Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark

Research output: Contribution to journalJournal articlepeer-review

Standard

Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark. / Samuelsen, Ørjan; Hansen, Frank; Aasnæs, Bettina; Hasman, Henrik; Lund, Bjarte Aarmo; Leiros, Hanna Kirsti S.; Lilje, Berit; Janice, Jessin; Jakobsen, Lotte; Littauer, Pia; Søes, Lillian M.; Holzknecht, Barbara J.; Andersen, Leif P.; Stegger, Marc; Andersen, Paal S.; Hammerum, Anette M.

In: Antimicrobial Agents and Chemotherapy, Vol. 62, No. 1, e01260-17, 01.2018.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Samuelsen, Ø, Hansen, F, Aasnæs, B, Hasman, H, Lund, BA, Leiros, HKS, Lilje, B, Janice, J, Jakobsen, L, Littauer, P, Søes, LM, Holzknecht, BJ, Andersen, LP, Stegger, M, Andersen, PS & Hammerum, AM 2018, 'Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark', Antimicrobial Agents and Chemotherapy, vol. 62, no. 1, e01260-17. https://doi.org/10.1128/AAC.01260-17

APA

Samuelsen, Ø., Hansen, F., Aasnæs, B., Hasman, H., Lund, B. A., Leiros, H. K. S., Lilje, B., Janice, J., Jakobsen, L., Littauer, P., Søes, L. M., Holzknecht, B. J., Andersen, L. P., Stegger, M., Andersen, P. S., & Hammerum, A. M. (2018). Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark. Antimicrobial Agents and Chemotherapy, 62(1), [e01260-17]. https://doi.org/10.1128/AAC.01260-17

Vancouver

Samuelsen Ø, Hansen F, Aasnæs B, Hasman H, Lund BA, Leiros HKS et al. Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark. Antimicrobial Agents and Chemotherapy. 2018 Jan;62(1). e01260-17. https://doi.org/10.1128/AAC.01260-17

Author

Samuelsen, Ørjan ; Hansen, Frank ; Aasnæs, Bettina ; Hasman, Henrik ; Lund, Bjarte Aarmo ; Leiros, Hanna Kirsti S. ; Lilje, Berit ; Janice, Jessin ; Jakobsen, Lotte ; Littauer, Pia ; Søes, Lillian M. ; Holzknecht, Barbara J. ; Andersen, Leif P. ; Stegger, Marc ; Andersen, Paal S. ; Hammerum, Anette M. / Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark. In: Antimicrobial Agents and Chemotherapy. 2018 ; Vol. 62, No. 1.

Bibtex

@article{fd65fb9e39534676b7e6f3d13a69de21,
title = "Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark",
abstract = "The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D β-lactamase (CHDL) named OXA-436. In total, six OXA-436-producing Enterobacteriaceae isolates, including Enterobacter asburiae (n = 3), Citrobacter freundii (n = 2), and Klebsiella pneumoniae (n = 1), were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing Enterobacteriaceae were found in one patient. The amino acid sequence of OXA-436 showed 90.4 to 92.8% identity to the amino acid sequences of other acquired OXA-48-like variants. Expression of OXA-436 in Escherichia coli and kinetic analysis of purified OXA-436 revealed an activity profile similar to that of OXA-48 and OXA-181, with activity against penicillins, including temocillin; limited or no activity against extended-spectrum cephalosporins; and activity against carbapenems. The blaOXA-436 gene was located on a conjugative ∼314-kb IncHI2/IncHI2A plasmid belonging to plasmid multilocus sequence typing sequence type 1 in a region surrounded by chromosomal genes previously identified to be adjacent to blaOXA genes in Shewanella spp. In conclusion, OXA-436 is a novel CHDL with functional properties similar to those of OXA-48-like CHDLs. The described geographical spread among different Enterobacteriaceae and the plasmid location of blaOXA-436 illustrate its potential for further dissemination.",
keywords = "Carbapenems",
author = "{\O}rjan Samuelsen and Frank Hansen and Bettina Aasn{\ae}s and Henrik Hasman and Lund, {Bjarte Aarmo} and Leiros, {Hanna Kirsti S.} and Berit Lilje and Jessin Janice and Lotte Jakobsen and Pia Littauer and S{\o}es, {Lillian M.} and Holzknecht, {Barbara J.} and Andersen, {Leif P.} and Marc Stegger and Andersen, {Paal S.} and Hammerum, {Anette M.}",
year = "2018",
month = jan,
doi = "10.1128/AAC.01260-17",
language = "English",
volume = "62",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "1",

}

RIS

TY - JOUR

T1 - Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark

AU - Samuelsen, Ørjan

AU - Hansen, Frank

AU - Aasnæs, Bettina

AU - Hasman, Henrik

AU - Lund, Bjarte Aarmo

AU - Leiros, Hanna Kirsti S.

AU - Lilje, Berit

AU - Janice, Jessin

AU - Jakobsen, Lotte

AU - Littauer, Pia

AU - Søes, Lillian M.

AU - Holzknecht, Barbara J.

AU - Andersen, Leif P.

AU - Stegger, Marc

AU - Andersen, Paal S.

AU - Hammerum, Anette M.

PY - 2018/1

Y1 - 2018/1

N2 - The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D β-lactamase (CHDL) named OXA-436. In total, six OXA-436-producing Enterobacteriaceae isolates, including Enterobacter asburiae (n = 3), Citrobacter freundii (n = 2), and Klebsiella pneumoniae (n = 1), were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing Enterobacteriaceae were found in one patient. The amino acid sequence of OXA-436 showed 90.4 to 92.8% identity to the amino acid sequences of other acquired OXA-48-like variants. Expression of OXA-436 in Escherichia coli and kinetic analysis of purified OXA-436 revealed an activity profile similar to that of OXA-48 and OXA-181, with activity against penicillins, including temocillin; limited or no activity against extended-spectrum cephalosporins; and activity against carbapenems. The blaOXA-436 gene was located on a conjugative ∼314-kb IncHI2/IncHI2A plasmid belonging to plasmid multilocus sequence typing sequence type 1 in a region surrounded by chromosomal genes previously identified to be adjacent to blaOXA genes in Shewanella spp. In conclusion, OXA-436 is a novel CHDL with functional properties similar to those of OXA-48-like CHDLs. The described geographical spread among different Enterobacteriaceae and the plasmid location of blaOXA-436 illustrate its potential for further dissemination.

AB - The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D β-lactamase (CHDL) named OXA-436. In total, six OXA-436-producing Enterobacteriaceae isolates, including Enterobacter asburiae (n = 3), Citrobacter freundii (n = 2), and Klebsiella pneumoniae (n = 1), were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing Enterobacteriaceae were found in one patient. The amino acid sequence of OXA-436 showed 90.4 to 92.8% identity to the amino acid sequences of other acquired OXA-48-like variants. Expression of OXA-436 in Escherichia coli and kinetic analysis of purified OXA-436 revealed an activity profile similar to that of OXA-48 and OXA-181, with activity against penicillins, including temocillin; limited or no activity against extended-spectrum cephalosporins; and activity against carbapenems. The blaOXA-436 gene was located on a conjugative ∼314-kb IncHI2/IncHI2A plasmid belonging to plasmid multilocus sequence typing sequence type 1 in a region surrounded by chromosomal genes previously identified to be adjacent to blaOXA genes in Shewanella spp. In conclusion, OXA-436 is a novel CHDL with functional properties similar to those of OXA-48-like CHDLs. The described geographical spread among different Enterobacteriaceae and the plasmid location of blaOXA-436 illustrate its potential for further dissemination.

KW - Carbapenems

U2 - 10.1128/AAC.01260-17

DO - 10.1128/AAC.01260-17

M3 - Journal article

C2 - 29061750

AN - SCOPUS:85039785235

VL - 62

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 1

M1 - e01260-17

ER -

ID: 188752716