Background and Aim: The study was undertaken in order to compare single injection indocyanine green (ICG)-clearances with the steady-state ICG-clearance (ICG_Cl) in patients with cirrhosis in order to assess the most accurate estimate for ICG-clearance and to relate the ICG-clearances to established indicators of liver dysfunction. Methods: Thirty-eight patients (male 29) with cirrhosis (Child–Turcotte class A 8, class B 21, and class C 9) were studied during a hemodynamic investigation. A single injection of ICG was followed by blood samples for 5, 10, 15, and 20 min. The dose/plasma area clearance (Cl_A) and plasma volume · initial slope clearance (Cl_PV) were determined and compared with the steady-state infusion/plasma concentration ratio clearance (ICG_Cl). Results: The Cl_A (310; 214; 502 mL/min) and Cl_PV (294; 164; 481 mL/min) correlated closely with ICG_Cl (243; 120; 383 mL/min [median; interquartile range], R = 0.95–0.98, P < 0.000), but were significantly higher than ICG_Cl (P < 0.001). All three clearance measures correlated significantly with biochemical and hemodynamic variables of liver dysfunction (P < 0.05–0.000). All three ICG-clearances showed significantly lower values in patients with ascites compared to those without, and lower ICG-clearance values were present in patients with esophageal varices compared to those without (P < 0.05–0.002). Conclusion: Single injection markers (Cl_A and Cl_PV) of the steady-state ICG-clearance as derived from the ICG-retention curve and the plasma volume correlate with ICG_Cl and established variables of portal hypertension and liver cell bile excretory dysfunction. Therefore, these markers can safely replace the more costly ICG_Cl.