Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method

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Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.

Original languageEnglish
Title of host publicationP-Type ATPases : Methods and Protocols
EditorsMaike Bublitz
Number of pages8
Publication date2016
ISBN (Print)978-1-4939-3178-1
ISBN (Electronic)978-1-4939-3179-8
Publication statusPublished - 2016
SeriesMethods in Molecular Biology

ID: 174864931