Cloning and characterization of Aspergillus niger genes encoding an α-galactosidase and a β-mannosidase involved in galactomannan degradation

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Cloning and characterization of Aspergillus niger genes encoding an α-galactosidase and a β-mannosidase involved in galactomannan degradation. / Ademark, Pia; De Vries, Ronald P.; Hägglund, Per; Stålbrand, Henrik; Visser, Jaap.

In: European Journal of Biochemistry, Vol. 268, No. 10, 27.09.2001, p. 2982-2990.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ademark, P, De Vries, RP, Hägglund, P, Stålbrand, H & Visser, J 2001, 'Cloning and characterization of Aspergillus niger genes encoding an α-galactosidase and a β-mannosidase involved in galactomannan degradation', European Journal of Biochemistry, vol. 268, no. 10, pp. 2982-2990. https://doi.org/10.1046/j.1432-1327.2001.02188.x

APA

Ademark, P., De Vries, R. P., Hägglund, P., Stålbrand, H., & Visser, J. (2001). Cloning and characterization of Aspergillus niger genes encoding an α-galactosidase and a β-mannosidase involved in galactomannan degradation. European Journal of Biochemistry, 268(10), 2982-2990. https://doi.org/10.1046/j.1432-1327.2001.02188.x

Vancouver

Ademark P, De Vries RP, Hägglund P, Stålbrand H, Visser J. Cloning and characterization of Aspergillus niger genes encoding an α-galactosidase and a β-mannosidase involved in galactomannan degradation. European Journal of Biochemistry. 2001 Sep 27;268(10):2982-2990. https://doi.org/10.1046/j.1432-1327.2001.02188.x

Author

Ademark, Pia ; De Vries, Ronald P. ; Hägglund, Per ; Stålbrand, Henrik ; Visser, Jaap. / Cloning and characterization of Aspergillus niger genes encoding an α-galactosidase and a β-mannosidase involved in galactomannan degradation. In: European Journal of Biochemistry. 2001 ; Vol. 268, No. 10. pp. 2982-2990.

Bibtex

@article{832730afe4574df6bcde67c0f0bfa58d,
title = "Cloning and characterization of Aspergillus niger genes encoding an α-galactosidase and a β-mannosidase involved in galactomannan degradation",
abstract = "α-Galactosidase (EC 3.2.1.22) and β-mannosidase (EC 3.2.1.25) participate in the hydrolysis of complex plant saccharides such as galacto(gluco)mannans. Here we report on the cloning and characterization of genes encoding an α-galactosidase (AglC) and a β-mannosidase (MndA) from Aspergillus niger. The aglC and mndA genes code for 747 and 931 amino acids, respectively, including the eukaryotic signal sequences. The predicted isoelectric points of AglC and MndA are 4.56 and 5.17, and the calculated molecular masses are 79.674 and 102.335 kDa, respectively. Both AglC and MndA contain several putative N-glycosylation sites. AglC was assigned to family 36 of the glycosyl hydrolases and MndA was assigned to family 2. The expression patterns of aglC and mndA and two other genes encoding A. niger α-galactosidases (aglA and aglB) during cultivation on galactomannan were studied by Northern analysis. A comparison of gene expression on monosaccharides in the A. niger wild-type and a CreA mutant strain showed that the carbon catabolite repressor protein CreA has a strong influence on aglA, but not on aglB, aglC or mndA. AglC and MndA were purified from constructed overexpression strains of A. niger, and the combined action of these enzymes degraded a galactomanno-oligosaccharide into galactose and mannose. The possible roles of AglC and MndA in galactomannan hydrolysis is discussed.",
keywords = "α-galactosidase, β-mannosidase, Aspergillus niger, Cloning, Expression",
author = "Pia Ademark and {De Vries}, {Ronald P.} and Per H{\"a}gglund and Henrik St{\aa}lbrand and Jaap Visser",
year = "2001",
month = sep,
day = "27",
doi = "10.1046/j.1432-1327.2001.02188.x",
language = "English",
volume = "268",
pages = "2982--2990",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Springer Verlag",
number = "10",

}

RIS

TY - JOUR

T1 - Cloning and characterization of Aspergillus niger genes encoding an α-galactosidase and a β-mannosidase involved in galactomannan degradation

AU - Ademark, Pia

AU - De Vries, Ronald P.

AU - Hägglund, Per

AU - Stålbrand, Henrik

AU - Visser, Jaap

PY - 2001/9/27

Y1 - 2001/9/27

N2 - α-Galactosidase (EC 3.2.1.22) and β-mannosidase (EC 3.2.1.25) participate in the hydrolysis of complex plant saccharides such as galacto(gluco)mannans. Here we report on the cloning and characterization of genes encoding an α-galactosidase (AglC) and a β-mannosidase (MndA) from Aspergillus niger. The aglC and mndA genes code for 747 and 931 amino acids, respectively, including the eukaryotic signal sequences. The predicted isoelectric points of AglC and MndA are 4.56 and 5.17, and the calculated molecular masses are 79.674 and 102.335 kDa, respectively. Both AglC and MndA contain several putative N-glycosylation sites. AglC was assigned to family 36 of the glycosyl hydrolases and MndA was assigned to family 2. The expression patterns of aglC and mndA and two other genes encoding A. niger α-galactosidases (aglA and aglB) during cultivation on galactomannan were studied by Northern analysis. A comparison of gene expression on monosaccharides in the A. niger wild-type and a CreA mutant strain showed that the carbon catabolite repressor protein CreA has a strong influence on aglA, but not on aglB, aglC or mndA. AglC and MndA were purified from constructed overexpression strains of A. niger, and the combined action of these enzymes degraded a galactomanno-oligosaccharide into galactose and mannose. The possible roles of AglC and MndA in galactomannan hydrolysis is discussed.

AB - α-Galactosidase (EC 3.2.1.22) and β-mannosidase (EC 3.2.1.25) participate in the hydrolysis of complex plant saccharides such as galacto(gluco)mannans. Here we report on the cloning and characterization of genes encoding an α-galactosidase (AglC) and a β-mannosidase (MndA) from Aspergillus niger. The aglC and mndA genes code for 747 and 931 amino acids, respectively, including the eukaryotic signal sequences. The predicted isoelectric points of AglC and MndA are 4.56 and 5.17, and the calculated molecular masses are 79.674 and 102.335 kDa, respectively. Both AglC and MndA contain several putative N-glycosylation sites. AglC was assigned to family 36 of the glycosyl hydrolases and MndA was assigned to family 2. The expression patterns of aglC and mndA and two other genes encoding A. niger α-galactosidases (aglA and aglB) during cultivation on galactomannan were studied by Northern analysis. A comparison of gene expression on monosaccharides in the A. niger wild-type and a CreA mutant strain showed that the carbon catabolite repressor protein CreA has a strong influence on aglA, but not on aglB, aglC or mndA. AglC and MndA were purified from constructed overexpression strains of A. niger, and the combined action of these enzymes degraded a galactomanno-oligosaccharide into galactose and mannose. The possible roles of AglC and MndA in galactomannan hydrolysis is discussed.

KW - α-galactosidase

KW - β-mannosidase

KW - Aspergillus niger

KW - Cloning

KW - Expression

UR - http://www.scopus.com/inward/record.url?scp=0034819282&partnerID=8YFLogxK

U2 - 10.1046/j.1432-1327.2001.02188.x

DO - 10.1046/j.1432-1327.2001.02188.x

M3 - Journal article

C2 - 11358516

AN - SCOPUS:0034819282

VL - 268

SP - 2982

EP - 2990

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 10

ER -

ID: 240162427