Demonstration of circulating immune complexes by the indirect leucocyte phagocytosis test in chronic inflammatory bowel disease. Relation to results of a standard complement consumption assay
Research output: Contribution to journal › Journal article › peer-review
Circulating immune complexes were studied in untreated Crohn's disease (CD) and ulcerative colitis (UC) by leucocyte phagocytosis. Neutrophils from normal donors took up large immunoglobulin-containing inclusions from 14 of 15 CD sera, 3 of 15 UC sera (p less than 0.002) and from none of 15 reference sera from healthy volunteers (p less than 0.002). In contrast, inclusions could not be demonstrated on direct microscopic investigation. Our study confirms the presence of circulating immune complexes in Crohn's disease. Predominance of IgG-containing complexes in this condition is consistent with a mucosal origin. Discrepant results obtained by direct examination and by incubation of sera from patients with normal test neutrophils suggest a defective immune complex phagocytosis in CD. In consistency with this possibility, control experiments revealed a markedly decreased complex uptake by neutrophils of CD patients in vitro.
Original language | English |
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Journal | Acta Medica Scandinavica |
Volume | 218 |
Issue number | 1 |
Pages (from-to) | 73-78 |
Number of pages | 6 |
ISSN | 0955-7873 |
DOIs | |
Publication status | Published - 1985 |
Externally published | Yes |
- Antigen-Antibody Complex/analysis, Colitis, Ulcerative/immunology, Complement C3/analysis, Crohn Disease/immunology, Humans, Immunoglobulins/analysis, Lymphocyte Culture Test, Mixed, Neutrophils/immunology, Phagocytosis
Research areas
ID: 218731109