Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.
|Title of host publication||P-Type ATPases : Methods and Protocols|
|Number of pages||8|
|Publication status||Published - 2016|
|Series||Methods in Molecular Biology|