Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis
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Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis. / Kristiansen, K; Hartmann, B; Plesner, P; Krüger, A.
In: European Journal of Biochemistry, Vol. 83, No. 2, 1978, p. 389-94.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis
AU - Kristiansen, K
AU - Hartmann, B
AU - Plesner, P
AU - Krüger, A
N1 - Keywords: Acetone; Animals; Cell Fractionation; Molecular Weight; Protein Biosynthesis; Ribosomal Proteins; Ribosomes; Tetrahymena pyriformis
PY - 1978
Y1 - 1978
N2 - The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.
AB - The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.
M3 - Journal article
C2 - 415859
VL - 83
SP - 389
EP - 394
JO - FEBS Journal
JF - FEBS Journal
SN - 1742-464X
IS - 2
ER -
ID: 11368600