Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs. / Mirza, Aashiq H.; Kaur, Simranjeet; Nielsen, Lotte B.; Størling, Joachim; Yarani, Reza; Roursgaard, Martin; Mathiesen, Elisabeth R.; Damm, Peter; Svare, Jens; Mortensen, Henrik B.; Pociot, Flemming.

In: Frontiers in Immunology, Vol. 10, 2019, p. 2543.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Mirza, AH, Kaur, S, Nielsen, LB, Størling, J, Yarani, R, Roursgaard, M, Mathiesen, ER, Damm, P, Svare, J, Mortensen, HB & Pociot, F 2019, 'Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs', Frontiers in Immunology, vol. 10, pp. 2543. https://doi.org/10.3389/fimmu.2019.02543

APA

Mirza, A. H., Kaur, S., Nielsen, L. B., Størling, J., Yarani, R., Roursgaard, M., ... Pociot, F. (2019). Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs. Frontiers in Immunology, 10, 2543. https://doi.org/10.3389/fimmu.2019.02543

Vancouver

Mirza AH, Kaur S, Nielsen LB, Størling J, Yarani R, Roursgaard M et al. Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs. Frontiers in Immunology. 2019;10:2543. https://doi.org/10.3389/fimmu.2019.02543

Author

Mirza, Aashiq H. ; Kaur, Simranjeet ; Nielsen, Lotte B. ; Størling, Joachim ; Yarani, Reza ; Roursgaard, Martin ; Mathiesen, Elisabeth R. ; Damm, Peter ; Svare, Jens ; Mortensen, Henrik B. ; Pociot, Flemming. / Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs. In: Frontiers in Immunology. 2019 ; Vol. 10. pp. 2543.

Bibtex

@article{67cf935c68c343be81127b4b41e9d138,
title = "Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs",
abstract = "The breast milk plays a crucial role in shaping the initial intestinal microbiota and mucosal immunity of the infant. Interestingly, breastfeeding has proven to be protective against the early onset of immune-mediated diseases including type 1 diabetes. Studies have shown that exosomes from human breast milk are enriched in immune-modulating miRNAs suggesting that exosomal miRNAs (exomiRs) transferred to the infant could play a critical role in the development of the infant's immune system. We extracted exomiRs from breast milk of 52 lactating mothers (26 mothers with type 1 diabetes and 26 healthy mothers), to identify any differences in the exomiR content between the two groups. Small RNA-sequencing was performed to identify known and novel miRNAs in both groups. A total of 631 exomiRs were detected by small RNA sequencing including immune-related miRNAs such as hsa-let-7c, hsa-miR-21, hsa-miR-34a, hsa-miR-146b, and hsa-miR-200b. In addition, ~200 novel miRNAs were identified in both type 1 diabetes and control samples. Among the known miRNAs, nine exomiR's were found differentially expressed in mothers with type 1 diabetes compared to healthy mothers. The highly up-regulated miRNAs, hsa-miR-4497, and hsa-miR-3178, increased lipopolysaccharide-induced expression and secretion of tumor necrosis factor α (TNFα) in human monocytes. The up-regulated miRNA target genes were significantly enriched for longevity-regulating pathways and FoxO signaling. Our findings suggest a role of breast milk-derived exomiRs in modulating the infant's immune system.",
keywords = "breast milk, exosomes, miRNAs, Type 1 diabetes, exomiRs",
author = "Mirza, {Aashiq H.} and Simranjeet Kaur and Nielsen, {Lotte B.} and Joachim St{\o}rling and Reza Yarani and Martin Roursgaard and Mathiesen, {Elisabeth R.} and Peter Damm and Jens Svare and Mortensen, {Henrik B.} and Flemming Pociot",
year = "2019",
doi = "10.3389/fimmu.2019.02543",
language = "English",
volume = "10",
pages = "2543",
journal = "Frontiers in Immunology",
issn = "1664-3224",
publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs

AU - Mirza, Aashiq H.

AU - Kaur, Simranjeet

AU - Nielsen, Lotte B.

AU - Størling, Joachim

AU - Yarani, Reza

AU - Roursgaard, Martin

AU - Mathiesen, Elisabeth R.

AU - Damm, Peter

AU - Svare, Jens

AU - Mortensen, Henrik B.

AU - Pociot, Flemming

PY - 2019

Y1 - 2019

N2 - The breast milk plays a crucial role in shaping the initial intestinal microbiota and mucosal immunity of the infant. Interestingly, breastfeeding has proven to be protective against the early onset of immune-mediated diseases including type 1 diabetes. Studies have shown that exosomes from human breast milk are enriched in immune-modulating miRNAs suggesting that exosomal miRNAs (exomiRs) transferred to the infant could play a critical role in the development of the infant's immune system. We extracted exomiRs from breast milk of 52 lactating mothers (26 mothers with type 1 diabetes and 26 healthy mothers), to identify any differences in the exomiR content between the two groups. Small RNA-sequencing was performed to identify known and novel miRNAs in both groups. A total of 631 exomiRs were detected by small RNA sequencing including immune-related miRNAs such as hsa-let-7c, hsa-miR-21, hsa-miR-34a, hsa-miR-146b, and hsa-miR-200b. In addition, ~200 novel miRNAs were identified in both type 1 diabetes and control samples. Among the known miRNAs, nine exomiR's were found differentially expressed in mothers with type 1 diabetes compared to healthy mothers. The highly up-regulated miRNAs, hsa-miR-4497, and hsa-miR-3178, increased lipopolysaccharide-induced expression and secretion of tumor necrosis factor α (TNFα) in human monocytes. The up-regulated miRNA target genes were significantly enriched for longevity-regulating pathways and FoxO signaling. Our findings suggest a role of breast milk-derived exomiRs in modulating the infant's immune system.

AB - The breast milk plays a crucial role in shaping the initial intestinal microbiota and mucosal immunity of the infant. Interestingly, breastfeeding has proven to be protective against the early onset of immune-mediated diseases including type 1 diabetes. Studies have shown that exosomes from human breast milk are enriched in immune-modulating miRNAs suggesting that exosomal miRNAs (exomiRs) transferred to the infant could play a critical role in the development of the infant's immune system. We extracted exomiRs from breast milk of 52 lactating mothers (26 mothers with type 1 diabetes and 26 healthy mothers), to identify any differences in the exomiR content between the two groups. Small RNA-sequencing was performed to identify known and novel miRNAs in both groups. A total of 631 exomiRs were detected by small RNA sequencing including immune-related miRNAs such as hsa-let-7c, hsa-miR-21, hsa-miR-34a, hsa-miR-146b, and hsa-miR-200b. In addition, ~200 novel miRNAs were identified in both type 1 diabetes and control samples. Among the known miRNAs, nine exomiR's were found differentially expressed in mothers with type 1 diabetes compared to healthy mothers. The highly up-regulated miRNAs, hsa-miR-4497, and hsa-miR-3178, increased lipopolysaccharide-induced expression and secretion of tumor necrosis factor α (TNFα) in human monocytes. The up-regulated miRNA target genes were significantly enriched for longevity-regulating pathways and FoxO signaling. Our findings suggest a role of breast milk-derived exomiRs in modulating the infant's immune system.

KW - breast milk

KW - exosomes

KW - miRNAs

KW - Type 1 diabetes

KW - exomiRs

U2 - 10.3389/fimmu.2019.02543

DO - 10.3389/fimmu.2019.02543

M3 - Journal article

C2 - 31708933

VL - 10

SP - 2543

JO - Frontiers in Immunology

JF - Frontiers in Immunology

SN - 1664-3224

ER -

ID: 230473998