Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease. / Seidelin, J B; Vainer, B; Horn, T; Nielsen, O H.

In: The American Journal of Gastroenterology, Vol. 93, No. 10, 10.1998, p. 1854-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Seidelin, JB, Vainer, B, Horn, T & Nielsen, OH 1998, 'Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease', The American Journal of Gastroenterology, vol. 93, no. 10, pp. 1854-9. https://doi.org/10.1111/j.1572-0241.1998.538_f.x

APA

Seidelin, J. B., Vainer, B., Horn, T., & Nielsen, O. H. (1998). Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease. The American Journal of Gastroenterology, 93(10), 1854-9. https://doi.org/10.1111/j.1572-0241.1998.538_f.x

Vancouver

Seidelin JB, Vainer B, Horn T, Nielsen OH. Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease. The American Journal of Gastroenterology. 1998 Oct;93(10):1854-9. https://doi.org/10.1111/j.1572-0241.1998.538_f.x

Author

Seidelin, J B ; Vainer, B ; Horn, T ; Nielsen, O H. / Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease. In: The American Journal of Gastroenterology. 1998 ; Vol. 93, No. 10. pp. 1854-9.

Bibtex

@article{a50876258db444aa82284a4716f77448,
title = "Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease",
abstract = "OBJECTIVE: Soluble L-selectin (sL-selectin) concentrations are positively correlated with disease activity in ulcerative colitis (UC) but not in Crohn's disease (CD). This difference in sL-selectin regulation could be due to a disease specific regulation of L-selectin ligands. The aim of this study was to compare levels of circulating sL-selectin, expression of the L-selectin ligand CD34 in the affected colon, and inflammatory bowel disease activity.METHODS: Twenty-three patients with UC, 16 patients with CD, and 18 control subjects were included in the study. In blood samples concentrations of serum sL-selectin were determined by an ELISA technique. In colonoscopically obtained biopsies, CD34 expression was evaluated by immunohistochemical methods using monoclonal CD34 antibodies. Disease activity was determined by a clinical semiquantitative scale.RESULTS: sL-selectin levels were found to be significantly increased along with increasing disease activity in UC (p < 0.001) but not in CD (p > 0.05) patients. UC patients with quiescent and severe disease activity had significantly lower (p < 0.005) and higher (p < 0.002) sL-selectin concentrations than controls, respectively. CD34 expression was found to be increased in both disease groups as compared with controls (p < 0.05).CONCLUSION: A disease-specific regulation of CD34 was not found as an explanation for the distinction in sL-selectin regulation. In the light of recent reports on low sL-selectin in other diseases, it is suggested instead that ongoing neutrophil activation may be the reason for low sL-selectin concentrations during quiescent disease stages, whereas chemokine secretion could explain the increased levels of sL-selectin associated with severe disease activity.",
keywords = "Adult, Antigens, CD34, Biopsy, Case-Control Studies, Colitis, Ulcerative, Colon, Crohn Disease, Enzyme-Linked Immunosorbent Assay, Female, Humans, L-Selectin, Male, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",
author = "Seidelin, {J B} and B Vainer and T Horn and Nielsen, {O H}",
year = "1998",
month = oct,
doi = "10.1111/j.1572-0241.1998.538_f.x",
language = "English",
volume = "93",
pages = "1854--9",
journal = "The American Journal of Gastroenterology",
issn = "0002-9270",
publisher = "nature publishing group",
number = "10",

}

RIS

TY - JOUR

T1 - Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease

AU - Seidelin, J B

AU - Vainer, B

AU - Horn, T

AU - Nielsen, O H

PY - 1998/10

Y1 - 1998/10

N2 - OBJECTIVE: Soluble L-selectin (sL-selectin) concentrations are positively correlated with disease activity in ulcerative colitis (UC) but not in Crohn's disease (CD). This difference in sL-selectin regulation could be due to a disease specific regulation of L-selectin ligands. The aim of this study was to compare levels of circulating sL-selectin, expression of the L-selectin ligand CD34 in the affected colon, and inflammatory bowel disease activity.METHODS: Twenty-three patients with UC, 16 patients with CD, and 18 control subjects were included in the study. In blood samples concentrations of serum sL-selectin were determined by an ELISA technique. In colonoscopically obtained biopsies, CD34 expression was evaluated by immunohistochemical methods using monoclonal CD34 antibodies. Disease activity was determined by a clinical semiquantitative scale.RESULTS: sL-selectin levels were found to be significantly increased along with increasing disease activity in UC (p < 0.001) but not in CD (p > 0.05) patients. UC patients with quiescent and severe disease activity had significantly lower (p < 0.005) and higher (p < 0.002) sL-selectin concentrations than controls, respectively. CD34 expression was found to be increased in both disease groups as compared with controls (p < 0.05).CONCLUSION: A disease-specific regulation of CD34 was not found as an explanation for the distinction in sL-selectin regulation. In the light of recent reports on low sL-selectin in other diseases, it is suggested instead that ongoing neutrophil activation may be the reason for low sL-selectin concentrations during quiescent disease stages, whereas chemokine secretion could explain the increased levels of sL-selectin associated with severe disease activity.

AB - OBJECTIVE: Soluble L-selectin (sL-selectin) concentrations are positively correlated with disease activity in ulcerative colitis (UC) but not in Crohn's disease (CD). This difference in sL-selectin regulation could be due to a disease specific regulation of L-selectin ligands. The aim of this study was to compare levels of circulating sL-selectin, expression of the L-selectin ligand CD34 in the affected colon, and inflammatory bowel disease activity.METHODS: Twenty-three patients with UC, 16 patients with CD, and 18 control subjects were included in the study. In blood samples concentrations of serum sL-selectin were determined by an ELISA technique. In colonoscopically obtained biopsies, CD34 expression was evaluated by immunohistochemical methods using monoclonal CD34 antibodies. Disease activity was determined by a clinical semiquantitative scale.RESULTS: sL-selectin levels were found to be significantly increased along with increasing disease activity in UC (p < 0.001) but not in CD (p > 0.05) patients. UC patients with quiescent and severe disease activity had significantly lower (p < 0.005) and higher (p < 0.002) sL-selectin concentrations than controls, respectively. CD34 expression was found to be increased in both disease groups as compared with controls (p < 0.05).CONCLUSION: A disease-specific regulation of CD34 was not found as an explanation for the distinction in sL-selectin regulation. In the light of recent reports on low sL-selectin in other diseases, it is suggested instead that ongoing neutrophil activation may be the reason for low sL-selectin concentrations during quiescent disease stages, whereas chemokine secretion could explain the increased levels of sL-selectin associated with severe disease activity.

KW - Adult

KW - Antigens, CD34

KW - Biopsy

KW - Case-Control Studies

KW - Colitis, Ulcerative

KW - Colon

KW - Crohn Disease

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - L-Selectin

KW - Male

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1572-0241.1998.538_f.x

DO - 10.1111/j.1572-0241.1998.538_f.x

M3 - Journal article

C2 - 9772044

VL - 93

SP - 1854

EP - 1859

JO - The American Journal of Gastroenterology

JF - The American Journal of Gastroenterology

SN - 0002-9270

IS - 10

ER -

ID: 173051741