Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease
Research output: Contribution to journal › Journal article › Research › peer-review
OBJECTIVE: Soluble L-selectin (sL-selectin) concentrations are positively correlated with disease activity in ulcerative colitis (UC) but not in Crohn's disease (CD). This difference in sL-selectin regulation could be due to a disease specific regulation of L-selectin ligands. The aim of this study was to compare levels of circulating sL-selectin, expression of the L-selectin ligand CD34 in the affected colon, and inflammatory bowel disease activity.
METHODS: Twenty-three patients with UC, 16 patients with CD, and 18 control subjects were included in the study. In blood samples concentrations of serum sL-selectin were determined by an ELISA technique. In colonoscopically obtained biopsies, CD34 expression was evaluated by immunohistochemical methods using monoclonal CD34 antibodies. Disease activity was determined by a clinical semiquantitative scale.
RESULTS: sL-selectin levels were found to be significantly increased along with increasing disease activity in UC (p < 0.001) but not in CD (p > 0.05) patients. UC patients with quiescent and severe disease activity had significantly lower (p < 0.005) and higher (p < 0.002) sL-selectin concentrations than controls, respectively. CD34 expression was found to be increased in both disease groups as compared with controls (p < 0.05).
CONCLUSION: A disease-specific regulation of CD34 was not found as an explanation for the distinction in sL-selectin regulation. In the light of recent reports on low sL-selectin in other diseases, it is suggested instead that ongoing neutrophil activation may be the reason for low sL-selectin concentrations during quiescent disease stages, whereas chemokine secretion could explain the increased levels of sL-selectin associated with severe disease activity.
Original language | English |
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Journal | The American Journal of Gastroenterology |
Volume | 93 |
Issue number | 10 |
Pages (from-to) | 1854-9 |
Number of pages | 6 |
ISSN | 0002-9270 |
DOIs | |
Publication status | Published - Oct 1998 |
- Adult, Antigens, CD34, Biopsy, Case-Control Studies, Colitis, Ulcerative, Colon, Crohn Disease, Enzyme-Linked Immunosorbent Assay, Female, Humans, L-Selectin, Male, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 173051741