DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes
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- cts.12768
Final published version, 661 KB, PDF document
Contamination is a potential problem in the study of ancient proteins, either from prior handling of the sample, laboratory consumables, or cross-sample carryover from mass spectrometers. Recently, deamidation of glutamine has been proposed as a measure for assessing the degradation of ancient proteins. Here, we present deamiDATE 1.0, a method for the authentication of ancient proteins using measure of site-specific deamidation rates. We test this approach on shotgun proteomic data derived from bone collagen from modern, archaeological and extinct taxa. We further demonstrate how this method may be used to differentiate between modern contaminants and authentic ancient proteins using a case study from Neolithic dental calculus.
Original language | English |
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Article number | 105080 |
Journal | Journal of Archaeological Science |
Volume | 115 |
Number of pages | 10 |
ISSN | 0305-4403 |
DOIs | |
Publication status | Published - Mar 2020 |
- Authentication, Bioinformatics, Contamination, Palaeoproteomics
Research areas
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