Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins

Research output: Contribution to journalJournal articlepeer-review

  • Adi Neufeld-Cohen
  • Maria S Robles
  • Rona Aviram
  • Gal Manella
  • Yaarit Adamovich
  • Benjamin Ladeuix
  • Dana Nir
  • Liat Rousso-Noori
  • Yael Kuperman
  • Marina Golik
  • Mann, Matthias
  • Gad Asher

Mitochondria are major suppliers of cellular energy through nutrients oxidation. Little is known about the mechanisms that enable mitochondria to cope with changes in nutrient supply and energy demand that naturally occur throughout the day. To address this question, we applied MS-based quantitative proteomics on isolated mitochondria from mice killed throughout the day and identified extensive oscillations in the mitochondrial proteome. Remarkably, the majority of cycling mitochondrial proteins peaked during the early light phase. We found that rate-limiting mitochondrial enzymes that process lipids and carbohydrates accumulate in a diurnal manner and are dependent on the clock proteins PER1/2. In this conjuncture, we uncovered daily oscillations in mitochondrial respiration that peak during different times of the day in response to different nutrients. Notably, the diurnal regulation of mitochondrial respiration was blunted in mice lacking PER1/2 or on a high-fat diet. We propose that PERIOD proteins optimize mitochondrial metabolism to daily changes in energy supply/demand and thereby, serve as a rheostat for mitochondrial nutrient utilization.

Original languageEnglish
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number12
Pages (from-to)E1673-82
ISSN0027-8424
DOIs
Publication statusPublished - 22 Mar 2016
Externally publishedYes

    Research areas

  • Animals, Circadian Rhythm, Citric Acid Cycle, Diet, High-Fat, Dietary Fats, Electron Transport, Fatty Acids, Feeding Behavior, Gene Expression Profiling, Male, Mice, Mice, Knockout, Mitochondria, Liver, Mitochondrial Proteins, Motor Activity, Period Circadian Proteins, Proteome, RNA, Messenger, Journal Article, Research Support, Non-U.S. Gov't

ID: 186877445