Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells. / Jacobsen, Nicklas Raun; Møller, Peter; Cohn, Corey Alexander; Loft, Steffen; Vogel, Ulla; Wallin, Håkan.

In: Mutation Research - Reviews, Vol. 641, No. 1-2, 2008, p. 54-7.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Jacobsen, NR, Møller, P, Cohn, CA, Loft, S, Vogel, U & Wallin, H 2008, 'Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells', Mutation Research - Reviews, vol. 641, no. 1-2, pp. 54-7. https://doi.org/10.1016/j.mrfmmm.2008.03.001

APA

Jacobsen, N. R., Møller, P., Cohn, C. A., Loft, S., Vogel, U., & Wallin, H. (2008). Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells. Mutation Research - Reviews, 641(1-2), 54-7. https://doi.org/10.1016/j.mrfmmm.2008.03.001

Vancouver

Jacobsen NR, Møller P, Cohn CA, Loft S, Vogel U, Wallin H. Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells. Mutation Research - Reviews. 2008;641(1-2):54-7. https://doi.org/10.1016/j.mrfmmm.2008.03.001

Author

Jacobsen, Nicklas Raun ; Møller, Peter ; Cohn, Corey Alexander ; Loft, Steffen ; Vogel, Ulla ; Wallin, Håkan. / Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells. In: Mutation Research - Reviews. 2008 ; Vol. 641, No. 1-2. pp. 54-7.

Bibtex

@article{416e7780e93511ddbf70000ea68e967b,
title = "Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells",
abstract = "The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95{\%} CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95{\%} CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis.",
author = "Jacobsen, {Nicklas Raun} and Peter M{\o}ller and Cohn, {Corey Alexander} and Steffen Loft and Ulla Vogel and H{\aa}kan Wallin",
note = "Keywords: Air Pollutants; Animals; Cells, Cultured; Epithelial Cells; Lung; Mice; Mutagenesis; Mutagens; Reactive Oxygen Species; Soot; Vehicle Emissions",
year = "2008",
doi = "10.1016/j.mrfmmm.2008.03.001",
language = "English",
volume = "641",
pages = "54--7",
journal = "Mutation Research - Reviews",
issn = "1383-5742",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells

AU - Jacobsen, Nicklas Raun

AU - Møller, Peter

AU - Cohn, Corey Alexander

AU - Loft, Steffen

AU - Vogel, Ulla

AU - Wallin, Håkan

N1 - Keywords: Air Pollutants; Animals; Cells, Cultured; Epithelial Cells; Lung; Mice; Mutagenesis; Mutagens; Reactive Oxygen Species; Soot; Vehicle Emissions

PY - 2008

Y1 - 2008

N2 - The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95% CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95% CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis.

AB - The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95% CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95% CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis.

U2 - 10.1016/j.mrfmmm.2008.03.001

DO - 10.1016/j.mrfmmm.2008.03.001

M3 - Journal article

C2 - 18423769

VL - 641

SP - 54

EP - 57

JO - Mutation Research - Reviews

JF - Mutation Research - Reviews

SN - 1383-5742

IS - 1-2

ER -

ID: 9909428