Bile acids drive colonic secretion of glucagon-like-peptide 1 and peptide-YY in rodents
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Bile acids drive colonic secretion of glucagon-like-peptide 1 and peptide-YY in rodents. / Christiansen, Charlotte Bayer; Trammell, Samuel Addison Jack; Albrechtsen, Nicolai Jacob Wewer; Schoonjans, Kristina; Albrechtsen, Reidar; Gillum, Matthew Paul; Kuhre, Rune Ehrenreich; Holst, Jens Juul.
In: American Journal of Physiology: Gastrointestinal and Liver Physiology, Vol. 316, No. 5, 2019, p. G574-G584.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Bile acids drive colonic secretion of glucagon-like-peptide 1 and peptide-YY in rodents
AU - Christiansen, Charlotte Bayer
AU - Trammell, Samuel Addison Jack
AU - Albrechtsen, Nicolai Jacob Wewer
AU - Schoonjans, Kristina
AU - Albrechtsen, Reidar
AU - Gillum, Matthew Paul
AU - Kuhre, Rune Ehrenreich
AU - Holst, Jens Juul
PY - 2019
Y1 - 2019
N2 - A large number of glucagon-like-peptide 1 (GLP-1) and peptide-YY (PYY) producing L-cells are located in the colon, but little is known about their contribution to whole body metabolism. Since bile acids (BAs) increase GLP-1 and PYY release, and since BAs spill over from the ileum to the colon, we decided to investigate the ability of BAs to stimulate colonic GLP-1 and PYY secretion. Using isolated perfused rat/mouse colon as well as stimulation of the rat colon in vivo, we demonstrate that BAs significantly enhance secretion of GLP-1 and PYY from the colon with average increases of 3.5 and 2.9-fold, respectively. Furthermore, we find that responses depend on BA absorption followed by basolateral activation of the BA-receptor, TGR5. Surprisingly, the apical sodium-dependent-bile-acid-transporter (IBAT), which serves to absorb conjugated BAs, was not required for colonic conjugated BA absorption or conjugated BA-induced peptide secretion. In conclusion, we demonstrate that BAs represent a major physiological stimulus for colonic L-cell secretion.
AB - A large number of glucagon-like-peptide 1 (GLP-1) and peptide-YY (PYY) producing L-cells are located in the colon, but little is known about their contribution to whole body metabolism. Since bile acids (BAs) increase GLP-1 and PYY release, and since BAs spill over from the ileum to the colon, we decided to investigate the ability of BAs to stimulate colonic GLP-1 and PYY secretion. Using isolated perfused rat/mouse colon as well as stimulation of the rat colon in vivo, we demonstrate that BAs significantly enhance secretion of GLP-1 and PYY from the colon with average increases of 3.5 and 2.9-fold, respectively. Furthermore, we find that responses depend on BA absorption followed by basolateral activation of the BA-receptor, TGR5. Surprisingly, the apical sodium-dependent-bile-acid-transporter (IBAT), which serves to absorb conjugated BAs, was not required for colonic conjugated BA absorption or conjugated BA-induced peptide secretion. In conclusion, we demonstrate that BAs represent a major physiological stimulus for colonic L-cell secretion.
U2 - 10.1152/ajpgi.00010.2019
DO - 10.1152/ajpgi.00010.2019
M3 - Journal article
C2 - 30767682
VL - 316
SP - G574-G584
JO - American Journal of Physiology: Gastrointestinal and Liver Physiology
JF - American Journal of Physiology: Gastrointestinal and Liver Physiology
SN - 0193-1857
IS - 5
ER -
ID: 214748361