Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics
Research output: Contribution to journal › Journal article › Research › peer-review
Single nucleotide polymorphism genotyping provides a supplement for conventional short tandem repeats-based kits currently used for human identification. GenPlex (Applied Biosystems (AB), Foster City, CA) is an SNP-genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement, and validate a small and affordable automated liquid-handling robot for forensic casework samples (buccal swaps on FTA-paper and Qiagen purified blood). The reaction scheme consisted of numerous steps and was cumbersome to perform consistently manually. Automation was accomplished with a Biomek-3000 (Beckmann Coulter) laboratory-automated workstation using five in-house-developed methods. All methods allowed the user to select the number of subsequent injections to the capillary electrophoresis instrument (ABI 3130xl, AB) enabling processing of both partial and full plates. A total of 286 samples were analyzed in duplicates with the GenPlex reaction using the Biomek-3000. The results were compared with those obtained from the same samples using the SNaPshot(AB) single-base extension system. Full concordance of the results was obtained in all but one sample. The results demonstrate that the Biomek-3000 can perform a series of complex reactions leading to highly consistent forensic genetic SNP-typing results
Udgivelsesdato: 2008/10
Udgivelsesdato: 2008/10
Original language | English |
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Journal | JALA - Journal of the Association for Laboratory Automation |
Volume | 13 |
Issue number | 5 |
Pages (from-to) | 297-303 |
ISSN | 1535-5535 |
DOIs | |
Publication status | Published - 2008 |
ID: 14440048