Crystallization of a stringent response factor from Aquifex aeolicus
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Crystallization of a stringent response factor from Aquifex aeolicus. / Kristensen, Ole; Laurberg, Martin; Gajhede, Michael.
In: Acta Crystallographica. Section D: Biological Crystallography, Vol. 58, No. Pt 7, 2002, p. 1198-200.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Crystallization of a stringent response factor from Aquifex aeolicus
AU - Kristensen, Ole
AU - Laurberg, Martin
AU - Gajhede, Michael
PY - 2002
Y1 - 2002
N2 - The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.
AB - The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.
KW - Bacteria
KW - Chromatography, Gel
KW - Cloning, Molecular
KW - Crystallography, X-Ray
KW - Dimerization
KW - Disulfides
KW - Electrophoresis, Polyacrylamide Gel
KW - Hydrogen Peroxide
KW - Mutation
KW - Phosphoprotein Phosphatases
KW - Polymerase Chain Reaction
KW - Software
M3 - Journal article
C2 - 12077442
VL - 58
SP - 1198
EP - 1200
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
SN - 2059-7983
IS - Pt 7
ER -
ID: 40318741