Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G

Research output: Contribution to journal › Journal article › Research › peer-review

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Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G. / Clark, Angela T.; Robertson, Morwenna E.M.; Conn, Graeme L.; Belsham, Graham J.

In: Journal of Virology, Vol. 77, No. 23, 12.2003, p. 12441-12449.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Clark, AT, Robertson, MEM, Conn, GL & Belsham, GJ 2003, 'Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G', Journal of Virology, vol. 77, no. 23, pp. 12441-12449. https://doi.org/10.1128/JVI.77.23.12441-12449.2003

APA

Clark, A. T., Robertson, M. E. M., Conn, G. L., & Belsham, G. J. (2003). Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G. Journal of Virology, 77(23), 12441-12449. https://doi.org/10.1128/JVI.77.23.12441-12449.2003

Vancouver

Clark AT, Robertson MEM, Conn GL, Belsham GJ. Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G. Journal of Virology. 2003 Dec;77(23):12441-12449. https://doi.org/10.1128/JVI.77.23.12441-12449.2003

Author

Clark, Angela T. ; Robertson, Morwenna E.M. ; Conn, Graeme L. ; Belsham, Graham J. / Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G. In: Journal of Virology. 2003 ; Vol. 77, No. 23. pp. 12441-12449.

Bibtex

@article{9bf4c8d0ff224169809a2e665e198512,
title = "Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G",
abstract = "The internal ribosome entry site (IRES) elements of cardioviruses (e.g., encephalomyocarditis virus [EMCV] and foot-and-mouth disease virus) are predicted to have very similar secondary structures. Among these complex RNA structures there is only rather limited complete sequence conservation. Within the J domain of the EMCV IRES there are four highly conserved nucleotides (A704, C705, G723, and A724)., which are predicted to be unpaired and have been targeted for mutagenesis. Using an IRES-dependent cell selection system, we have isolated functional IRES elements from a pool of up to 256 mutants. All changes to these conserved nucleotides resulted in IRES elements that were less efficient at directing internal initiation of translation than the wild-type element, and even some of the single point mutants were highly defective. Each of the mutations adversely affected the ability of the RNAs to interact with the translation initiation factor eIF4G.",
author = "Clark, {Angela T.} and Robertson, {Morwenna E.M.} and Conn, {Graeme L.} and Belsham, {Graham J.}",
year = "2003",
month = dec,
doi = "10.1128/JVI.77.23.12441-12449.2003",
language = "English",
volume = "77",
pages = "12441--12449",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "23",

}

RIS

TY - JOUR

T1 - Conserved Nucleotides within the J Domain of the Encephalomyocarditis Virus Internal Ribosome Entry Site Are Required for Activity and for Interaction with eIF4G

AU - Clark, Angela T.

AU - Robertson, Morwenna E.M.

AU - Conn, Graeme L.

AU - Belsham, Graham J.

PY - 2003/12

Y1 - 2003/12

N2 - The internal ribosome entry site (IRES) elements of cardioviruses (e.g., encephalomyocarditis virus [EMCV] and foot-and-mouth disease virus) are predicted to have very similar secondary structures. Among these complex RNA structures there is only rather limited complete sequence conservation. Within the J domain of the EMCV IRES there are four highly conserved nucleotides (A704, C705, G723, and A724)., which are predicted to be unpaired and have been targeted for mutagenesis. Using an IRES-dependent cell selection system, we have isolated functional IRES elements from a pool of up to 256 mutants. All changes to these conserved nucleotides resulted in IRES elements that were less efficient at directing internal initiation of translation than the wild-type element, and even some of the single point mutants were highly defective. Each of the mutations adversely affected the ability of the RNAs to interact with the translation initiation factor eIF4G.

AB - The internal ribosome entry site (IRES) elements of cardioviruses (e.g., encephalomyocarditis virus [EMCV] and foot-and-mouth disease virus) are predicted to have very similar secondary structures. Among these complex RNA structures there is only rather limited complete sequence conservation. Within the J domain of the EMCV IRES there are four highly conserved nucleotides (A704, C705, G723, and A724)., which are predicted to be unpaired and have been targeted for mutagenesis. Using an IRES-dependent cell selection system, we have isolated functional IRES elements from a pool of up to 256 mutants. All changes to these conserved nucleotides resulted in IRES elements that were less efficient at directing internal initiation of translation than the wild-type element, and even some of the single point mutants were highly defective. Each of the mutations adversely affected the ability of the RNAs to interact with the translation initiation factor eIF4G.

UR - http://www.scopus.com/inward/record.url?scp=0242493466&partnerID=8YFLogxK

U2 - 10.1128/JVI.77.23.12441-12449.2003

DO - 10.1128/JVI.77.23.12441-12449.2003

M3 - Journal article

C2 - 14610168

AN - SCOPUS:0242493466

VL - 77

SP - 12441

EP - 12449

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 23

ER -

ID: 379026546