Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit

Research output: Contribution to journalJournal articlepeer-review

Standard

Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit. / Jacobsen, Natashia Mai Yde; Nedergaard, Hanne Bjørn; Kock, Anette; Caglayan, Ibrahim; Laursen, Marie Munch; Lange, Eva-Marie; Marcial-Coba, Martín Sebastián; Bar-Shalom, Daniel; Nielsen, Dennis Sandris; Müllertz, Anette.

In: LWT, Vol. 144, 111174, 2021.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Jacobsen, NMY, Nedergaard, HB, Kock, A, Caglayan, I, Laursen, MM, Lange, E-M, Marcial-Coba, MS, Bar-Shalom, D, Nielsen, DS & Müllertz, A 2021, 'Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit', LWT, vol. 144, 111174. https://doi.org/10.1016/j.lwt.2021.111174

APA

Jacobsen, N. M. Y., Nedergaard, H. B., Kock, A., Caglayan, I., Laursen, M. M., Lange, E-M., Marcial-Coba, M. S., Bar-Shalom, D., Nielsen, D. S., & Müllertz, A. (2021). Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit. LWT, 144, [111174]. https://doi.org/10.1016/j.lwt.2021.111174

Vancouver

Jacobsen NMY, Nedergaard HB, Kock A, Caglayan I, Laursen MM, Lange E-M et al. Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit. LWT. 2021;144. 111174. https://doi.org/10.1016/j.lwt.2021.111174

Author

Jacobsen, Natashia Mai Yde ; Nedergaard, Hanne Bjørn ; Kock, Anette ; Caglayan, Ibrahim ; Laursen, Marie Munch ; Lange, Eva-Marie ; Marcial-Coba, Martín Sebastián ; Bar-Shalom, Daniel ; Nielsen, Dennis Sandris ; Müllertz, Anette. / Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit. In: LWT. 2021 ; Vol. 144.

Bibtex

@article{dabd41e1d29d4ed882437ee67b2205e6,
title = "Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit",
abstract = "Probiotics have become one of the most consumed food supplements worldwide. Many probiotic strains are sensitive to low pH and bile concentrations encountered in the gastrointestinal tract upon oral ingestion. This study aimed at developing gastro-resistant probiotic granulates releasing viable cells in the small intestine. Fatty alcohols were investigated as potential coating materials. Cetostearyl alcohol was selected and combined with different plasticizers to further optimize the coating properties. Combining cetostearyl alcohol with olive oil and beeswax in selected concentrations was found promising, and the coatings were applied to L. acidophilus LA3 and B. longum BB536, using hot-melt fluid bed coating. Viability in and release from the coated probiotic granulate was investigated, using a physiological relevant in vitro model simulating conditions in the human stomach, duodenum, jejunum and ileum. L. acidophilus LA3 coated with cetostearyl alcohol and olive oil in the ratio 95:5 (w/w) resulted in significantly higher viability after simulated gastrointestinal transit, compared to the uncoated probiotic powder. Furthermore, the coating showed no release of viable cells after simulated gastric transit. Released viable cells were detected after the remaining steps in the in vitro model, indicating that the coating system provides gastric protection and release during intestinal transit.",
keywords = "Fluid bed, Hot-melt coating, In vitro simulation, Probiotic delivery systems, Probiotics",
author = "Jacobsen, {Natashia Mai Yde} and Nedergaard, {Hanne Bj{\o}rn} and Anette Kock and Ibrahim Caglayan and Laursen, {Marie Munch} and Eva-Marie Lange and Marcial-Coba, {Mart{\'i}n Sebasti{\'a}n} and Daniel Bar-Shalom and Nielsen, {Dennis Sandris} and Anette M{\"u}llertz",
year = "2021",
doi = "10.1016/j.lwt.2021.111174",
language = "English",
volume = "144",
journal = "Lebensmittel - Wissenschaft und Technologie",
issn = "0023-6438",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit

AU - Jacobsen, Natashia Mai Yde

AU - Nedergaard, Hanne Bjørn

AU - Kock, Anette

AU - Caglayan, Ibrahim

AU - Laursen, Marie Munch

AU - Lange, Eva-Marie

AU - Marcial-Coba, Martín Sebastián

AU - Bar-Shalom, Daniel

AU - Nielsen, Dennis Sandris

AU - Müllertz, Anette

PY - 2021

Y1 - 2021

N2 - Probiotics have become one of the most consumed food supplements worldwide. Many probiotic strains are sensitive to low pH and bile concentrations encountered in the gastrointestinal tract upon oral ingestion. This study aimed at developing gastro-resistant probiotic granulates releasing viable cells in the small intestine. Fatty alcohols were investigated as potential coating materials. Cetostearyl alcohol was selected and combined with different plasticizers to further optimize the coating properties. Combining cetostearyl alcohol with olive oil and beeswax in selected concentrations was found promising, and the coatings were applied to L. acidophilus LA3 and B. longum BB536, using hot-melt fluid bed coating. Viability in and release from the coated probiotic granulate was investigated, using a physiological relevant in vitro model simulating conditions in the human stomach, duodenum, jejunum and ileum. L. acidophilus LA3 coated with cetostearyl alcohol and olive oil in the ratio 95:5 (w/w) resulted in significantly higher viability after simulated gastrointestinal transit, compared to the uncoated probiotic powder. Furthermore, the coating showed no release of viable cells after simulated gastric transit. Released viable cells were detected after the remaining steps in the in vitro model, indicating that the coating system provides gastric protection and release during intestinal transit.

AB - Probiotics have become one of the most consumed food supplements worldwide. Many probiotic strains are sensitive to low pH and bile concentrations encountered in the gastrointestinal tract upon oral ingestion. This study aimed at developing gastro-resistant probiotic granulates releasing viable cells in the small intestine. Fatty alcohols were investigated as potential coating materials. Cetostearyl alcohol was selected and combined with different plasticizers to further optimize the coating properties. Combining cetostearyl alcohol with olive oil and beeswax in selected concentrations was found promising, and the coatings were applied to L. acidophilus LA3 and B. longum BB536, using hot-melt fluid bed coating. Viability in and release from the coated probiotic granulate was investigated, using a physiological relevant in vitro model simulating conditions in the human stomach, duodenum, jejunum and ileum. L. acidophilus LA3 coated with cetostearyl alcohol and olive oil in the ratio 95:5 (w/w) resulted in significantly higher viability after simulated gastrointestinal transit, compared to the uncoated probiotic powder. Furthermore, the coating showed no release of viable cells after simulated gastric transit. Released viable cells were detected after the remaining steps in the in vitro model, indicating that the coating system provides gastric protection and release during intestinal transit.

KW - Fluid bed

KW - Hot-melt coating

KW - In vitro simulation

KW - Probiotic delivery systems

KW - Probiotics

U2 - 10.1016/j.lwt.2021.111174

DO - 10.1016/j.lwt.2021.111174

M3 - Journal article

AN - SCOPUS:85101987357

VL - 144

JO - Lebensmittel - Wissenschaft und Technologie

JF - Lebensmittel - Wissenschaft und Technologie

SN - 0023-6438

M1 - 111174

ER -

ID: 260034115