Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle

Research output: Contribution to journal › Journal article › peer-review

Standard

Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle. / Fowler, Veronica; Bashiruddin, John B; Belsham, Graham J; Stenfeldt, Carolina; Bøtner, Anette; Knowles, Nick J; Bankowski, Bartlomiej; Parida, Satya; Barnett, Paul.

In: Veterinary Microbiology, Vol. 169, No. 1-2, 21.02.2014, p. 58-66.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Fowler, V, Bashiruddin, JB, Belsham, GJ, Stenfeldt, C, Bøtner, A, Knowles, NJ, Bankowski, B, Parida, S & Barnett, P 2014, 'Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle', Veterinary Microbiology, vol. 169, no. 1-2, pp. 58-66. https://doi.org/10.1016/j.vetmic.2013.12.008

APA

Fowler, V., Bashiruddin, J. B., Belsham, G. J., Stenfeldt, C., Bøtner, A., Knowles, N. J., Bankowski, B., Parida, S., & Barnett, P. (2014). Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle. Veterinary Microbiology, 169(1-2), 58-66. https://doi.org/10.1016/j.vetmic.2013.12.008

Vancouver

Fowler V, Bashiruddin JB, Belsham GJ, Stenfeldt C, Bøtner A, Knowles NJ et al. Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle. Veterinary Microbiology. 2014 Feb 21;169(1-2):58-66. https://doi.org/10.1016/j.vetmic.2013.12.008

Author

Fowler, Veronica ; Bashiruddin, John B ; Belsham, Graham J ; Stenfeldt, Carolina ; Bøtner, Anette ; Knowles, Nick J ; Bankowski, Bartlomiej ; Parida, Satya ; Barnett, Paul. / Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle. In: Veterinary Microbiology. 2014 ; Vol. 169, No. 1-2. pp. 58-66.

Bibtex

@article{859f99a6b84d4bbb9f0763867025fbd6,

title = "Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle",

abstract = "Previous work in cattle illustrated the protective efficacy and negative marker potential of a A serotype foot-and-mouth disease virus (FMDV) vaccine prepared from a virus lacking a significant portion of the VP1 G-H loop (termed A(-)). Since this deletion also includes the arginine-glycine-aspartate (RGD) motif required for virus attachment to the host cell in vivo, it was hypothesised that this virus would be attentuated in naturally susceptible animals. The A(-) virus was passaged three times in cattle via needle inoculation of virus suspension delivered into the intradermal space of the tongue (intradermolingual: IDL). Included in the study were three direct contact cattle, two of which were used for the third cattle passage (by inoculation) after direct contact exposure for three days. Cattle were monitored for clinical signs and samples were collected for sequencing as well as antibody and viral genome detection by ELISA and qRT-PCR. Following needle inoculation with the A(-) virus, na{\"i}ve cattle developed typical clinical signs of FMDV infection, diagnostic assays also provided positive serological and virological results. However, the contact cattle did not develop clinical signs or generate serological or virological markers indicative of FMDV infection even when the cattle were subsequently needle inoculated with 10(5) TCID50 A(-) FMDV delivered IDL following three days of direct contact exposure. The results suggest that the A(-) virus is not attentuated in cattle when inoculated IDL. This virus could be useful as a tool to understand further the natural pathogenesis, receptor usage and internalisation pathways of FMDV. ",

keywords = "Amino Acid Sequence, Animals, Antibodies, Viral/blood, Capsid Proteins/chemistry, Cattle, Cattle Diseases/immunology, Foot-and-Mouth Disease/immunology, Foot-and-Mouth Disease Virus/genetics, Male, Sequence Deletion, Tongue/virology, Vaccination",

author = "Veronica Fowler and Bashiruddin, {John B} and Belsham, {Graham J} and Carolina Stenfeldt and Anette B{\o}tner and Knowles, {Nick J} and Bartlomiej Bankowski and Satya Parida and Paul Barnett",

year = "2014",

month = feb,

day = "21",

doi = "10.1016/j.vetmic.2013.12.008",

language = "English",

volume = "169",

pages = "58--66",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier",

number = "1-2",

}

RIS

TY - JOUR

T1 - Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle

AU - Fowler, Veronica

AU - Bashiruddin, John B

AU - Belsham, Graham J

AU - Stenfeldt, Carolina

AU - Bøtner, Anette

AU - Knowles, Nick J

AU - Bankowski, Bartlomiej

AU - Parida, Satya

AU - Barnett, Paul

PY - 2014/2/21

Y1 - 2014/2/21

N2 - Previous work in cattle illustrated the protective efficacy and negative marker potential of a A serotype foot-and-mouth disease virus (FMDV) vaccine prepared from a virus lacking a significant portion of the VP1 G-H loop (termed A(-)). Since this deletion also includes the arginine-glycine-aspartate (RGD) motif required for virus attachment to the host cell in vivo, it was hypothesised that this virus would be attentuated in naturally susceptible animals. The A(-) virus was passaged three times in cattle via needle inoculation of virus suspension delivered into the intradermal space of the tongue (intradermolingual: IDL). Included in the study were three direct contact cattle, two of which were used for the third cattle passage (by inoculation) after direct contact exposure for three days. Cattle were monitored for clinical signs and samples were collected for sequencing as well as antibody and viral genome detection by ELISA and qRT-PCR. Following needle inoculation with the A(-) virus, naïve cattle developed typical clinical signs of FMDV infection, diagnostic assays also provided positive serological and virological results. However, the contact cattle did not develop clinical signs or generate serological or virological markers indicative of FMDV infection even when the cattle were subsequently needle inoculated with 10(5) TCID50 A(-) FMDV delivered IDL following three days of direct contact exposure. The results suggest that the A(-) virus is not attentuated in cattle when inoculated IDL. This virus could be useful as a tool to understand further the natural pathogenesis, receptor usage and internalisation pathways of FMDV.

AB - Previous work in cattle illustrated the protective efficacy and negative marker potential of a A serotype foot-and-mouth disease virus (FMDV) vaccine prepared from a virus lacking a significant portion of the VP1 G-H loop (termed A(-)). Since this deletion also includes the arginine-glycine-aspartate (RGD) motif required for virus attachment to the host cell in vivo, it was hypothesised that this virus would be attentuated in naturally susceptible animals. The A(-) virus was passaged three times in cattle via needle inoculation of virus suspension delivered into the intradermal space of the tongue (intradermolingual: IDL). Included in the study were three direct contact cattle, two of which were used for the third cattle passage (by inoculation) after direct contact exposure for three days. Cattle were monitored for clinical signs and samples were collected for sequencing as well as antibody and viral genome detection by ELISA and qRT-PCR. Following needle inoculation with the A(-) virus, naïve cattle developed typical clinical signs of FMDV infection, diagnostic assays also provided positive serological and virological results. However, the contact cattle did not develop clinical signs or generate serological or virological markers indicative of FMDV infection even when the cattle were subsequently needle inoculated with 10(5) TCID50 A(-) FMDV delivered IDL following three days of direct contact exposure. The results suggest that the A(-) virus is not attentuated in cattle when inoculated IDL. This virus could be useful as a tool to understand further the natural pathogenesis, receptor usage and internalisation pathways of FMDV.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Viral/blood

KW - Capsid Proteins/chemistry

KW - Cattle

KW - Cattle Diseases/immunology

KW - Foot-and-Mouth Disease/immunology

KW - Foot-and-Mouth Disease Virus/genetics

KW - Male

KW - Sequence Deletion

KW - Tongue/virology

KW - Vaccination

U2 - 10.1016/j.vetmic.2013.12.008

DO - 10.1016/j.vetmic.2013.12.008

M3 - Journal article

C2 - 24438986

VL - 169

SP - 58

EP - 66

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

ER -

ID: 257916142