schjerling and Linda Denz and Christian Hoegsbjerg and Jakobsen, {Jens Rithamer} and Michael Krogsgaard and Manuel Koch and Stefano Schiaffino and Michael Kjaer and Abigail Mackey",
year = "2023",
doi = "10.1242/jcs.260913",
language = "English",
volume = "136",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "The/Company of Biologists Ltd.",
number = "8",

}

RIS

TY - JOUR

T1 - Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing

AU - Karlsen, Anders

AU - Yeung, Ching-Yan Chloé

AU - Schjerling, Peter

AU - Denz, Linda

AU - Hoegsbjerg, Christian

AU - Jakobsen, Jens Rithamer

AU - Krogsgaard, Michael

AU - Koch, Manuel

AU - Schiaffino, Stefano

AU - Kjaer, Michael

AU - Mackey, Abigail

PY - 2023

Y1 - 2023

N2 - The myotendinous junction (MTJ) is a specialized domain of the multinucleated myofibre, faced with the challenge of maintaining robust cell-matrix contact with the tendon under high mechanical stress and strain. Here, we profiled 24,161 nuclei in semitendinosus muscle-tendon samples from 3 healthy males by single nucleus RNA-sequencing (snRNA-seq), alongside spatial transcriptomics, to gain insight into the genes characterizing this specialization in humans. We identified a cluster of MTJ myonuclei, represented by 47 enriched transcripts, of which the presence of ABI3BP, ABLIM1, ADAMTSL1, BICD1, CPM, FHOD3, FRAS1 and FREM2 was confirmed at the MTJ at the protein level by immunofluorescence. Four distinct subclusters of MTJ myonuclei were apparent and segregated into two COL22A1-expressing subclusters and two lacking COL22A1 but with a clear fibre type profile expressing MYH7 or MYH1/2. Our findings reveal distinct myonuclei profiles of the human MTJ, a weak link in the musculoskeletal system, which is selectively affected in pathological conditions, from muscle strains to muscular dystrophies.

AB - The myotendinous junction (MTJ) is a specialized domain of the multinucleated myofibre, faced with the challenge of maintaining robust cell-matrix contact with the tendon under high mechanical stress and strain. Here, we profiled 24,161 nuclei in semitendinosus muscle-tendon samples from 3 healthy males by single nucleus RNA-sequencing (snRNA-seq), alongside spatial transcriptomics, to gain insight into the genes characterizing this specialization in humans. We identified a cluster of MTJ myonuclei, represented by 47 enriched transcripts, of which the presence of ABI3BP, ABLIM1, ADAMTSL1, BICD1, CPM, FHOD3, FRAS1 and FREM2 was confirmed at the MTJ at the protein level by immunofluorescence. Four distinct subclusters of MTJ myonuclei were apparent and segregated into two COL22A1-expressing subclusters and two lacking COL22A1 but with a clear fibre type profile expressing MYH7 or MYH1/2. Our findings reveal distinct myonuclei profiles of the human MTJ, a weak link in the musculoskeletal system, which is selectively affected in pathological conditions, from muscle strains to muscular dystrophies.

U2 - 10.1242/jcs.260913

DO - 10.1242/jcs.260913

M3 - Journal article

C2 - 36924352

VL - 136

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 8

M1 - jcs260913

ER -

ID: 370203354