DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes
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DeamiDATE 1.0 : Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes. / Ramsøe, Abigail; van Heekeren, Vivian; Ponce, Paola; Fischer, Roman; Barnes, Ian; Speller, Camilla; Collins, Matthew J.
In: Journal of Archaeological Science, Vol. 115, 105080, 03.2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - DeamiDATE 1.0
T2 - Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes
AU - Ramsøe, Abigail
AU - van Heekeren, Vivian
AU - Ponce, Paola
AU - Fischer, Roman
AU - Barnes, Ian
AU - Speller, Camilla
AU - Collins, Matthew J.
PY - 2020/3
Y1 - 2020/3
N2 - Contamination is a potential problem in the study of ancient proteins, either from prior handling of the sample, laboratory consumables, or cross-sample carryover from mass spectrometers. Recently, deamidation of glutamine has been proposed as a measure for assessing the degradation of ancient proteins. Here, we present deamiDATE 1.0, a method for the authentication of ancient proteins using measure of site-specific deamidation rates. We test this approach on shotgun proteomic data derived from bone collagen from modern, archaeological and extinct taxa. We further demonstrate how this method may be used to differentiate between modern contaminants and authentic ancient proteins using a case study from Neolithic dental calculus.
AB - Contamination is a potential problem in the study of ancient proteins, either from prior handling of the sample, laboratory consumables, or cross-sample carryover from mass spectrometers. Recently, deamidation of glutamine has been proposed as a measure for assessing the degradation of ancient proteins. Here, we present deamiDATE 1.0, a method for the authentication of ancient proteins using measure of site-specific deamidation rates. We test this approach on shotgun proteomic data derived from bone collagen from modern, archaeological and extinct taxa. We further demonstrate how this method may be used to differentiate between modern contaminants and authentic ancient proteins using a case study from Neolithic dental calculus.
KW - Authentication
KW - Bioinformatics
KW - Contamination
KW - Palaeoproteomics
U2 - 10.1016/j.jas.2020.105080
DO - 10.1016/j.jas.2020.105080
M3 - Journal article
AN - SCOPUS:85078191602
VL - 115
JO - Journal of Archaeological Science
JF - Journal of Archaeological Science
SN - 0305-4403
M1 - 105080
ER -
ID: 241988791