Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid. / Olesen, Ann Sofie; Lohse, Louise; Dalgaard, Marlene Danner; Woźniakowski, Grzegorz; Belsham, Graham J; Bøtner, Anette; Rasmussen, Thomas Bruun.

In: Journal of Virological Methods, Vol. 261, 11.2018, p. 14-16.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Olesen, AS, Lohse, L, Dalgaard, MD, Woźniakowski, G, Belsham, GJ, Bøtner, A & Rasmussen, TB 2018, 'Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid', Journal of Virological Methods, vol. 261, pp. 14-16. https://doi.org/10.1016/j.jviromet.2018.07.015

APA

Olesen, A. S., Lohse, L., Dalgaard, M. D., Woźniakowski, G., Belsham, G. J., Bøtner, A., & Rasmussen, T. B. (2018). Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid. Journal of Virological Methods, 261, 14-16. https://doi.org/10.1016/j.jviromet.2018.07.015

Vancouver

Olesen AS, Lohse L, Dalgaard MD, Woźniakowski G, Belsham GJ, Bøtner A et al. Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid. Journal of Virological Methods. 2018 Nov;261:14-16. https://doi.org/10.1016/j.jviromet.2018.07.015

Author

Olesen, Ann Sofie ; Lohse, Louise ; Dalgaard, Marlene Danner ; Woźniakowski, Grzegorz ; Belsham, Graham J ; Bøtner, Anette ; Rasmussen, Thomas Bruun. / Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid. In: Journal of Virological Methods. 2018 ; Vol. 261. pp. 14-16.

Bibtex

@article{4feec0e71fc94526b7d99e8c3ace3e34,
title = "Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid",
abstract = "African swine fever (ASF) is an important disease of domestic pigs and wild boar. The disease is caused by African swine fever virus (ASFV). In 2014, ASFV was introduced into Eastern Europe, and it has since then continued to spread within various Eastern European countries. Investigating differences in sequences between ASFV isolates may be a valuable tool to understand differences in virulence among them, however currently, no complete genome sequences of the viruses responsible for the Eastern European outbreaks have been reported. In this study, the complete genome sequence of a highly virulent ASFV was determined directly from erythrocyte-associated nucleic acids obtained from a pig experimentally infected with an isolate from Poland (ASFV POL/2015/Podlaskie). The sequence (ca. 189 kb) of this recent European ASFV showed 95 nt differences (99.95% identity) from the ASFV Georgia 2007/1 genome. The complete sequence of ASFV POL/2015/Podlaskie should assist further studies on the genetic diversity and evolution of the European ASFVs.",
keywords = "African Swine Fever/virology, African Swine Fever Virus/genetics, Animals, DNA, Viral/chemistry, Erythrocytes/virology, Genome, Viral, Poland, Sequence Analysis, DNA, Swine",
author = "Olesen, {Ann Sofie} and Louise Lohse and Dalgaard, {Marlene Danner} and Grzegorz Wo{\'z}niakowski and Belsham, {Graham J} and Anette B{\o}tner and Rasmussen, {Thomas Bruun}",
note = "Copyright {\textcopyright} 2018 Elsevier B.V. All rights reserved.",
year = "2018",
month = nov,
doi = "10.1016/j.jviromet.2018.07.015",
language = "English",
volume = "261",
pages = "14--16",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid

AU - Olesen, Ann Sofie

AU - Lohse, Louise

AU - Dalgaard, Marlene Danner

AU - Woźniakowski, Grzegorz

AU - Belsham, Graham J

AU - Bøtner, Anette

AU - Rasmussen, Thomas Bruun

N1 - Copyright © 2018 Elsevier B.V. All rights reserved.

PY - 2018/11

Y1 - 2018/11

N2 - African swine fever (ASF) is an important disease of domestic pigs and wild boar. The disease is caused by African swine fever virus (ASFV). In 2014, ASFV was introduced into Eastern Europe, and it has since then continued to spread within various Eastern European countries. Investigating differences in sequences between ASFV isolates may be a valuable tool to understand differences in virulence among them, however currently, no complete genome sequences of the viruses responsible for the Eastern European outbreaks have been reported. In this study, the complete genome sequence of a highly virulent ASFV was determined directly from erythrocyte-associated nucleic acids obtained from a pig experimentally infected with an isolate from Poland (ASFV POL/2015/Podlaskie). The sequence (ca. 189 kb) of this recent European ASFV showed 95 nt differences (99.95% identity) from the ASFV Georgia 2007/1 genome. The complete sequence of ASFV POL/2015/Podlaskie should assist further studies on the genetic diversity and evolution of the European ASFVs.

AB - African swine fever (ASF) is an important disease of domestic pigs and wild boar. The disease is caused by African swine fever virus (ASFV). In 2014, ASFV was introduced into Eastern Europe, and it has since then continued to spread within various Eastern European countries. Investigating differences in sequences between ASFV isolates may be a valuable tool to understand differences in virulence among them, however currently, no complete genome sequences of the viruses responsible for the Eastern European outbreaks have been reported. In this study, the complete genome sequence of a highly virulent ASFV was determined directly from erythrocyte-associated nucleic acids obtained from a pig experimentally infected with an isolate from Poland (ASFV POL/2015/Podlaskie). The sequence (ca. 189 kb) of this recent European ASFV showed 95 nt differences (99.95% identity) from the ASFV Georgia 2007/1 genome. The complete sequence of ASFV POL/2015/Podlaskie should assist further studies on the genetic diversity and evolution of the European ASFVs.

KW - African Swine Fever/virology

KW - African Swine Fever Virus/genetics

KW - Animals

KW - DNA, Viral/chemistry

KW - Erythrocytes/virology

KW - Genome, Viral

KW - Poland

KW - Sequence Analysis, DNA

KW - Swine

U2 - 10.1016/j.jviromet.2018.07.015

DO - 10.1016/j.jviromet.2018.07.015

M3 - Journal article

C2 - 30063908

VL - 261

SP - 14

EP - 16

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

ER -

ID: 257914400