Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease
Research output: Contribution to journal › Journal article › Research › peer-review
OBJECTIVE: Soluble L-selectin (sL-selectin) concentrations are positively correlated with disease activity in ulcerative colitis (UC) but not in Crohn's disease (CD). This difference in sL-selectin regulation could be due to a disease specific regulation of L-selectin ligands. The aim of this study was to compare levels of circulating sL-selectin, expression of the L-selectin ligand CD34 in the affected colon, and inflammatory bowel disease activity.
METHODS: Twenty-three patients with UC, 16 patients with CD, and 18 control subjects were included in the study. In blood samples concentrations of serum sL-selectin were determined by an ELISA technique. In colonoscopically obtained biopsies, CD34 expression was evaluated by immunohistochemical methods using monoclonal CD34 antibodies. Disease activity was determined by a clinical semiquantitative scale.
RESULTS: sL-selectin levels were found to be significantly increased along with increasing disease activity in UC (p <0.001) (p but cd in not> 0.05) patients. UC patients with quiescent and severe disease activity had significantly lower (p < 0.005) and higher (p < 0.002) sL-selectin concentrations than controls, respectively. CD34 expression was found to be increased in both disease groups as compared with controls (p < 0.05).0.001)>
CONCLUSION: A disease-specific regulation of CD34 was not found as an explanation for the distinction in sL-selectin regulation. In the light of recent reports on low sL-selectin in other diseases, it is suggested instead that ongoing neutrophil activation may be the reason for low sL-selectin concentrations during quiescent disease stages, whereas chemokine secretion could explain the increased levels of sL-selectin associated with severe disease activity.
|Journal||The American Journal of Gastroenterology|
|Number of pages||6|
|Publication status||Published - Oct 1998|
- Adult, Antigens, CD34, Biopsy, Case-Control Studies, Colitis, Ulcerative, Colon, Crohn Disease, Enzyme-Linked Immunosorbent Assay, Female, Humans, L-Selectin, Male, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't