Decoding signalling networks by mass spectrometry-based proteomics
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Decoding signalling networks by mass spectrometry-based proteomics. / Choudhary, Chuna Ram; Mann, Matthias.
In: Nature Reviews. Molecular Cell Biology, Vol. 11, No. 6, 01.06.2010, p. 427-39.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Decoding signalling networks by mass spectrometry-based proteomics
AU - Choudhary, Chuna Ram
AU - Mann, Matthias
PY - 2010/6/1
Y1 - 2010/6/1
N2 - Signalling networks regulate essentially all of the biology of cells and organisms in normal and disease states. Signalling is often studied using antibody-based techniques such as western blots. Large-scale 'precision proteomics' based on mass spectrometry now enables the system-wide characterization of signalling events at the levels of post-translational modifications, protein-protein interactions and changes in protein expression. This technology delivers accurate and unbiased information about the quantitative changes of thousands of proteins and their modifications in response to any perturbation. Current studies focus on phosphorylation, but acetylation, methylation, glycosylation and ubiquitylation are also becoming amenable to investigation. Large-scale proteomics-based signalling research will fundamentally change our understanding of signalling networks.
AB - Signalling networks regulate essentially all of the biology of cells and organisms in normal and disease states. Signalling is often studied using antibody-based techniques such as western blots. Large-scale 'precision proteomics' based on mass spectrometry now enables the system-wide characterization of signalling events at the levels of post-translational modifications, protein-protein interactions and changes in protein expression. This technology delivers accurate and unbiased information about the quantitative changes of thousands of proteins and their modifications in response to any perturbation. Current studies focus on phosphorylation, but acetylation, methylation, glycosylation and ubiquitylation are also becoming amenable to investigation. Large-scale proteomics-based signalling research will fundamentally change our understanding of signalling networks.
KW - Animals
KW - Humans
KW - Mass Spectrometry
KW - Protein Processing, Post-Translational
KW - Proteome
KW - Proteomics
KW - Signal Transduction
U2 - 10.1038/nrm2900
DO - 10.1038/nrm2900
M3 - Journal article
C2 - 20461098
VL - 11
SP - 427
EP - 439
JO - Nature Reviews. Molecular Cell Biology
JF - Nature Reviews. Molecular Cell Biology
SN - 1471-0072
IS - 6
ER -
ID: 33748656