Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method. / Hansen, Åse Marie; Poulsen, O M; Christensen, J M; Hansen, S H.

In: Journal of Chromatography A, Vol. 578, No. 1, 01.07.1992, p. 85-90.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hansen, ÅM, Poulsen, OM, Christensen, JM & Hansen, SH 1992, 'Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method', Journal of Chromatography A, vol. 578, no. 1, pp. 85-90.

APA

Hansen, Å. M., Poulsen, O. M., Christensen, J. M., & Hansen, S. H. (1992). Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method. Journal of Chromatography A, 578(1), 85-90.

Vancouver

Hansen ÅM, Poulsen OM, Christensen JM, Hansen SH. Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method. Journal of Chromatography A. 1992 Jul 1;578(1):85-90.

Author

Hansen, Åse Marie ; Poulsen, O M ; Christensen, J M ; Hansen, S H. / Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method. In: Journal of Chromatography A. 1992 ; Vol. 578, No. 1. pp. 85-90.

Bibtex

@article{b14ffdfec11b4c02ab7341263a771937,
title = "Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method",
abstract = "A high-performance liquid chromatographic method for the determination of 2-naphthylamine in urine using fluorescence detection was developed. The method validation analysis showed the method to be in analytical control, i.e. the distribution of the difference between the observed and true values of the method evaluation samples did not deviate significantly from the normal distribution. The recovery of the method was 85%. The entire run time of chromatography was 10 min using isocratic elution (acetonitrile-water, 35:65), and the retention time for 2-naphthylamine was 5.8 min. The relative short time of analysis in combination with the low limit of detection (0.272 nmol/l) makes the method potentially applicable for surveillance of occupational and environmental exposure to 2-nitronaphthalene. The developed method is presently used for measurement of 2-naphthylamine in urine samples from workers employed at factories, characterized by a low airborne exposure level of polycyclic aromatic hydrocarbons, i.e. in general less than 25 micrograms/m3. The urine samples of exposed workers (n = 95) showed a 2-naphthylamine range of up to 9.4 nmol/l, whereas unexposed control individuals (n = 114) showed a range of up to 0.87 nmol/l.",
keywords = "2-Naphthylamine, Air Pollutants, Occupational, Chromatography, High Pressure Liquid, Environmental Exposure, Humans, Reproducibility of Results",
author = "Hansen, {{\AA}se Marie} and Poulsen, {O M} and Christensen, {J M} and Hansen, {S H}",
year = "1992",
month = jul,
day = "1",
language = "English",
volume = "578",
pages = "85--90",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Determination of 2-naphthylamine in urine by a novel reversed-phase high-performance liquid chromatography method

AU - Hansen, Åse Marie

AU - Poulsen, O M

AU - Christensen, J M

AU - Hansen, S H

PY - 1992/7/1

Y1 - 1992/7/1

N2 - A high-performance liquid chromatographic method for the determination of 2-naphthylamine in urine using fluorescence detection was developed. The method validation analysis showed the method to be in analytical control, i.e. the distribution of the difference between the observed and true values of the method evaluation samples did not deviate significantly from the normal distribution. The recovery of the method was 85%. The entire run time of chromatography was 10 min using isocratic elution (acetonitrile-water, 35:65), and the retention time for 2-naphthylamine was 5.8 min. The relative short time of analysis in combination with the low limit of detection (0.272 nmol/l) makes the method potentially applicable for surveillance of occupational and environmental exposure to 2-nitronaphthalene. The developed method is presently used for measurement of 2-naphthylamine in urine samples from workers employed at factories, characterized by a low airborne exposure level of polycyclic aromatic hydrocarbons, i.e. in general less than 25 micrograms/m3. The urine samples of exposed workers (n = 95) showed a 2-naphthylamine range of up to 9.4 nmol/l, whereas unexposed control individuals (n = 114) showed a range of up to 0.87 nmol/l.

AB - A high-performance liquid chromatographic method for the determination of 2-naphthylamine in urine using fluorescence detection was developed. The method validation analysis showed the method to be in analytical control, i.e. the distribution of the difference between the observed and true values of the method evaluation samples did not deviate significantly from the normal distribution. The recovery of the method was 85%. The entire run time of chromatography was 10 min using isocratic elution (acetonitrile-water, 35:65), and the retention time for 2-naphthylamine was 5.8 min. The relative short time of analysis in combination with the low limit of detection (0.272 nmol/l) makes the method potentially applicable for surveillance of occupational and environmental exposure to 2-nitronaphthalene. The developed method is presently used for measurement of 2-naphthylamine in urine samples from workers employed at factories, characterized by a low airborne exposure level of polycyclic aromatic hydrocarbons, i.e. in general less than 25 micrograms/m3. The urine samples of exposed workers (n = 95) showed a 2-naphthylamine range of up to 9.4 nmol/l, whereas unexposed control individuals (n = 114) showed a range of up to 0.87 nmol/l.

KW - 2-Naphthylamine

KW - Air Pollutants, Occupational

KW - Chromatography, High Pressure Liquid

KW - Environmental Exposure

KW - Humans

KW - Reproducibility of Results

M3 - Journal article

C2 - 1400790

VL - 578

SP - 85

EP - 90

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1

ER -

ID: 48866045