Demonstration of circulating immune complexes by the indirect leucocyte phagocytosis test in chronic inflammatory bowel disease. Relation to results of a standard complement consumption assay
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Demonstration of circulating immune complexes by the indirect leucocyte phagocytosis test in chronic inflammatory bowel disease. Relation to results of a standard complement consumption assay. / Elmgreen, J; Wiik, A; Nielsen, H; Nielsen, O H.
In: Acta Medica Scandinavica, Vol. 218, No. 1, 1985, p. 73-78.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Demonstration of circulating immune complexes by the indirect leucocyte phagocytosis test in chronic inflammatory bowel disease. Relation to results of a standard complement consumption assay
AU - Elmgreen, J
AU - Wiik, A
AU - Nielsen, H
AU - Nielsen, O H
PY - 1985
Y1 - 1985
N2 - Circulating immune complexes were studied in untreated Crohn's disease (CD) and ulcerative colitis (UC) by leucocyte phagocytosis. Neutrophils from normal donors took up large immunoglobulin-containing inclusions from 14 of 15 CD sera, 3 of 15 UC sera (p less than 0.002) and from none of 15 reference sera from healthy volunteers (p less than 0.002). In contrast, inclusions could not be demonstrated on direct microscopic investigation. Our study confirms the presence of circulating immune complexes in Crohn's disease. Predominance of IgG-containing complexes in this condition is consistent with a mucosal origin. Discrepant results obtained by direct examination and by incubation of sera from patients with normal test neutrophils suggest a defective immune complex phagocytosis in CD. In consistency with this possibility, control experiments revealed a markedly decreased complex uptake by neutrophils of CD patients in vitro.
AB - Circulating immune complexes were studied in untreated Crohn's disease (CD) and ulcerative colitis (UC) by leucocyte phagocytosis. Neutrophils from normal donors took up large immunoglobulin-containing inclusions from 14 of 15 CD sera, 3 of 15 UC sera (p less than 0.002) and from none of 15 reference sera from healthy volunteers (p less than 0.002). In contrast, inclusions could not be demonstrated on direct microscopic investigation. Our study confirms the presence of circulating immune complexes in Crohn's disease. Predominance of IgG-containing complexes in this condition is consistent with a mucosal origin. Discrepant results obtained by direct examination and by incubation of sera from patients with normal test neutrophils suggest a defective immune complex phagocytosis in CD. In consistency with this possibility, control experiments revealed a markedly decreased complex uptake by neutrophils of CD patients in vitro.
KW - Antigen-Antibody Complex/analysis
KW - Colitis, Ulcerative/immunology
KW - Complement C3/analysis
KW - Crohn Disease/immunology
KW - Humans
KW - Immunoglobulins/analysis
KW - Lymphocyte Culture Test, Mixed
KW - Neutrophils/immunology
KW - Phagocytosis
U2 - 10.1111/j.0954-6820.1985.tb08828.x.
DO - 10.1111/j.0954-6820.1985.tb08828.x.
M3 - Journal article
C2 - 2931950
VL - 218
SP - 73
EP - 78
JO - Acta Medica Scandinavica
JF - Acta Medica Scandinavica
SN - 0955-7873
IS - 1
ER -
ID: 218731109