Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes
Research output: Contribution to journal › Journal article › Research › peer-review
We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.
Original language | English |
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Journal | Infection and Immunity |
Volume | 74 |
Issue number | 7 |
Pages (from-to) | 4357-60 |
Number of pages | 3 |
ISSN | 0019-9567 |
DOIs | |
Publication status | Published - 2006 |
Bibliographical note
Keywords: Animals; Antigens, Protozoan; Baculoviridae; Erythrocytes; Female; Genetic Vectors; Humans; Immune Sera; Immunoglobulin G; Mice; Plasmodium falciparum; Rabbits; Recombinant Proteins
ID: 6746578