Contribution of volatiles to the antifungal effect of Lactobacillus paracasei in defined medium and yogurt
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Contribution of volatiles to the antifungal effect of Lactobacillus paracasei in defined medium and yogurt. / Aunsbjerg, Stina Dissing; Honoré, Anders Hans; Marcussen, J.; Ebrahimi, Parvaneh; Vogensen, Finn Kvist; Benfeldt, C.; Skov, Thomas; Knøchel, Susanne.
In: International Journal of Food Microbiology, Vol. 194, 2015, p. 46-53.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Contribution of volatiles to the antifungal effect of Lactobacillus paracasei in defined medium and yogurt
AU - Aunsbjerg, Stina Dissing
AU - Honoré, Anders Hans
AU - Marcussen, J.
AU - Ebrahimi, Parvaneh
AU - Vogensen, Finn Kvist
AU - Benfeldt, C.
AU - Skov, Thomas
AU - Knøchel, Susanne
N1 - Copyright © 2014 Elsevier B.V. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Lactic acid bacteria with antifungal properties can be used to control spoilage of food and feed. Previously, most of the identified metabolites have been isolated from cell-free fermentate of lactic acid bacteria with methods suboptimal for detecting possible contribution from volatiles to the antifungal activity. The role of volatile compounds in the antifungal activity of Lactobacillus paracasei DGCC 2132 in a chemically defined interaction medium (CDIM) and yogurt was therefore investigated with a sampling technique minimizing volatile loss. Diacetyl was identified as the major volatile produced by L. paracasei DGCC 2132 in CDIM. When the strain was added to a yogurt medium diacetyl as well as other volatiles also increased but the metabolome was more complex. Removal of L. paracasei DGCC 2132 cells from CDIM fermentate resulted in loss of both volatiles, including diacetyl, and the antifungal activity towards two strains of Penicillium spp. When adding diacetyl to CDIM or yogurt without L. paracasei DGCC 2132, marked inhibition was observed. Besides diacetyl, the antifungal properties of acetoin were examined, but no antifungal activity was observed. Overall, the results demonstrate the contribution of diacetyl in the antifungal effect of L. paracasei DGCC 2132 and indicate that the importance of volatiles may have been previously underestimated.
AB - Lactic acid bacteria with antifungal properties can be used to control spoilage of food and feed. Previously, most of the identified metabolites have been isolated from cell-free fermentate of lactic acid bacteria with methods suboptimal for detecting possible contribution from volatiles to the antifungal activity. The role of volatile compounds in the antifungal activity of Lactobacillus paracasei DGCC 2132 in a chemically defined interaction medium (CDIM) and yogurt was therefore investigated with a sampling technique minimizing volatile loss. Diacetyl was identified as the major volatile produced by L. paracasei DGCC 2132 in CDIM. When the strain was added to a yogurt medium diacetyl as well as other volatiles also increased but the metabolome was more complex. Removal of L. paracasei DGCC 2132 cells from CDIM fermentate resulted in loss of both volatiles, including diacetyl, and the antifungal activity towards two strains of Penicillium spp. When adding diacetyl to CDIM or yogurt without L. paracasei DGCC 2132, marked inhibition was observed. Besides diacetyl, the antifungal properties of acetoin were examined, but no antifungal activity was observed. Overall, the results demonstrate the contribution of diacetyl in the antifungal effect of L. paracasei DGCC 2132 and indicate that the importance of volatiles may have been previously underestimated.
U2 - 10.1016/j.ijfoodmicro.2014.11.007
DO - 10.1016/j.ijfoodmicro.2014.11.007
M3 - Journal article
C2 - 25461608
VL - 194
SP - 46
EP - 53
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
SN - 0168-1605
ER -
ID: 130208121