Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method. / Hansen, Åse Marie; Poulsen, O M; Christensen, J M; Hansen, S H.
In: Journal of Chromatography A, Vol. 578, No. 1, 01.07.1992, p. 85-90.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method
AU - Hansen, Åse Marie
AU - Poulsen, O M
AU - Christensen, J M
AU - Hansen, S H
PY - 1992/7/1
Y1 - 1992/7/1
N2 - A high-performance liquid chromatographic method for the determination of 2-naphthylamine in urine using fluorescence detection was developed. The method validation analysis showed the method to be in analytical control, i.e. the distribution of the difference between the observed and true values of the method evaluation samples did not deviate significantly from the normal distribution. The recovery of the method was 85%. The entire run time of chromatography was 10 min using isocratic elution (acetonitrile-water, 35:65), and the retention time for 2-naphthylamine was 5.8 min. The relative short time of analysis in combination with the low limit of detection (0.272 nmol/l) makes the method potentially applicable for surveillance of occupational and environmental exposure to 2-nitronaphthalene. The developed method is presently used for measurement of 2-naphthylamine in urine samples from workers employed at factories, characterized by a low airborne exposure level of polycyclic aromatic hydrocarbons, i.e. in general less than 25 micrograms/m3. The urine samples of exposed workers (n = 95) showed a 2-naphthylamine range of up to 9.4 nmol/l, whereas unexposed control individuals (n = 114) showed a range of up to 0.87 nmol/l.
AB - A high-performance liquid chromatographic method for the determination of 2-naphthylamine in urine using fluorescence detection was developed. The method validation analysis showed the method to be in analytical control, i.e. the distribution of the difference between the observed and true values of the method evaluation samples did not deviate significantly from the normal distribution. The recovery of the method was 85%. The entire run time of chromatography was 10 min using isocratic elution (acetonitrile-water, 35:65), and the retention time for 2-naphthylamine was 5.8 min. The relative short time of analysis in combination with the low limit of detection (0.272 nmol/l) makes the method potentially applicable for surveillance of occupational and environmental exposure to 2-nitronaphthalene. The developed method is presently used for measurement of 2-naphthylamine in urine samples from workers employed at factories, characterized by a low airborne exposure level of polycyclic aromatic hydrocarbons, i.e. in general less than 25 micrograms/m3. The urine samples of exposed workers (n = 95) showed a 2-naphthylamine range of up to 9.4 nmol/l, whereas unexposed control individuals (n = 114) showed a range of up to 0.87 nmol/l.
KW - 2-Naphthylamine
KW - Air Pollutants, Occupational
KW - Chromatography, High Pressure Liquid
KW - Environmental Exposure
KW - Humans
KW - Reproducibility of Results
M3 - Journal article
C2 - 1400790
VL - 578
SP - 85
EP - 90
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0301-4770
IS - 1
ER -
ID: 48866045