Determination of 1-hydroxypyrene in human urine by high-performance liquid chromatography

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Standard

Determination of 1-hydroxypyrene in human urine by high-performance liquid chromatography. / Hansen, Åse Marie; Poulsen, O M; Christensen, J M; Hansen, S H.

In: Journal of Analytical Toxicology, Vol. 17, No. 1, 1993, p. 38-41.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, ÅM, Poulsen, OM, Christensen, JM & Hansen, SH 1993, 'Determination of 1-hydroxypyrene in human urine by high-performance liquid chromatography', Journal of Analytical Toxicology, vol. 17, no. 1, pp. 38-41.

APA

Hansen, Å. M., Poulsen, O. M., Christensen, J. M., & Hansen, S. H. (1993). Determination of 1-hydroxypyrene in human urine by high-performance liquid chromatography. Journal of Analytical Toxicology, 17(1), 38-41.

Vancouver

Hansen ÅM, Poulsen OM, Christensen JM, Hansen SH. Determination of 1-hydroxypyrene in human urine by high-performance liquid chromatography. Journal of Analytical Toxicology. 1993;17(1):38-41.

Author

Hansen, Åse Marie ; Poulsen, O M ; Christensen, J M ; Hansen, S H. / Determination of 1-hydroxypyrene in human urine by high-performance liquid chromatography. In: Journal of Analytical Toxicology. 1993 ; Vol. 17, No. 1. pp. 38-41.

Bibtex

@article{d4216eeb24e3460db10c3ae3d937ee9f,
title = "Determination of 1-hydroxypyrene in human urine by high-performance liquid chromatography",
abstract = "A high-performance liquid chromatography (HPLC)/fluorescence method for quantitative analysis of 1-hydroxypyrene in urine was developed. The method validation analysis showed the method to be in analytical control. No significant systematical errors could be demonstrated. The entire run time of chromatography was 10 min using isocratic elution (acetonitrile-water, 70:30), and the retention time for 1-hydroxypyrene was 3.5 min. The short run time in combination with the low limit detection (1.37 nmol/L) makes the method potentially applicable for surveillance of pyrene exposure in work environments. The developed method is presently used for measurement of 1-hydroxypyrene in urine samples from workers exposed to a low airborne level of polycyclic aromatic hydrocarbons, generally less than 25 micrograms/m3. The urine samples of exposed workers (n = 122) showed a range of 1-hydroxypyrene from the limit of detection (LD) up to 39 nmol/L, whereas unexposed control individuals (n = 108) showed a range from LD to 3.3 nmol/L.",
keywords = "Chromatography, High Pressure Liquid, Humans, Mutagens, Occupational Exposure, Pyrenes, Urine",
author = "Hansen, {{\AA}se Marie} and Poulsen, {O M} and Christensen, {J M} and Hansen, {S H}",
year = "1993",
language = "English",
volume = "17",
pages = "38--41",
journal = "Journal of Analytical Toxicology",
issn = "0146-4760",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Determination of 1-hydroxypyrene in human urine by high-performance liquid chromatography

AU - Hansen, Åse Marie

AU - Poulsen, O M

AU - Christensen, J M

AU - Hansen, S H

PY - 1993

Y1 - 1993

N2 - A high-performance liquid chromatography (HPLC)/fluorescence method for quantitative analysis of 1-hydroxypyrene in urine was developed. The method validation analysis showed the method to be in analytical control. No significant systematical errors could be demonstrated. The entire run time of chromatography was 10 min using isocratic elution (acetonitrile-water, 70:30), and the retention time for 1-hydroxypyrene was 3.5 min. The short run time in combination with the low limit detection (1.37 nmol/L) makes the method potentially applicable for surveillance of pyrene exposure in work environments. The developed method is presently used for measurement of 1-hydroxypyrene in urine samples from workers exposed to a low airborne level of polycyclic aromatic hydrocarbons, generally less than 25 micrograms/m3. The urine samples of exposed workers (n = 122) showed a range of 1-hydroxypyrene from the limit of detection (LD) up to 39 nmol/L, whereas unexposed control individuals (n = 108) showed a range from LD to 3.3 nmol/L.

AB - A high-performance liquid chromatography (HPLC)/fluorescence method for quantitative analysis of 1-hydroxypyrene in urine was developed. The method validation analysis showed the method to be in analytical control. No significant systematical errors could be demonstrated. The entire run time of chromatography was 10 min using isocratic elution (acetonitrile-water, 70:30), and the retention time for 1-hydroxypyrene was 3.5 min. The short run time in combination with the low limit detection (1.37 nmol/L) makes the method potentially applicable for surveillance of pyrene exposure in work environments. The developed method is presently used for measurement of 1-hydroxypyrene in urine samples from workers exposed to a low airborne level of polycyclic aromatic hydrocarbons, generally less than 25 micrograms/m3. The urine samples of exposed workers (n = 122) showed a range of 1-hydroxypyrene from the limit of detection (LD) up to 39 nmol/L, whereas unexposed control individuals (n = 108) showed a range from LD to 3.3 nmol/L.

KW - Chromatography, High Pressure Liquid

KW - Humans

KW - Mutagens

KW - Occupational Exposure

KW - Pyrenes

KW - Urine

M3 - Journal article

C2 - 8429626

VL - 17

SP - 38

EP - 41

JO - Journal of Analytical Toxicology

JF - Journal of Analytical Toxicology

SN - 0146-4760

IS - 1

ER -

ID: 48865986